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Decreased expression of haemoglobin beta (HBB) gene in anaplastic thyroid cancer and recovery of its expression inhibits cell growth.

Onda M, Akaishi J, Asaka S, Okamoto J, Miyamoto S, Mizutani K, Yoshida A, Ito K, Emi M - Br. J. Cancer (2005)

Bottom Line: Haemoglobin beta showed significant decreased expression in ACLs and ATCs; however, in PTC, HBB expressed equal to the normal thyroid gland.Forced expression of HBB in KTA2 cell, which is a kind of ACL, significantly suppressed KTA2 growth.The mechanism of downregulation of HBB in ATC is still unclear; however, our results suggested the possibility of HBB as a novel tumour-suppressor gene.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Institute of Gerontology, Nippon Medical School, Kawasaki, Japan. monda@nms.ac.jp

ABSTRACT
Anaplastic thyroid cancer (ATC) is one of the most fulminant and foetal diseases in human malignancies. However, the genetic alterations and carcinogenic mechanisms of ATC are still unclear. Recently, we investigated the gene expression profile of 11 anaplastic thyroid cancer cell lines (ACL) and significant decreased expression of haemoglobin beta (HBB) gene in ACL. Haemoglobin beta is located at 11p15.5, where loss of heterozygosity (LOH) was reported in various kinds of cancers, including ATC, and it has been suggested that novel tumour suppressor genes might exist in this region. In order to clarify the meaning of decreased expression of HBB in ATC, the expression status of HBB was investigated with ACL, ATC, papillary thyroid cancer (PTC) and normal human tissues. Haemoglobin beta showed significant decreased expression in ACLs and ATCs; however, in PTC, HBB expressed equal to the normal thyroid gland. In addition, HBB expressed in normal human tissues ubiquitously. To validate the tumour-suppressor function of HBB, cell growth assay was performed. Forced expression of HBB in KTA2 cell, which is a kind of ACL, significantly suppressed KTA2 growth. The mechanism of downregulation of HBB in ATC is still unclear; however, our results suggested the possibility of HBB as a novel tumour-suppressor gene.

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(A) Expression of HBB in ACLs. Compared to five normal thyroid gland expressions, HBB expression was significantly decreased in ACL samples. M: size marker, N1–5: normal thyroid, 1: 8305c, 2: 8505c, 3: ARO, 4: FRO, 5: TTA1, 6: TTA2, 7: TTA3, 8: KTA1, 9: KTA2, 10: KTA3, 11: KTA4. (B) Result of Q-PCR. The average expression of five normal thyroid glands was settled as 1.00, and relative expression ratio compared to normal was calculated. Average expression of HBB in ACL was below 1% of normal thyroid gland.
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fig1: (A) Expression of HBB in ACLs. Compared to five normal thyroid gland expressions, HBB expression was significantly decreased in ACL samples. M: size marker, N1–5: normal thyroid, 1: 8305c, 2: 8505c, 3: ARO, 4: FRO, 5: TTA1, 6: TTA2, 7: TTA3, 8: KTA1, 9: KTA2, 10: KTA3, 11: KTA4. (B) Result of Q-PCR. The average expression of five normal thyroid glands was settled as 1.00, and relative expression ratio compared to normal was calculated. Average expression of HBB in ACL was below 1% of normal thyroid gland.

Mentions: In order to evaluate the expression of HBB in ACLs, SQ-PCR and Q-PCR were performed. Compared to normal thyroid glands that were derived from five PTC patients, expression of HBB was significantly decreased in 11 ACLs (Figure 1A and B). Average expression of HBB in ACL was below 1% of the average expression in a normal thyroid gland.


Decreased expression of haemoglobin beta (HBB) gene in anaplastic thyroid cancer and recovery of its expression inhibits cell growth.

Onda M, Akaishi J, Asaka S, Okamoto J, Miyamoto S, Mizutani K, Yoshida A, Ito K, Emi M - Br. J. Cancer (2005)

(A) Expression of HBB in ACLs. Compared to five normal thyroid gland expressions, HBB expression was significantly decreased in ACL samples. M: size marker, N1–5: normal thyroid, 1: 8305c, 2: 8505c, 3: ARO, 4: FRO, 5: TTA1, 6: TTA2, 7: TTA3, 8: KTA1, 9: KTA2, 10: KTA3, 11: KTA4. (B) Result of Q-PCR. The average expression of five normal thyroid glands was settled as 1.00, and relative expression ratio compared to normal was calculated. Average expression of HBB in ACL was below 1% of normal thyroid gland.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2361827&req=5

fig1: (A) Expression of HBB in ACLs. Compared to five normal thyroid gland expressions, HBB expression was significantly decreased in ACL samples. M: size marker, N1–5: normal thyroid, 1: 8305c, 2: 8505c, 3: ARO, 4: FRO, 5: TTA1, 6: TTA2, 7: TTA3, 8: KTA1, 9: KTA2, 10: KTA3, 11: KTA4. (B) Result of Q-PCR. The average expression of five normal thyroid glands was settled as 1.00, and relative expression ratio compared to normal was calculated. Average expression of HBB in ACL was below 1% of normal thyroid gland.
Mentions: In order to evaluate the expression of HBB in ACLs, SQ-PCR and Q-PCR were performed. Compared to normal thyroid glands that were derived from five PTC patients, expression of HBB was significantly decreased in 11 ACLs (Figure 1A and B). Average expression of HBB in ACL was below 1% of the average expression in a normal thyroid gland.

Bottom Line: Haemoglobin beta showed significant decreased expression in ACLs and ATCs; however, in PTC, HBB expressed equal to the normal thyroid gland.Forced expression of HBB in KTA2 cell, which is a kind of ACL, significantly suppressed KTA2 growth.The mechanism of downregulation of HBB in ATC is still unclear; however, our results suggested the possibility of HBB as a novel tumour-suppressor gene.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Institute of Gerontology, Nippon Medical School, Kawasaki, Japan. monda@nms.ac.jp

ABSTRACT
Anaplastic thyroid cancer (ATC) is one of the most fulminant and foetal diseases in human malignancies. However, the genetic alterations and carcinogenic mechanisms of ATC are still unclear. Recently, we investigated the gene expression profile of 11 anaplastic thyroid cancer cell lines (ACL) and significant decreased expression of haemoglobin beta (HBB) gene in ACL. Haemoglobin beta is located at 11p15.5, where loss of heterozygosity (LOH) was reported in various kinds of cancers, including ATC, and it has been suggested that novel tumour suppressor genes might exist in this region. In order to clarify the meaning of decreased expression of HBB in ATC, the expression status of HBB was investigated with ACL, ATC, papillary thyroid cancer (PTC) and normal human tissues. Haemoglobin beta showed significant decreased expression in ACLs and ATCs; however, in PTC, HBB expressed equal to the normal thyroid gland. In addition, HBB expressed in normal human tissues ubiquitously. To validate the tumour-suppressor function of HBB, cell growth assay was performed. Forced expression of HBB in KTA2 cell, which is a kind of ACL, significantly suppressed KTA2 growth. The mechanism of downregulation of HBB in ATC is still unclear; however, our results suggested the possibility of HBB as a novel tumour-suppressor gene.

Show MeSH
Related in: MedlinePlus