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Characterisation and protein expression profiling of annexins in colorectal cancer.

Duncan R, Carpenter B, Main LC, Telfer C, Murray GI - Br. J. Cancer (2007)

Bottom Line: There was significant increase in expression in annexins A1 (P=0.01), A2 (P<0.001), A4 (P<0.001) and A11 (P<0.001) in primary tumours compared with normal colon.An annexin expression profile was identified by k-means cluster analysis, and the annexin profile was associated with tumour stage (P=0.01) and also patient survival.Patients in annexin cluster group 1 (low annexin expression) had a better survival (log rank=5.33, P=0.02) than patients in cluster group 2 (high annexins A4 and A11 expression).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Aberdeen, Aberdeen, UK.

ABSTRACT
The annexins are family of calcium-regulated phospholipid-binding proteins with diverse roles in cell biology. Individual annexins have been implicated in tumour development and progression, and in this investigation a range of annexins have been studied in colorectal cancer. Annexins A1, A2, A4 and A11 were identified by comparative proteomic analysis to be overexpressed in colorectal cancer. Annexins A1, A2, A4 and A11 were further studied by immunohistochemistry with a colorectal cancer tissue microarray containing primary and metastatic colorectal cancer and also normal colon. There was significant increase in expression in annexins A1 (P=0.01), A2 (P<0.001), A4 (P<0.001) and A11 (P<0.001) in primary tumours compared with normal colon. There was increasing expression of annexins A2 (P=0.001), A4 (P=0.03) and A11 (P=0.006) with increasing tumour stage. An annexin expression profile was identified by k-means cluster analysis, and the annexin profile was associated with tumour stage (P=0.01) and also patient survival. Patients in annexin cluster group 1 (low annexin expression) had a better survival (log rank=5.33, P=0.02) than patients in cluster group 2 (high annexins A4 and A11 expression). In conclusion, this study has shown that individual annexins are present in colorectal cancer, specific annexins are overexpressed in colorectal cancer and the annexin expression profile is associated with survival.

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Comparison of the mean intensity of annexin expression in Dukes C primary tumours and corresponding lymph node metastasis.
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fig4: Comparison of the mean intensity of annexin expression in Dukes C primary tumours and corresponding lymph node metastasis.

Mentions: The annexins except annexin A7 showed immunoreactivity in lymph node metastasis. Annexins A2 and A11 showed the greatest percentage of weak staining at 20.7 and 29%, respectively, with less tumours showing moderate and strong staining for these annexins. As in normal and primary tumours, annexin A4 showed the greatest percentage of strong staining at 62.3% of tumours. Comparing the expression of annexins in lymph node metastasis with the corresponding primary tumours showed that there was a significant decrease in expression of annexin A11 (P=0.01) in lymph node metastasis compared with corresponding primary colorectal cancers (Figure 4).


Characterisation and protein expression profiling of annexins in colorectal cancer.

Duncan R, Carpenter B, Main LC, Telfer C, Murray GI - Br. J. Cancer (2007)

Comparison of the mean intensity of annexin expression in Dukes C primary tumours and corresponding lymph node metastasis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2361450&req=5

fig4: Comparison of the mean intensity of annexin expression in Dukes C primary tumours and corresponding lymph node metastasis.
Mentions: The annexins except annexin A7 showed immunoreactivity in lymph node metastasis. Annexins A2 and A11 showed the greatest percentage of weak staining at 20.7 and 29%, respectively, with less tumours showing moderate and strong staining for these annexins. As in normal and primary tumours, annexin A4 showed the greatest percentage of strong staining at 62.3% of tumours. Comparing the expression of annexins in lymph node metastasis with the corresponding primary tumours showed that there was a significant decrease in expression of annexin A11 (P=0.01) in lymph node metastasis compared with corresponding primary colorectal cancers (Figure 4).

Bottom Line: There was significant increase in expression in annexins A1 (P=0.01), A2 (P<0.001), A4 (P<0.001) and A11 (P<0.001) in primary tumours compared with normal colon.An annexin expression profile was identified by k-means cluster analysis, and the annexin profile was associated with tumour stage (P=0.01) and also patient survival.Patients in annexin cluster group 1 (low annexin expression) had a better survival (log rank=5.33, P=0.02) than patients in cluster group 2 (high annexins A4 and A11 expression).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Aberdeen, Aberdeen, UK.

ABSTRACT
The annexins are family of calcium-regulated phospholipid-binding proteins with diverse roles in cell biology. Individual annexins have been implicated in tumour development and progression, and in this investigation a range of annexins have been studied in colorectal cancer. Annexins A1, A2, A4 and A11 were identified by comparative proteomic analysis to be overexpressed in colorectal cancer. Annexins A1, A2, A4 and A11 were further studied by immunohistochemistry with a colorectal cancer tissue microarray containing primary and metastatic colorectal cancer and also normal colon. There was significant increase in expression in annexins A1 (P=0.01), A2 (P<0.001), A4 (P<0.001) and A11 (P<0.001) in primary tumours compared with normal colon. There was increasing expression of annexins A2 (P=0.001), A4 (P=0.03) and A11 (P=0.006) with increasing tumour stage. An annexin expression profile was identified by k-means cluster analysis, and the annexin profile was associated with tumour stage (P=0.01) and also patient survival. Patients in annexin cluster group 1 (low annexin expression) had a better survival (log rank=5.33, P=0.02) than patients in cluster group 2 (high annexins A4 and A11 expression). In conclusion, this study has shown that individual annexins are present in colorectal cancer, specific annexins are overexpressed in colorectal cancer and the annexin expression profile is associated with survival.

Show MeSH
Related in: MedlinePlus