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Epidermal stem cells are retained in vivo throughout skin aging.

Giangreco A, Qin M, Pintar JE, Watt FM - Aging Cell (2008)

Bottom Line: In healthy individuals, skin integrity is maintained by epidermal stem cells which self-renew and generate daughter cells that undergo terminal differentiation.It is currently unknown whether epidermal stem cells influence or are affected by skin aging.We therefore compared young and aged skin stem cell abundance, organization, and proliferation.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK. adam.giangreco@cancer.org.uk

ABSTRACT
In healthy individuals, skin integrity is maintained by epidermal stem cells which self-renew and generate daughter cells that undergo terminal differentiation. It is currently unknown whether epidermal stem cells influence or are affected by skin aging. We therefore compared young and aged skin stem cell abundance, organization, and proliferation. We discovered that despite age-associated differences in epidermal proliferation, dermal thickness, follicle patterning, and immune cell abundance, epidermal stem cells were maintained at normal levels throughout life. These findings, coupled with observed dermal gene expression changes, suggest that epidermal stem cells themselves are intrinsically aging resistant and that local environmental or systemic factors modulate skin aging.

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Aging results in altered skin leukocyte abundance. (A, B) Representative whole mounts from young (A) and old (B) tail epidermis stained for the pan-haematopoietic marker CD45 (red) and the keratinocyte-specific marker keratin 14 (green). (C) Quantification of CD45(+) cells present per square millimetre of tissue. (D, E) Young (D) and old (E) epidermal whole mounts stained to reveal haematopoietic Langerhans cells (DEC205, red). (F) Quantification of DEC205(+) cells per square millimetre of epidermis. (G, H) Whole-mount immunostaining for T cell populations using antibodies to the pan-T cell marker CD3 (red) and γδ-T cell receptor (γδ-TCR; green) to identify dendritic epidermal T cells (DETCs, orange dual stain). (I) Quantification of CD3 (red) and γδTCR/CD3 dual (green) positive cells per square millimetre of epidermis. All images and quantification represent at least n = 3 individuals/age. Scale bars = 200 µm (A, B); 100 µm (C–F).
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fig04: Aging results in altered skin leukocyte abundance. (A, B) Representative whole mounts from young (A) and old (B) tail epidermis stained for the pan-haematopoietic marker CD45 (red) and the keratinocyte-specific marker keratin 14 (green). (C) Quantification of CD45(+) cells present per square millimetre of tissue. (D, E) Young (D) and old (E) epidermal whole mounts stained to reveal haematopoietic Langerhans cells (DEC205, red). (F) Quantification of DEC205(+) cells per square millimetre of epidermis. (G, H) Whole-mount immunostaining for T cell populations using antibodies to the pan-T cell marker CD3 (red) and γδ-T cell receptor (γδ-TCR; green) to identify dendritic epidermal T cells (DETCs, orange dual stain). (I) Quantification of CD3 (red) and γδTCR/CD3 dual (green) positive cells per square millimetre of epidermis. All images and quantification represent at least n = 3 individuals/age. Scale bars = 200 µm (A, B); 100 µm (C–F).

Mentions: In addition to altered morphology, skin aging is frequently associated with reduced peripheral immunity and increased infection (McCullough & Kelly, 2006). To study whether aging influences murine skin immunity, epidermal whole mounts were stained for the pan-haematopoietic cell antigen CD45. There appeared to be fewer CD45 reactive cells within the IFE of old mice but this was not statistically significant (Fig. 4A–C). In young but not old mice, most of these CD45 positive cells exhibited a dendritic morphology. CD45 positive cells present in old skin were localized to the follicular–interfollicular junction (infundibulum; Fig. 4A,B).


Epidermal stem cells are retained in vivo throughout skin aging.

Giangreco A, Qin M, Pintar JE, Watt FM - Aging Cell (2008)

Aging results in altered skin leukocyte abundance. (A, B) Representative whole mounts from young (A) and old (B) tail epidermis stained for the pan-haematopoietic marker CD45 (red) and the keratinocyte-specific marker keratin 14 (green). (C) Quantification of CD45(+) cells present per square millimetre of tissue. (D, E) Young (D) and old (E) epidermal whole mounts stained to reveal haematopoietic Langerhans cells (DEC205, red). (F) Quantification of DEC205(+) cells per square millimetre of epidermis. (G, H) Whole-mount immunostaining for T cell populations using antibodies to the pan-T cell marker CD3 (red) and γδ-T cell receptor (γδ-TCR; green) to identify dendritic epidermal T cells (DETCs, orange dual stain). (I) Quantification of CD3 (red) and γδTCR/CD3 dual (green) positive cells per square millimetre of epidermis. All images and quantification represent at least n = 3 individuals/age. Scale bars = 200 µm (A, B); 100 µm (C–F).
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Related In: Results  -  Collection

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fig04: Aging results in altered skin leukocyte abundance. (A, B) Representative whole mounts from young (A) and old (B) tail epidermis stained for the pan-haematopoietic marker CD45 (red) and the keratinocyte-specific marker keratin 14 (green). (C) Quantification of CD45(+) cells present per square millimetre of tissue. (D, E) Young (D) and old (E) epidermal whole mounts stained to reveal haematopoietic Langerhans cells (DEC205, red). (F) Quantification of DEC205(+) cells per square millimetre of epidermis. (G, H) Whole-mount immunostaining for T cell populations using antibodies to the pan-T cell marker CD3 (red) and γδ-T cell receptor (γδ-TCR; green) to identify dendritic epidermal T cells (DETCs, orange dual stain). (I) Quantification of CD3 (red) and γδTCR/CD3 dual (green) positive cells per square millimetre of epidermis. All images and quantification represent at least n = 3 individuals/age. Scale bars = 200 µm (A, B); 100 µm (C–F).
Mentions: In addition to altered morphology, skin aging is frequently associated with reduced peripheral immunity and increased infection (McCullough & Kelly, 2006). To study whether aging influences murine skin immunity, epidermal whole mounts were stained for the pan-haematopoietic cell antigen CD45. There appeared to be fewer CD45 reactive cells within the IFE of old mice but this was not statistically significant (Fig. 4A–C). In young but not old mice, most of these CD45 positive cells exhibited a dendritic morphology. CD45 positive cells present in old skin were localized to the follicular–interfollicular junction (infundibulum; Fig. 4A,B).

Bottom Line: In healthy individuals, skin integrity is maintained by epidermal stem cells which self-renew and generate daughter cells that undergo terminal differentiation.It is currently unknown whether epidermal stem cells influence or are affected by skin aging.We therefore compared young and aged skin stem cell abundance, organization, and proliferation.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK. adam.giangreco@cancer.org.uk

ABSTRACT
In healthy individuals, skin integrity is maintained by epidermal stem cells which self-renew and generate daughter cells that undergo terminal differentiation. It is currently unknown whether epidermal stem cells influence or are affected by skin aging. We therefore compared young and aged skin stem cell abundance, organization, and proliferation. We discovered that despite age-associated differences in epidermal proliferation, dermal thickness, follicle patterning, and immune cell abundance, epidermal stem cells were maintained at normal levels throughout life. These findings, coupled with observed dermal gene expression changes, suggest that epidermal stem cells themselves are intrinsically aging resistant and that local environmental or systemic factors modulate skin aging.

Show MeSH
Related in: MedlinePlus