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Reduced expression of chemokine (C-C motif) ligand-2 (CCL2) in ovarian adenocarcinoma.

Arnold JM, Huggard PR, Cummings M, Ramm GA, Chenevix-Trench G - Br. J. Cancer (2005)

Bottom Line: Chemokine (C-C motif) ligand-2 (CCL2) is a chemoattractant and activator of macrophages and is a key determinant of the macrophage infiltrate into tumours.Analysis of the CCL2 locus at 17q11.2-q12 showed loss of heterozygosity (LOH) in 70% of primary tumours, and this was significantly more common in tumours of advanced stage or grade.However, we did not detect any mutations in the CCL2 coding sequence in 94 primary ovarian adenocarcinomas.

View Article: PubMed Central - PubMed

Affiliation: The Queensland Institute of Medical Research, c/o RBH Post Office, Herston, Brisbane, QLD 4029, Australia. jeremyA@qimr.edu.au

ABSTRACT
Chemokine (C-C motif) ligand-2 (CCL2) is a chemoattractant and activator of macrophages and is a key determinant of the macrophage infiltrate into tumours. We demonstrate here that CCL2 is expressed in normal human ovarian surface epithelium (HOSE) cells and is silenced in most ovarian cancer cell lines, and silenced or downregulated in the majority of primary ovarian adenocarcinomas. Analysis of the CCL2 locus at 17q11.2-q12 showed loss of heterozygosity (LOH) in 70% of primary tumours, and this was significantly more common in tumours of advanced stage or grade. However, we did not detect any mutations in the CCL2 coding sequence in 94 primary ovarian adenocarcinomas. These data support the hypothesis that CCL2 may play a role in the pathobiology of ovarian cancers, but additional studies will be required to evaluate this possibility.

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Related in: MedlinePlus

Analysis of CCL2 expression in HOSE and ovarian adenocarcinoma cell lines by Northern blotting. Each lane represents 2 μg of polyA+ RNA. (A) Hybridisation with the CCL2 probe. (B) Hybridisation with the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe.
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fig2: Analysis of CCL2 expression in HOSE and ovarian adenocarcinoma cell lines by Northern blotting. Each lane represents 2 μg of polyA+ RNA. (A) Hybridisation with the CCL2 probe. (B) Hybridisation with the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe.

Mentions: The expression of CCL2 mRNA was next examined in a larger panel of HOSE and ovarian cancer cell lines by Northern blot analysis (Figure 2). Strong expression was detected in the HOSE17.1 and HOSE1.1 cell lines, while much weaker expression was found in the PEO14 and 27/87 cell lines. No expression was detected in the remaining 16 ovarian adenocarcinoma cell lines.


Reduced expression of chemokine (C-C motif) ligand-2 (CCL2) in ovarian adenocarcinoma.

Arnold JM, Huggard PR, Cummings M, Ramm GA, Chenevix-Trench G - Br. J. Cancer (2005)

Analysis of CCL2 expression in HOSE and ovarian adenocarcinoma cell lines by Northern blotting. Each lane represents 2 μg of polyA+ RNA. (A) Hybridisation with the CCL2 probe. (B) Hybridisation with the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2361786&req=5

fig2: Analysis of CCL2 expression in HOSE and ovarian adenocarcinoma cell lines by Northern blotting. Each lane represents 2 μg of polyA+ RNA. (A) Hybridisation with the CCL2 probe. (B) Hybridisation with the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe.
Mentions: The expression of CCL2 mRNA was next examined in a larger panel of HOSE and ovarian cancer cell lines by Northern blot analysis (Figure 2). Strong expression was detected in the HOSE17.1 and HOSE1.1 cell lines, while much weaker expression was found in the PEO14 and 27/87 cell lines. No expression was detected in the remaining 16 ovarian adenocarcinoma cell lines.

Bottom Line: Chemokine (C-C motif) ligand-2 (CCL2) is a chemoattractant and activator of macrophages and is a key determinant of the macrophage infiltrate into tumours.Analysis of the CCL2 locus at 17q11.2-q12 showed loss of heterozygosity (LOH) in 70% of primary tumours, and this was significantly more common in tumours of advanced stage or grade.However, we did not detect any mutations in the CCL2 coding sequence in 94 primary ovarian adenocarcinomas.

View Article: PubMed Central - PubMed

Affiliation: The Queensland Institute of Medical Research, c/o RBH Post Office, Herston, Brisbane, QLD 4029, Australia. jeremyA@qimr.edu.au

ABSTRACT
Chemokine (C-C motif) ligand-2 (CCL2) is a chemoattractant and activator of macrophages and is a key determinant of the macrophage infiltrate into tumours. We demonstrate here that CCL2 is expressed in normal human ovarian surface epithelium (HOSE) cells and is silenced in most ovarian cancer cell lines, and silenced or downregulated in the majority of primary ovarian adenocarcinomas. Analysis of the CCL2 locus at 17q11.2-q12 showed loss of heterozygosity (LOH) in 70% of primary tumours, and this was significantly more common in tumours of advanced stage or grade. However, we did not detect any mutations in the CCL2 coding sequence in 94 primary ovarian adenocarcinomas. These data support the hypothesis that CCL2 may play a role in the pathobiology of ovarian cancers, but additional studies will be required to evaluate this possibility.

Show MeSH
Related in: MedlinePlus