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Mycosis fungoides: is it a Borrelia burgdorferi-associated disease?

Tothova SM, Bonin S, Trevisan G, Stanta G - Br. J. Cancer (2006)

Bottom Line: Mycosis fungoides (MF) is the most frequently found cutaneous T-cell lymphoma with an unknown aetiology.We looked for evidence of Borrelia burgdorferi (Bb), the aetiologic agent of Lyme disease (LD), in a case study of MF patients from Northeastern Italy, an area with endemic LD.The Bb positivity rates detected in this study support a possible role for Bb in the aetiopathogenesis of MF in a population endemic for LD.

View Article: PubMed Central - PubMed

Affiliation: ICGEB - International Centre for Genetic Engineering and Biotechnology, 99 Padriciano, Trieste 34012, Italy.

ABSTRACT
Mycosis fungoides (MF) is the most frequently found cutaneous T-cell lymphoma with an unknown aetiology. Several aetiopathogenetic mechanisms have been postulated, including persistent viral or bacterial infections. We looked for evidence of Borrelia burgdorferi (Bb), the aetiologic agent of Lyme disease (LD), in a case study of MF patients from Northeastern Italy, an area with endemic LD. Polymerase chain reaction for the flagellin gene of Bb was used to study formalin-fixed paraffin-embedded lesional skin biopsies from 83 patients with MF and 83 sex- and age-matched healthy controls with homolocalised cutaneous nevi. Borrelia burgdorferi-specific sequence was detected in 15 out of 83 skin samples of patients with MF (18.1%), but in none out of 83 matched healthy controls (P<0.0001). The Bb positivity rates detected in this study support a possible role for Bb in the aetiopathogenesis of MF in a population endemic for LD.

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Related in: MedlinePlus

Examples of polymerase-chain reaction analysis of T-cell receptor-γ gene rearrangements. Lanes 1, 3 and 5 are MF samples positive for the rearrangements; lanes 2 and 4 are MF cases negative for the rearrangements. Lane 6 is molecular marker ladder.
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fig2: Examples of polymerase-chain reaction analysis of T-cell receptor-γ gene rearrangements. Lanes 1, 3 and 5 are MF samples positive for the rearrangements; lanes 2 and 4 are MF cases negative for the rearrangements. Lane 6 is molecular marker ladder.

Mentions: In cases positive for the Bb genome, we analysed the TCR-γ gene rearrangements using PCR analysis. A monoclonal T-cell infiltrate was demonstrated in 12 out of 15 Bb positive cases (80%). In 10 cases, one band in the range of 80–85 bases was observed and in two cases two bands in the same range were found representing a biallelic rearrangement of the TCR-γ genes (Figure 2) (McCarthy et al, 1992).


Mycosis fungoides: is it a Borrelia burgdorferi-associated disease?

Tothova SM, Bonin S, Trevisan G, Stanta G - Br. J. Cancer (2006)

Examples of polymerase-chain reaction analysis of T-cell receptor-γ gene rearrangements. Lanes 1, 3 and 5 are MF samples positive for the rearrangements; lanes 2 and 4 are MF cases negative for the rearrangements. Lane 6 is molecular marker ladder.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2361364&req=5

fig2: Examples of polymerase-chain reaction analysis of T-cell receptor-γ gene rearrangements. Lanes 1, 3 and 5 are MF samples positive for the rearrangements; lanes 2 and 4 are MF cases negative for the rearrangements. Lane 6 is molecular marker ladder.
Mentions: In cases positive for the Bb genome, we analysed the TCR-γ gene rearrangements using PCR analysis. A monoclonal T-cell infiltrate was demonstrated in 12 out of 15 Bb positive cases (80%). In 10 cases, one band in the range of 80–85 bases was observed and in two cases two bands in the same range were found representing a biallelic rearrangement of the TCR-γ genes (Figure 2) (McCarthy et al, 1992).

Bottom Line: Mycosis fungoides (MF) is the most frequently found cutaneous T-cell lymphoma with an unknown aetiology.We looked for evidence of Borrelia burgdorferi (Bb), the aetiologic agent of Lyme disease (LD), in a case study of MF patients from Northeastern Italy, an area with endemic LD.The Bb positivity rates detected in this study support a possible role for Bb in the aetiopathogenesis of MF in a population endemic for LD.

View Article: PubMed Central - PubMed

Affiliation: ICGEB - International Centre for Genetic Engineering and Biotechnology, 99 Padriciano, Trieste 34012, Italy.

ABSTRACT
Mycosis fungoides (MF) is the most frequently found cutaneous T-cell lymphoma with an unknown aetiology. Several aetiopathogenetic mechanisms have been postulated, including persistent viral or bacterial infections. We looked for evidence of Borrelia burgdorferi (Bb), the aetiologic agent of Lyme disease (LD), in a case study of MF patients from Northeastern Italy, an area with endemic LD. Polymerase chain reaction for the flagellin gene of Bb was used to study formalin-fixed paraffin-embedded lesional skin biopsies from 83 patients with MF and 83 sex- and age-matched healthy controls with homolocalised cutaneous nevi. Borrelia burgdorferi-specific sequence was detected in 15 out of 83 skin samples of patients with MF (18.1%), but in none out of 83 matched healthy controls (P<0.0001). The Bb positivity rates detected in this study support a possible role for Bb in the aetiopathogenesis of MF in a population endemic for LD.

Show MeSH
Related in: MedlinePlus