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Report on the first detection of pectenotoxin-2, spirolide-a and their derivatives in French shellfish.

Amzil Z, Sibat M, Royer F, Masson N, Abadie E - Mar Drugs (2007)

Bottom Line: The results revealed different lipophilic toxin profiles as a function of the shellfish sampling location.In addition, other lipophilic toxins were found in shellfish samples: DTX-2, acyl-DTX-2 and SPXs (SPX-A, SPX-desMeC) on the Atlantic coast (Southern Brittany, Arcachon), and pectenotoxins (PTX-2, PTX-2-seco-acid and 7-epi-PTX-2-seco-acid) on the Mediterranean coast (Thau lagoon, the island of Corsica).This paper reports on the first detection of PTX-2, SPX-A and their derivatives in French shellfish.

View Article: PubMed Central - PubMed

Affiliation: IFREMER, Microbiology and Phycotoxins Department, BP 21105, 44311 Nantes, France. Zouher.Amzil@ifremer.fr

ABSTRACT
In the context of the French Phytoplankton and Phycotoxins Monitoring Network (REPHY) programme, shellfish samples were harvested from different locations where harmful algae blooms were known to have occurred. For all shellfish samples found positive by the mouse bioassay for diarrhetic shellfish poisoning (DSP) toxins, liquid chromatography (LC) coupled with mass spectrometry (MS) was used to search for the following lipophilic toxins: okadaic acid (OA), dinophysistoxins (DTXs), pectenotoxins (PTXs), azaspiracids (AZAs), yessotoxins (YTXs), spirolides (SPXs) and gymnodimines (GYMs). In order to investigate the presence of acyl-OAs and/or acyl-DTX-1,-2 (DTX-3), alkaline hydrolysis was performed on all samples, and LC/MS analyses were carried out on the samples before and after hydrolysis. The results revealed different lipophilic toxin profiles as a function of the shellfish sampling location. The primary finding was that all of the samples contained OA and acyl-OA. In addition, other lipophilic toxins were found in shellfish samples: DTX-2, acyl-DTX-2 and SPXs (SPX-A, SPX-desMeC) on the Atlantic coast (Southern Brittany, Arcachon), and pectenotoxins (PTX-2, PTX-2-seco-acid and 7-epi-PTX-2-seco-acid) on the Mediterranean coast (Thau lagoon, the island of Corsica). This paper reports on the first detection of PTX-2, SPX-A and their derivatives in French shellfish.

No MeSH data available.


Related in: MedlinePlus

Extraction procedure for lipophilic toxin analysis by LC-MS/MS
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Related In: Results  -  Collection


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f1-md504168: Extraction procedure for lipophilic toxin analysis by LC-MS/MS

Mentions: Figure 1 shows the complete extraction procedure: lipophilic toxins were extracted from HP homogenate (2 g) according to the DSP mouse-assay protocol, already optimized with MUS-2 reference material. The resulting CH2Cl2 phase was then dried and diluted in MeOH. One aliquot was used for lipophilic toxins (except acyls-esters “DTX-3”) analysis, and a second aliquot for DTX-3 alkaline hydrolysis to release OA, DTX-1, -2, and subsequent analysis of DTX3.


Report on the first detection of pectenotoxin-2, spirolide-a and their derivatives in French shellfish.

Amzil Z, Sibat M, Royer F, Masson N, Abadie E - Mar Drugs (2007)

Extraction procedure for lipophilic toxin analysis by LC-MS/MS
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2365699&req=5

f1-md504168: Extraction procedure for lipophilic toxin analysis by LC-MS/MS
Mentions: Figure 1 shows the complete extraction procedure: lipophilic toxins were extracted from HP homogenate (2 g) according to the DSP mouse-assay protocol, already optimized with MUS-2 reference material. The resulting CH2Cl2 phase was then dried and diluted in MeOH. One aliquot was used for lipophilic toxins (except acyls-esters “DTX-3”) analysis, and a second aliquot for DTX-3 alkaline hydrolysis to release OA, DTX-1, -2, and subsequent analysis of DTX3.

Bottom Line: The results revealed different lipophilic toxin profiles as a function of the shellfish sampling location.In addition, other lipophilic toxins were found in shellfish samples: DTX-2, acyl-DTX-2 and SPXs (SPX-A, SPX-desMeC) on the Atlantic coast (Southern Brittany, Arcachon), and pectenotoxins (PTX-2, PTX-2-seco-acid and 7-epi-PTX-2-seco-acid) on the Mediterranean coast (Thau lagoon, the island of Corsica).This paper reports on the first detection of PTX-2, SPX-A and their derivatives in French shellfish.

View Article: PubMed Central - PubMed

Affiliation: IFREMER, Microbiology and Phycotoxins Department, BP 21105, 44311 Nantes, France. Zouher.Amzil@ifremer.fr

ABSTRACT
In the context of the French Phytoplankton and Phycotoxins Monitoring Network (REPHY) programme, shellfish samples were harvested from different locations where harmful algae blooms were known to have occurred. For all shellfish samples found positive by the mouse bioassay for diarrhetic shellfish poisoning (DSP) toxins, liquid chromatography (LC) coupled with mass spectrometry (MS) was used to search for the following lipophilic toxins: okadaic acid (OA), dinophysistoxins (DTXs), pectenotoxins (PTXs), azaspiracids (AZAs), yessotoxins (YTXs), spirolides (SPXs) and gymnodimines (GYMs). In order to investigate the presence of acyl-OAs and/or acyl-DTX-1,-2 (DTX-3), alkaline hydrolysis was performed on all samples, and LC/MS analyses were carried out on the samples before and after hydrolysis. The results revealed different lipophilic toxin profiles as a function of the shellfish sampling location. The primary finding was that all of the samples contained OA and acyl-OA. In addition, other lipophilic toxins were found in shellfish samples: DTX-2, acyl-DTX-2 and SPXs (SPX-A, SPX-desMeC) on the Atlantic coast (Southern Brittany, Arcachon), and pectenotoxins (PTX-2, PTX-2-seco-acid and 7-epi-PTX-2-seco-acid) on the Mediterranean coast (Thau lagoon, the island of Corsica). This paper reports on the first detection of PTX-2, SPX-A and their derivatives in French shellfish.

No MeSH data available.


Related in: MedlinePlus