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Gene expression profiling of noninvasive primary urothelial tumours using microarrays.

Aaboe M, Marcussen N, Jensen KM, Thykjaer T, Dyrskjøt L, Orntoft TF - Br. J. Cancer (2005)

Bottom Line: At present, the mechanism leading to bladder cancer is still poorly understood, and our knowledge about early events in tumorigenesis is limited.Among the mostly changed genes between normal bladder and Ta tumours, we found genes related to the cytoskeleton (keratin 7 and syndecan 1), and transcription (high mobility group AT-hook 1).These could be detected in urine sediments from bladder tumour patients.

View Article: PubMed Central - PubMed

Affiliation: Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital Skejby, 8200 Aarhus N, Denmark.

ABSTRACT
At present, the mechanism leading to bladder cancer is still poorly understood, and our knowledge about early events in tumorigenesis is limited. This study describes the changes in gene expression occurring during the neoplastic transition from normal bladder urothelium to primary Ta tumours. Using DNA microarrays, we identified novel differentially expressed genes in Ta tumours compared to normal bladder, and genes that were altered in high-grade tumours. Among the mostly changed genes between normal bladder and Ta tumours, we found genes related to the cytoskeleton (keratin 7 and syndecan 1), and transcription (high mobility group AT-hook 1). Altered genes in high-grade tumours were related to cell cycle (cyclin-dependent kinase 4) and transcription (jun d proto-oncogene). Furthermore, we showed the presence of high keratin 7 transcript expression in bladder cancer, and Western blotting analysis revealed three major molecular isoforms of keratin 7 in the tissues. These could be detected in urine sediments from bladder tumour patients.

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Related in: MedlinePlus

Expression of KRT7 transcript in normal bladder biopsies, Ta, and T2-4 tumours (N=81) measured by microarray analysis. Median expression levels for each group are represented by horizontal lines.
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fig4: Expression of KRT7 transcript in normal bladder biopsies, Ta, and T2-4 tumours (N=81) measured by microarray analysis. Median expression levels for each group are represented by horizontal lines.

Mentions: From the gene expression analysis we found the KRT7 transcript increased by 19.8-fold in primary Ta tumours compared to normal bladder biopsies (Table 1). In order to evaluate the diagnostic value of KRT7 in bladder cancer, we looked at KRT7 expression in an expanded data set (evaluation data set 2) containing also muscle-invasive T2-4 tumours. Again, we found strong KRT7 transcript upregulation in Ta tumours (N=48), as well as in T2-4 tumours (N=24) compared to normal bladder biopsies (Figure 4). The median KRT7 expression in normal bladder biopsies was estimated to be 140.0±189.5, and in the cases of Ta and T2-4 tumours, 2775.1±1165.4 and 1192.7±698.7, respectively. This gives fold changes equal to 19.8-fold (P<0.05) and 8.5-fold (P<0.05), respectively.


Gene expression profiling of noninvasive primary urothelial tumours using microarrays.

Aaboe M, Marcussen N, Jensen KM, Thykjaer T, Dyrskjøt L, Orntoft TF - Br. J. Cancer (2005)

Expression of KRT7 transcript in normal bladder biopsies, Ta, and T2-4 tumours (N=81) measured by microarray analysis. Median expression levels for each group are represented by horizontal lines.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2361501&req=5

fig4: Expression of KRT7 transcript in normal bladder biopsies, Ta, and T2-4 tumours (N=81) measured by microarray analysis. Median expression levels for each group are represented by horizontal lines.
Mentions: From the gene expression analysis we found the KRT7 transcript increased by 19.8-fold in primary Ta tumours compared to normal bladder biopsies (Table 1). In order to evaluate the diagnostic value of KRT7 in bladder cancer, we looked at KRT7 expression in an expanded data set (evaluation data set 2) containing also muscle-invasive T2-4 tumours. Again, we found strong KRT7 transcript upregulation in Ta tumours (N=48), as well as in T2-4 tumours (N=24) compared to normal bladder biopsies (Figure 4). The median KRT7 expression in normal bladder biopsies was estimated to be 140.0±189.5, and in the cases of Ta and T2-4 tumours, 2775.1±1165.4 and 1192.7±698.7, respectively. This gives fold changes equal to 19.8-fold (P<0.05) and 8.5-fold (P<0.05), respectively.

Bottom Line: At present, the mechanism leading to bladder cancer is still poorly understood, and our knowledge about early events in tumorigenesis is limited.Among the mostly changed genes between normal bladder and Ta tumours, we found genes related to the cytoskeleton (keratin 7 and syndecan 1), and transcription (high mobility group AT-hook 1).These could be detected in urine sediments from bladder tumour patients.

View Article: PubMed Central - PubMed

Affiliation: Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital Skejby, 8200 Aarhus N, Denmark.

ABSTRACT
At present, the mechanism leading to bladder cancer is still poorly understood, and our knowledge about early events in tumorigenesis is limited. This study describes the changes in gene expression occurring during the neoplastic transition from normal bladder urothelium to primary Ta tumours. Using DNA microarrays, we identified novel differentially expressed genes in Ta tumours compared to normal bladder, and genes that were altered in high-grade tumours. Among the mostly changed genes between normal bladder and Ta tumours, we found genes related to the cytoskeleton (keratin 7 and syndecan 1), and transcription (high mobility group AT-hook 1). Altered genes in high-grade tumours were related to cell cycle (cyclin-dependent kinase 4) and transcription (jun d proto-oncogene). Furthermore, we showed the presence of high keratin 7 transcript expression in bladder cancer, and Western blotting analysis revealed three major molecular isoforms of keratin 7 in the tissues. These could be detected in urine sediments from bladder tumour patients.

Show MeSH
Related in: MedlinePlus