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Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice.

Fang J, Chai C, Qian Q, Li C, Tang J, Sun L, Huang Z, Guo X, Sun C, Liu M, Zhang Y, Lu Q, Wang Y, Lu C, Han B, Chen F, Cheng Z, Chu C - Plant J. (2008)

Bottom Line: Pre-harvest sprouting (PHS) or vivipary in cereals is an important agronomic trait that results in significant economic loss.As expected, the amount of ABA was reduced in all four phs mutants compared with that in the wild type.These results suggest that the impairment of carotenoid biosynthesis causes photo-oxidation and ABA-deficiency phenotypes, of which the latter is a major factor controlling the PHS trait in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research, Beijing 100101, China.

ABSTRACT
Pre-harvest sprouting (PHS) or vivipary in cereals is an important agronomic trait that results in significant economic loss. A considerable number of mutations that cause PHS have been identified in several species. However, relatively few viviparous mutants in rice (Oryza sativa L.) have been reported. To explore the mechanism of PHS in rice, we carried out an extensive genetic screening and identified 12 PHS mutants (phs). Based on their phenotypes, these phs mutants were classified into three groups. Here we characterize in detail one of these groups, which contains mutations in genes encoding major enzymes of the carotenoid biosynthesis pathway, including phytoene desaturase (OsPDS), zeta-carotene desaturase (OsZDS), carotenoid isomerase (OsCRTISO) and lycopene beta-cyclase (beta-OsLCY), which are essential for the biosynthesis of carotenoid precursors of ABA. As expected, the amount of ABA was reduced in all four phs mutants compared with that in the wild type. Chlorophyll fluorescence analysis revealed the occurrence of photoinhibition in the photosystem and decreased capacity for eliminating excess energy by thermal dissipation. The greatly increased activities of reactive oxygen species (ROS) scavenging enzymes, and reduced photosystem (PS) II core proteins CP43, CP47 and D1 in leaves of the Oscrtiso/phs3-1mutant and OsLCY RNAi transgenic rice indicated that photo-oxidative damage occurred in PS II, consistent with the accumulation of ROS in these plants. These results suggest that the impairment of carotenoid biosynthesis causes photo-oxidation and ABA-deficiency phenotypes, of which the latter is a major factor controlling the PHS trait in rice.

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Western analysis of the PS II core proteins CP47, CP43 and D1.
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fig08: Western analysis of the PS II core proteins CP47, CP43 and D1.

Mentions: To get further evidence for the photo-oxidative damage occurring in PS II, we examined the levels of essential PS II core proteins CP43, CP47 and D1 (Green and Durnford, 1996), which are the targets of ROS (Niyogi, 1999), in the leaves from the phs3-1 mutant and β-OsLCY-RNAi plants, respectively. Western analysis showed that the level of these proteins in the phs3-1 mutant and β-OsLCY-RNAi plants decreased to 64–77% of that in the wild type, indicating the occurrence of photo-oxidative damage in PS II core proteins (Figure 8).


Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice.

Fang J, Chai C, Qian Q, Li C, Tang J, Sun L, Huang Z, Guo X, Sun C, Liu M, Zhang Y, Lu Q, Wang Y, Lu C, Han B, Chen F, Cheng Z, Chu C - Plant J. (2008)

Western analysis of the PS II core proteins CP47, CP43 and D1.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2327239&req=5

fig08: Western analysis of the PS II core proteins CP47, CP43 and D1.
Mentions: To get further evidence for the photo-oxidative damage occurring in PS II, we examined the levels of essential PS II core proteins CP43, CP47 and D1 (Green and Durnford, 1996), which are the targets of ROS (Niyogi, 1999), in the leaves from the phs3-1 mutant and β-OsLCY-RNAi plants, respectively. Western analysis showed that the level of these proteins in the phs3-1 mutant and β-OsLCY-RNAi plants decreased to 64–77% of that in the wild type, indicating the occurrence of photo-oxidative damage in PS II core proteins (Figure 8).

Bottom Line: Pre-harvest sprouting (PHS) or vivipary in cereals is an important agronomic trait that results in significant economic loss.As expected, the amount of ABA was reduced in all four phs mutants compared with that in the wild type.These results suggest that the impairment of carotenoid biosynthesis causes photo-oxidation and ABA-deficiency phenotypes, of which the latter is a major factor controlling the PHS trait in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research, Beijing 100101, China.

ABSTRACT
Pre-harvest sprouting (PHS) or vivipary in cereals is an important agronomic trait that results in significant economic loss. A considerable number of mutations that cause PHS have been identified in several species. However, relatively few viviparous mutants in rice (Oryza sativa L.) have been reported. To explore the mechanism of PHS in rice, we carried out an extensive genetic screening and identified 12 PHS mutants (phs). Based on their phenotypes, these phs mutants were classified into three groups. Here we characterize in detail one of these groups, which contains mutations in genes encoding major enzymes of the carotenoid biosynthesis pathway, including phytoene desaturase (OsPDS), zeta-carotene desaturase (OsZDS), carotenoid isomerase (OsCRTISO) and lycopene beta-cyclase (beta-OsLCY), which are essential for the biosynthesis of carotenoid precursors of ABA. As expected, the amount of ABA was reduced in all four phs mutants compared with that in the wild type. Chlorophyll fluorescence analysis revealed the occurrence of photoinhibition in the photosystem and decreased capacity for eliminating excess energy by thermal dissipation. The greatly increased activities of reactive oxygen species (ROS) scavenging enzymes, and reduced photosystem (PS) II core proteins CP43, CP47 and D1 in leaves of the Oscrtiso/phs3-1mutant and OsLCY RNAi transgenic rice indicated that photo-oxidative damage occurred in PS II, consistent with the accumulation of ROS in these plants. These results suggest that the impairment of carotenoid biosynthesis causes photo-oxidation and ABA-deficiency phenotypes, of which the latter is a major factor controlling the PHS trait in rice.

Show MeSH
Related in: MedlinePlus