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Type distribution, viral load and integration status of high-risk human papillomaviruses in pre-stages of cervical cancer (CIN).

Andersson S, Safari H, Mints M, Lewensohn-Fuchs I, Gyllensten U, Johansson B - Br. J. Cancer (2005)

Bottom Line: Two methods for the extraction of DNA from the paraffin-embedded biopsies were compared: a protocol based on the MagNA pure system (Roche) and a Qiagen spin column kit (Qiagen).HPV 16 was the most prevalent, and was found in 20, 28 and 46% of the CIN I, II and III cases, respectively.All samples were found to contain integrated forms of HPV 16, frequently mixed with an episomal form.

View Article: PubMed Central - PubMed

Affiliation: Institute for Clinical Science, Division of Obstetrics and Gynaecology, Karolinska University Hospital, Huddinge, Karolinska Institute, 141 86 Stockholm, Sweden. sonia.andersson@telia.com

ABSTRACT
A series of 176 archival cervical intraepithelial neoplasia (CIN) was analysed for the presence, viral load and integration status of 'high-risk' types of human papillomavirus (HR-HPV). The samples were assayed using newly developed methods based on real-time PCR. Two methods for the extraction of DNA from the paraffin-embedded biopsies were compared: a protocol based on the MagNA pure system (Roche) and a Qiagen spin column kit (Qiagen). It was possible to amplify 94% (166) of the samples. Of these, 36, 63 and 80% of the CIN I, II and III cases contained HR-HPV. HPV 16 was the most prevalent, and was found in 20, 28 and 46% of the CIN I, II and III cases, respectively. The second most frequent HR-HPV was type 33 group, and in CIN II it was as prevalent as HPV 16. The median number of copies of HR-HPV per cell was not significantly different in the CIN I, II and III cases, but there was a wide range of viral load values over several magnitudes, regardless of the grade of CIN. All samples were found to contain integrated forms of HPV 16, frequently mixed with an episomal form.

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Related in: MedlinePlus

HR-HPV viral load and CIN grade. Distribution of the log values for viral load at different CIN grades is shown. The horizontal line denotes average log values. The table contains the log as well as line values for average number of HR-HPV copies per cell at the different CIN grades.
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fig2: HR-HPV viral load and CIN grade. Distribution of the log values for viral load at different CIN grades is shown. The horizontal line denotes average log values. The table contains the log as well as line values for average number of HR-HPV copies per cell at the different CIN grades.

Mentions: The viral loads obtained for HR-HPV in the different grades of CIN are summarised in Figure 2. There was a distribution of viral load values of several orders of magnitude at all grades of CIN. The span of values of copies of HR-HPV per cell ranged from 0.14 to 8400. The mean values of the copies per cell were: CIN I – 8; CIN II – 17 and CIN III – 6. However, by using the one-way ANOVA analysis, there was no significant difference determined between these mean values.


Type distribution, viral load and integration status of high-risk human papillomaviruses in pre-stages of cervical cancer (CIN).

Andersson S, Safari H, Mints M, Lewensohn-Fuchs I, Gyllensten U, Johansson B - Br. J. Cancer (2005)

HR-HPV viral load and CIN grade. Distribution of the log values for viral load at different CIN grades is shown. The horizontal line denotes average log values. The table contains the log as well as line values for average number of HR-HPV copies per cell at the different CIN grades.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2361820&req=5

fig2: HR-HPV viral load and CIN grade. Distribution of the log values for viral load at different CIN grades is shown. The horizontal line denotes average log values. The table contains the log as well as line values for average number of HR-HPV copies per cell at the different CIN grades.
Mentions: The viral loads obtained for HR-HPV in the different grades of CIN are summarised in Figure 2. There was a distribution of viral load values of several orders of magnitude at all grades of CIN. The span of values of copies of HR-HPV per cell ranged from 0.14 to 8400. The mean values of the copies per cell were: CIN I – 8; CIN II – 17 and CIN III – 6. However, by using the one-way ANOVA analysis, there was no significant difference determined between these mean values.

Bottom Line: Two methods for the extraction of DNA from the paraffin-embedded biopsies were compared: a protocol based on the MagNA pure system (Roche) and a Qiagen spin column kit (Qiagen).HPV 16 was the most prevalent, and was found in 20, 28 and 46% of the CIN I, II and III cases, respectively.All samples were found to contain integrated forms of HPV 16, frequently mixed with an episomal form.

View Article: PubMed Central - PubMed

Affiliation: Institute for Clinical Science, Division of Obstetrics and Gynaecology, Karolinska University Hospital, Huddinge, Karolinska Institute, 141 86 Stockholm, Sweden. sonia.andersson@telia.com

ABSTRACT
A series of 176 archival cervical intraepithelial neoplasia (CIN) was analysed for the presence, viral load and integration status of 'high-risk' types of human papillomavirus (HR-HPV). The samples were assayed using newly developed methods based on real-time PCR. Two methods for the extraction of DNA from the paraffin-embedded biopsies were compared: a protocol based on the MagNA pure system (Roche) and a Qiagen spin column kit (Qiagen). It was possible to amplify 94% (166) of the samples. Of these, 36, 63 and 80% of the CIN I, II and III cases contained HR-HPV. HPV 16 was the most prevalent, and was found in 20, 28 and 46% of the CIN I, II and III cases, respectively. The second most frequent HR-HPV was type 33 group, and in CIN II it was as prevalent as HPV 16. The median number of copies of HR-HPV per cell was not significantly different in the CIN I, II and III cases, but there was a wide range of viral load values over several magnitudes, regardless of the grade of CIN. All samples were found to contain integrated forms of HPV 16, frequently mixed with an episomal form.

Show MeSH
Related in: MedlinePlus