Limits...
Post traumatic brain perfusion SPECT analysis using reconstructed ROI maps of radioactive microsphere derived cerebral blood flow and statistical parametric mapping.

McGoron AJ, Capille M, Georgiou MF, Sanchez P, Solano J, Gonzalez-Brito M, Kuluz JW - BMC Med Imaging (2008)

Bottom Line: The purpose of this study was to establish an improved method for evaluating regional CBF changes after TBI in piglets.A significant area of hypoperfusion (P < 0.01) was found as a response to the TBI.Further study and correlation of this characteristic lesion with long-term outcomes and auxiliary diagnostic modalities is critical to developing more effective critical care treatment guidelines and automated medical imaging processing techniques.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, EC 2671, Miami, FL 33199, USA. mcgoron@fiu.edu

ABSTRACT

Background: Assessment of cerebral blood flow (CBF) by SPECT could be important in the management of patients with severe traumatic brain injury (TBI) because changes in regional CBF can affect outcome by promoting edema formation and intracranial pressure elevation (with cerebral hyperemia), or by causing secondary ischemic injury including post-traumatic stroke. The purpose of this study was to establish an improved method for evaluating regional CBF changes after TBI in piglets.

Methods: The focal effects of moderate traumatic brain injury (TBI) on cerebral blood flow (CBF) by SPECT cerebral blood perfusion (CBP) imaging in an animal model were investigated by parallelized statistical techniques. Regional CBF was measured by radioactive microspheres and by SPECT 2 hours after injury in sham-operated piglets versus those receiving severe TBI by fluid-percussion injury to the left parietal lobe. Qualitative SPECT CBP accuracy was assessed against reference radioactive microsphere regional CBF measurements by map reconstruction, registration and smoothing. Cerebral hypoperfusion in the test group was identified at the voxel level using statistical parametric mapping (SPM).

Results: A significant area of hypoperfusion (P < 0.01) was found as a response to the TBI. Statistical mapping of the reference microsphere CBF data confirms a focal decrease found with SPECT and SPM.

Conclusion: The suitability of SPM for application to the experimental model and ability to provide insight into CBF changes in response to traumatic injury was validated by the SPECT SPM result of a decrease in CBP at the left parietal region injury area of the test group. Further study and correlation of this characteristic lesion with long-term outcomes and auxiliary diagnostic modalities is critical to developing more effective critical care treatment guidelines and automated medical imaging processing techniques.

Show MeSH

Related in: MedlinePlus

Visual validation of spatial registration results was accomplished through side by side visual inspection using the SPM2 orthogonal viewer. Orthogonal views are (a) coronal (b) sagittal and (c) transverse. On the left side are the othrogonal views for subject 16. On the right side are the three orthogonal views for the template, subject 17. The brain is shown as the area of highest intensity in views (b) and (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2311288&req=5

Figure 5: Visual validation of spatial registration results was accomplished through side by side visual inspection using the SPM2 orthogonal viewer. Orthogonal views are (a) coronal (b) sagittal and (c) transverse. On the left side are the othrogonal views for subject 16. On the right side are the three orthogonal views for the template, subject 17. The brain is shown as the area of highest intensity in views (b) and (c).

Mentions: Image data preprocessing consisted of spatial normalization between all subjects with subject 17 used as a template for registration. The error minimization algorithm was set to halt matching of each subject to the template at an error threshold of 0.05. Results of spatial normalization were determined to be successful by visual inspection using the orthogonal viewer in SPM2 (Figure 5). Model specification and parameter estimation in SPM2 resulted in a map of p-values for interpretation. The threshold t-score image in Figure 6 is given by the t contrast [-1 1] testing for any statistically significant decreases in CBP in the test group. The decrease of CBP in the left parietal region is clearly shown and is the only significant change reported in the brain area. The expected location of the lesion was identified by SPM for each of the 11 test animals (p < 0.01 for each case).


Post traumatic brain perfusion SPECT analysis using reconstructed ROI maps of radioactive microsphere derived cerebral blood flow and statistical parametric mapping.

McGoron AJ, Capille M, Georgiou MF, Sanchez P, Solano J, Gonzalez-Brito M, Kuluz JW - BMC Med Imaging (2008)

Visual validation of spatial registration results was accomplished through side by side visual inspection using the SPM2 orthogonal viewer. Orthogonal views are (a) coronal (b) sagittal and (c) transverse. On the left side are the othrogonal views for subject 16. On the right side are the three orthogonal views for the template, subject 17. The brain is shown as the area of highest intensity in views (b) and (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2311288&req=5

Figure 5: Visual validation of spatial registration results was accomplished through side by side visual inspection using the SPM2 orthogonal viewer. Orthogonal views are (a) coronal (b) sagittal and (c) transverse. On the left side are the othrogonal views for subject 16. On the right side are the three orthogonal views for the template, subject 17. The brain is shown as the area of highest intensity in views (b) and (c).
Mentions: Image data preprocessing consisted of spatial normalization between all subjects with subject 17 used as a template for registration. The error minimization algorithm was set to halt matching of each subject to the template at an error threshold of 0.05. Results of spatial normalization were determined to be successful by visual inspection using the orthogonal viewer in SPM2 (Figure 5). Model specification and parameter estimation in SPM2 resulted in a map of p-values for interpretation. The threshold t-score image in Figure 6 is given by the t contrast [-1 1] testing for any statistically significant decreases in CBP in the test group. The decrease of CBP in the left parietal region is clearly shown and is the only significant change reported in the brain area. The expected location of the lesion was identified by SPM for each of the 11 test animals (p < 0.01 for each case).

Bottom Line: The purpose of this study was to establish an improved method for evaluating regional CBF changes after TBI in piglets.A significant area of hypoperfusion (P < 0.01) was found as a response to the TBI.Further study and correlation of this characteristic lesion with long-term outcomes and auxiliary diagnostic modalities is critical to developing more effective critical care treatment guidelines and automated medical imaging processing techniques.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, EC 2671, Miami, FL 33199, USA. mcgoron@fiu.edu

ABSTRACT

Background: Assessment of cerebral blood flow (CBF) by SPECT could be important in the management of patients with severe traumatic brain injury (TBI) because changes in regional CBF can affect outcome by promoting edema formation and intracranial pressure elevation (with cerebral hyperemia), or by causing secondary ischemic injury including post-traumatic stroke. The purpose of this study was to establish an improved method for evaluating regional CBF changes after TBI in piglets.

Methods: The focal effects of moderate traumatic brain injury (TBI) on cerebral blood flow (CBF) by SPECT cerebral blood perfusion (CBP) imaging in an animal model were investigated by parallelized statistical techniques. Regional CBF was measured by radioactive microspheres and by SPECT 2 hours after injury in sham-operated piglets versus those receiving severe TBI by fluid-percussion injury to the left parietal lobe. Qualitative SPECT CBP accuracy was assessed against reference radioactive microsphere regional CBF measurements by map reconstruction, registration and smoothing. Cerebral hypoperfusion in the test group was identified at the voxel level using statistical parametric mapping (SPM).

Results: A significant area of hypoperfusion (P < 0.01) was found as a response to the TBI. Statistical mapping of the reference microsphere CBF data confirms a focal decrease found with SPECT and SPM.

Conclusion: The suitability of SPM for application to the experimental model and ability to provide insight into CBF changes in response to traumatic injury was validated by the SPECT SPM result of a decrease in CBP at the left parietal region injury area of the test group. Further study and correlation of this characteristic lesion with long-term outcomes and auxiliary diagnostic modalities is critical to developing more effective critical care treatment guidelines and automated medical imaging processing techniques.

Show MeSH
Related in: MedlinePlus