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CD133 expression is correlated with lymph node metastasis and vascular endothelial growth factor-C expression in pancreatic cancer.

Maeda S, Shinchi H, Kurahara H, Mataki Y, Maemura K, Sato M, Natsugoe S, Aikou T, Takao S - Br. J. Cancer (2008)

Bottom Line: CD133-positive cells were found at the peripheral site of adenocarcinoma glandular structures and were negative for CK.Consequently, the 5-year survival rate of CD133-positive patients was significantly lower than that of CD133-negative patients (P=0.0002) and multivariate analysis revealed that CD133 expression was an independent prognostic factor (P=0.0103).These results suggest that CD133 expression in pancreatic cancer was significantly associated with lymphatic metastasis, VEGF-C expression, and prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgical Oncology and Digestive Surgery, Field of Oncology, Course of Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Science, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan.

ABSTRACT
Although CD133 has been shown to be a marker for cancer stem cells in various tumours, its expression in pancreatic cancer has not yet been clinically reported. In this study, we investigated the relationship between CD133 expression and clinicopathological factors in pancreatic cancer. Pancreatic head carcinoma specimens from 80 patients who underwent surgical resection were immunohistochemically assessed for CD133, vascular endothelial growth factor (VEGF)-C, CXCR4, CD34, Ki-67, and cytokeratin (CK) expressions. Sixty percentage (48/80) of specimens were CD133-positive, with less than 15% cells per specimen expressing the marker. CD133-positive cells were found at the peripheral site of adenocarcinoma glandular structures and were negative for CK. There was a significant correlation between CD133 expression and clinicopathological factors, including histological type, lymphatic invasion, and lymph node metastasis (P=0.0215, 0.0023, and 0.0024, respectively). Vascular endothelial growth factor-C expression was also significantly correlated with CD133 expression (P=0.0002). Consequently, the 5-year survival rate of CD133-positive patients was significantly lower than that of CD133-negative patients (P=0.0002) and multivariate analysis revealed that CD133 expression was an independent prognostic factor (P=0.0103). These results suggest that CD133 expression in pancreatic cancer was significantly associated with lymphatic metastasis, VEGF-C expression, and prognosis.

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Classification of CD133-positive expression in the invasive ductal adenocarcinoma of the pancreas head samples from 80 patients. Tumour samples were classified into five groups (negative (0%), <5, 5–10, 11–15, and >15%) based on the percentage of stained cells.
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fig3: Classification of CD133-positive expression in the invasive ductal adenocarcinoma of the pancreas head samples from 80 patients. Tumour samples were classified into five groups (negative (0%), <5, 5–10, 11–15, and >15%) based on the percentage of stained cells.

Mentions: CD133 expression was classified into five levels: negative (0%), less than 5, 5–10, 11–15, and more than 15% (Figure 3). Forty-eight (60.0%) of 80 specimens were CD133-positive and the percentage of CD133-positive cells per specimen was less than 15% (Figure 3).


CD133 expression is correlated with lymph node metastasis and vascular endothelial growth factor-C expression in pancreatic cancer.

Maeda S, Shinchi H, Kurahara H, Mataki Y, Maemura K, Sato M, Natsugoe S, Aikou T, Takao S - Br. J. Cancer (2008)

Classification of CD133-positive expression in the invasive ductal adenocarcinoma of the pancreas head samples from 80 patients. Tumour samples were classified into five groups (negative (0%), <5, 5–10, 11–15, and >15%) based on the percentage of stained cells.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2361715&req=5

fig3: Classification of CD133-positive expression in the invasive ductal adenocarcinoma of the pancreas head samples from 80 patients. Tumour samples were classified into five groups (negative (0%), <5, 5–10, 11–15, and >15%) based on the percentage of stained cells.
Mentions: CD133 expression was classified into five levels: negative (0%), less than 5, 5–10, 11–15, and more than 15% (Figure 3). Forty-eight (60.0%) of 80 specimens were CD133-positive and the percentage of CD133-positive cells per specimen was less than 15% (Figure 3).

Bottom Line: CD133-positive cells were found at the peripheral site of adenocarcinoma glandular structures and were negative for CK.Consequently, the 5-year survival rate of CD133-positive patients was significantly lower than that of CD133-negative patients (P=0.0002) and multivariate analysis revealed that CD133 expression was an independent prognostic factor (P=0.0103).These results suggest that CD133 expression in pancreatic cancer was significantly associated with lymphatic metastasis, VEGF-C expression, and prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgical Oncology and Digestive Surgery, Field of Oncology, Course of Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Science, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan.

ABSTRACT
Although CD133 has been shown to be a marker for cancer stem cells in various tumours, its expression in pancreatic cancer has not yet been clinically reported. In this study, we investigated the relationship between CD133 expression and clinicopathological factors in pancreatic cancer. Pancreatic head carcinoma specimens from 80 patients who underwent surgical resection were immunohistochemically assessed for CD133, vascular endothelial growth factor (VEGF)-C, CXCR4, CD34, Ki-67, and cytokeratin (CK) expressions. Sixty percentage (48/80) of specimens were CD133-positive, with less than 15% cells per specimen expressing the marker. CD133-positive cells were found at the peripheral site of adenocarcinoma glandular structures and were negative for CK. There was a significant correlation between CD133 expression and clinicopathological factors, including histological type, lymphatic invasion, and lymph node metastasis (P=0.0215, 0.0023, and 0.0024, respectively). Vascular endothelial growth factor-C expression was also significantly correlated with CD133 expression (P=0.0002). Consequently, the 5-year survival rate of CD133-positive patients was significantly lower than that of CD133-negative patients (P=0.0002) and multivariate analysis revealed that CD133 expression was an independent prognostic factor (P=0.0103). These results suggest that CD133 expression in pancreatic cancer was significantly associated with lymphatic metastasis, VEGF-C expression, and prognosis.

Show MeSH
Related in: MedlinePlus