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Snail accelerates cancer invasion by upregulating MMP expression and is associated with poor prognosis of hepatocellular carcinoma.

Miyoshi A, Kitajima Y, Kido S, Shimonishi T, Matsuyama S, Kitahara K, Miyazaki K - Br. J. Cancer (2005)

Bottom Line: In the ordinary HCC tissues, Snail expression was significantly correlated with portal vein invasion (P=0.035) and intrahepatic metastasis (P=0.050); it also showed a significant correlation with MT1-MMP expression (r=0.572, P<0.001).In recurrence-free survival, the group with high Snail expression showed significantly poorer prognosis (P=0.035).During the progression of HCC, Snail expression may be induced and accelerate invasion activity by upregulating MMP expression, resulting in portal invasion, intrahepatic metastasis, and poor prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Saga University Faculty of Medicine, 5-1-1 Nabeshima, Saga 849-8501, Japan.

ABSTRACT
We have previously demonstrated in an in vitro study that Snail increased the invasion activity of hepatoma cells by upregulating matrix metalloproteinase (MMP) gene expression. In the present study, we examined whether Snail gene expression correlates with cancer invasion and prognosis of patients with hepatocellular carcinoma (HCC). Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed to evaluate Snail, E-cadherin, and MMP mRNA expressions in eight nodule-in-nodule tumours and 47 ordinary HCC tissues. In the nodule-in-nodule tumours, Snail expression significantly increased with tumour dedifferentiation (P=0.047). In the ordinary HCC tissues, Snail expression was significantly correlated with portal vein invasion (P=0.035) and intrahepatic metastasis (P=0.050); it also showed a significant correlation with MT1-MMP expression (r=0.572, P<0.001). In recurrence-free survival, the group with high Snail expression showed significantly poorer prognosis (P=0.035). Moreover, high Snail expression was an independent risk factor for early recurrence after curative resection. During the progression of HCC, Snail expression may be induced and accelerate invasion activity by upregulating MMP expression, resulting in portal invasion, intrahepatic metastasis, and poor prognosis.

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Macroscopic and microscopic features of HCC showing a NIN appearance. (A) Macroscopically, the solitary tumour was separated into an outer nodule and an inner nodule by a septum. (B) Microscopically, the outer nodule showed a moderately differentiated HCC, forming in a pseudoglandular fashion. (C) Hepatocellular carcinoma cells in the inner nodule were poorly differentiated with large hyperchromatic nuclei. Microscopic photographs were taken at a magnification of × 200.
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fig1: Macroscopic and microscopic features of HCC showing a NIN appearance. (A) Macroscopically, the solitary tumour was separated into an outer nodule and an inner nodule by a septum. (B) Microscopically, the outer nodule showed a moderately differentiated HCC, forming in a pseudoglandular fashion. (C) Hepatocellular carcinoma cells in the inner nodule were poorly differentiated with large hyperchromatic nuclei. Microscopic photographs were taken at a magnification of × 200.

Mentions: Among the 55 patients remaining in the study, eight patients had tumours with a nodule-in-nodule appearance (NIN) on macroscopic and microscopic findings (Figure 1). Hepatocellular carcinoma with NIN was better tissue for investigating the dedifferentiation and progression of HCC because the inner nodule of less differentiation developed sequentially from the well-differentiated outer nodule with the same genetic background (Kojiro, 1998; Midorikawa et al, 2002).


Snail accelerates cancer invasion by upregulating MMP expression and is associated with poor prognosis of hepatocellular carcinoma.

Miyoshi A, Kitajima Y, Kido S, Shimonishi T, Matsuyama S, Kitahara K, Miyazaki K - Br. J. Cancer (2005)

Macroscopic and microscopic features of HCC showing a NIN appearance. (A) Macroscopically, the solitary tumour was separated into an outer nodule and an inner nodule by a septum. (B) Microscopically, the outer nodule showed a moderately differentiated HCC, forming in a pseudoglandular fashion. (C) Hepatocellular carcinoma cells in the inner nodule were poorly differentiated with large hyperchromatic nuclei. Microscopic photographs were taken at a magnification of × 200.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2361838&req=5

fig1: Macroscopic and microscopic features of HCC showing a NIN appearance. (A) Macroscopically, the solitary tumour was separated into an outer nodule and an inner nodule by a septum. (B) Microscopically, the outer nodule showed a moderately differentiated HCC, forming in a pseudoglandular fashion. (C) Hepatocellular carcinoma cells in the inner nodule were poorly differentiated with large hyperchromatic nuclei. Microscopic photographs were taken at a magnification of × 200.
Mentions: Among the 55 patients remaining in the study, eight patients had tumours with a nodule-in-nodule appearance (NIN) on macroscopic and microscopic findings (Figure 1). Hepatocellular carcinoma with NIN was better tissue for investigating the dedifferentiation and progression of HCC because the inner nodule of less differentiation developed sequentially from the well-differentiated outer nodule with the same genetic background (Kojiro, 1998; Midorikawa et al, 2002).

Bottom Line: In the ordinary HCC tissues, Snail expression was significantly correlated with portal vein invasion (P=0.035) and intrahepatic metastasis (P=0.050); it also showed a significant correlation with MT1-MMP expression (r=0.572, P<0.001).In recurrence-free survival, the group with high Snail expression showed significantly poorer prognosis (P=0.035).During the progression of HCC, Snail expression may be induced and accelerate invasion activity by upregulating MMP expression, resulting in portal invasion, intrahepatic metastasis, and poor prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Saga University Faculty of Medicine, 5-1-1 Nabeshima, Saga 849-8501, Japan.

ABSTRACT
We have previously demonstrated in an in vitro study that Snail increased the invasion activity of hepatoma cells by upregulating matrix metalloproteinase (MMP) gene expression. In the present study, we examined whether Snail gene expression correlates with cancer invasion and prognosis of patients with hepatocellular carcinoma (HCC). Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed to evaluate Snail, E-cadherin, and MMP mRNA expressions in eight nodule-in-nodule tumours and 47 ordinary HCC tissues. In the nodule-in-nodule tumours, Snail expression significantly increased with tumour dedifferentiation (P=0.047). In the ordinary HCC tissues, Snail expression was significantly correlated with portal vein invasion (P=0.035) and intrahepatic metastasis (P=0.050); it also showed a significant correlation with MT1-MMP expression (r=0.572, P<0.001). In recurrence-free survival, the group with high Snail expression showed significantly poorer prognosis (P=0.035). Moreover, high Snail expression was an independent risk factor for early recurrence after curative resection. During the progression of HCC, Snail expression may be induced and accelerate invasion activity by upregulating MMP expression, resulting in portal invasion, intrahepatic metastasis, and poor prognosis.

Show MeSH
Related in: MedlinePlus