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Saikokeishito Extract Exerts a Therapeutic Effect on alpha-Naphthylisothiocyanate-Induced Liver Injury in Rats through Attenuation of Enhanced Neutrophil Infiltration and Oxidative Stress in the Liver Tissue.

Ohta Y, Kongo-Nishimura M, Hayashi T, Kitagawa A, Matsura T, Yamada K - J Clin Biochem Nutr (2007)

Bottom Line: At 24 h after ANIT treatment, increases in hepatic lipid peroxide and reduced glutathione contents and myeloperoxidase activity occurred with decreases in hepatic superoxide dismutase and glutathione reductase activities.At 48 h after ANIT treatment, these changes except for reduced glutathione were enhanced with decreases in catalase, Se-glutathione peroxidase, and glucose-6-phosphate dehydrogenase activities.These results indicate that TJ-10 exerts a therapeutic effect on ANIT-induced liver injury in rats possibly through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan.

ABSTRACT
We examined whether Saikokeishito extract (TJ-10), a traditional Japanese herbal medicine, exerts a therapeutic effect on alpha-naphthylisothiocyanate (ANIT)-induced liver injury in rats through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue. In rats treated once with ANIT (75 mg/kg, i.p.), liver injury with cholestasis occurred 24 h after treatment and progressed at 48 h. When ANIT-treated rats orally received TJ-10 (0.26, 1.3 or 2.6 g/kg) at 24 h after the treatment, progressive liver injury with cholestasis was significantly attenuated at 48 h after the treatment at the dose of 1.3 or 2.6 g/kg. At 24 h after ANIT treatment, increases in hepatic lipid peroxide and reduced glutathione contents and myeloperoxidase activity occurred with decreases in hepatic superoxide dismutase and glutathione reductase activities. At 48 h after ANIT treatment, these changes except for reduced glutathione were enhanced with decreases in catalase, Se-glutathione peroxidase, and glucose-6-phosphate dehydrogenase activities. TJ-10 (1.3 or 2.6 g/kg) post-administered to ANIT-treated rats attenuated these changes found at 48 h after the treatment significantly. These results indicate that TJ-10 exerts a therapeutic effect on ANIT-induced liver injury in rats possibly through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue.

No MeSH data available.


Related in: MedlinePlus

Effect of orally post-administered TJ-10 on hepatic SOD (A) and catalase (B) activities in rats treated with and without ANIT. Experimental condition and explanation are the same as described in the legend for Fig. 2 except that hepatic SOD and catalse were assayed as described in Materials and Methods.
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Figure 6: Effect of orally post-administered TJ-10 on hepatic SOD (A) and catalase (B) activities in rats treated with and without ANIT. Experimental condition and explanation are the same as described in the legend for Fig. 2 except that hepatic SOD and catalse were assayed as described in Materials and Methods.

Mentions: Hepatic SOD and GSSG-R activities in the ANIT-treated group were significantly lower than those in the control group at 24 h after treatment, while there were no significant differences in hepatic catalase, Se-GSHpx, and G-6-PDH activities between the ANIT-treated and untreated control rats at 24 h (Figs. 6 and 7). In the ANIT-treated group, further decreases in hepatic SOD and GSSG-R activities occurred with decreases in hepatic catalase, Se-GSHpx, and G-6-PDH activities at 48 h after the treatment (Figs. 6 and 7). TJ-10 post-administered to ANIT-treated rats at a dose of 1.3 or 2.6 g/kg BW, but not 0.26 g/kg BW, significantly reduced the enhanced decreases in hepatic SOD and GSSG-R activities and the decreases in hepatic catalase, Se-GSHpx, and G-6-PDH activities at 48 h after the treatment (Figs. 6 and 7). The hepatic SOD activity in the ANIT-treated group given TJ-10 (1.3 or 2.6 g/kg BW) was not significantly different from that in the ANIT-treated group found at 24 h after the treatment (Fig. 6A) In addition, the ANIT-treated group given TJ-10 (2.6 g/kg BW) had as much hepatic G-6-PDH activity as the control group (Fig. 7C). The same doses of TJ-10 given to ANIT-untreated rats did not affect the hepatic SOD, GSSG-R, catalase, and Se-GSHpx activities but the hepatic G-6-PDH activity in ANIT-untreated group given TJ-10 at a dose of 2.6 g/kg BW, but not 0.26 or 1.3 g/kg BW, was significantly higher than that in untreated control rats (Figs. 6 and 7).


Saikokeishito Extract Exerts a Therapeutic Effect on alpha-Naphthylisothiocyanate-Induced Liver Injury in Rats through Attenuation of Enhanced Neutrophil Infiltration and Oxidative Stress in the Liver Tissue.

Ohta Y, Kongo-Nishimura M, Hayashi T, Kitagawa A, Matsura T, Yamada K - J Clin Biochem Nutr (2007)

Effect of orally post-administered TJ-10 on hepatic SOD (A) and catalase (B) activities in rats treated with and without ANIT. Experimental condition and explanation are the same as described in the legend for Fig. 2 except that hepatic SOD and catalse were assayed as described in Materials and Methods.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2291502&req=5

Figure 6: Effect of orally post-administered TJ-10 on hepatic SOD (A) and catalase (B) activities in rats treated with and without ANIT. Experimental condition and explanation are the same as described in the legend for Fig. 2 except that hepatic SOD and catalse were assayed as described in Materials and Methods.
Mentions: Hepatic SOD and GSSG-R activities in the ANIT-treated group were significantly lower than those in the control group at 24 h after treatment, while there were no significant differences in hepatic catalase, Se-GSHpx, and G-6-PDH activities between the ANIT-treated and untreated control rats at 24 h (Figs. 6 and 7). In the ANIT-treated group, further decreases in hepatic SOD and GSSG-R activities occurred with decreases in hepatic catalase, Se-GSHpx, and G-6-PDH activities at 48 h after the treatment (Figs. 6 and 7). TJ-10 post-administered to ANIT-treated rats at a dose of 1.3 or 2.6 g/kg BW, but not 0.26 g/kg BW, significantly reduced the enhanced decreases in hepatic SOD and GSSG-R activities and the decreases in hepatic catalase, Se-GSHpx, and G-6-PDH activities at 48 h after the treatment (Figs. 6 and 7). The hepatic SOD activity in the ANIT-treated group given TJ-10 (1.3 or 2.6 g/kg BW) was not significantly different from that in the ANIT-treated group found at 24 h after the treatment (Fig. 6A) In addition, the ANIT-treated group given TJ-10 (2.6 g/kg BW) had as much hepatic G-6-PDH activity as the control group (Fig. 7C). The same doses of TJ-10 given to ANIT-untreated rats did not affect the hepatic SOD, GSSG-R, catalase, and Se-GSHpx activities but the hepatic G-6-PDH activity in ANIT-untreated group given TJ-10 at a dose of 2.6 g/kg BW, but not 0.26 or 1.3 g/kg BW, was significantly higher than that in untreated control rats (Figs. 6 and 7).

Bottom Line: At 24 h after ANIT treatment, increases in hepatic lipid peroxide and reduced glutathione contents and myeloperoxidase activity occurred with decreases in hepatic superoxide dismutase and glutathione reductase activities.At 48 h after ANIT treatment, these changes except for reduced glutathione were enhanced with decreases in catalase, Se-glutathione peroxidase, and glucose-6-phosphate dehydrogenase activities.These results indicate that TJ-10 exerts a therapeutic effect on ANIT-induced liver injury in rats possibly through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan.

ABSTRACT
We examined whether Saikokeishito extract (TJ-10), a traditional Japanese herbal medicine, exerts a therapeutic effect on alpha-naphthylisothiocyanate (ANIT)-induced liver injury in rats through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue. In rats treated once with ANIT (75 mg/kg, i.p.), liver injury with cholestasis occurred 24 h after treatment and progressed at 48 h. When ANIT-treated rats orally received TJ-10 (0.26, 1.3 or 2.6 g/kg) at 24 h after the treatment, progressive liver injury with cholestasis was significantly attenuated at 48 h after the treatment at the dose of 1.3 or 2.6 g/kg. At 24 h after ANIT treatment, increases in hepatic lipid peroxide and reduced glutathione contents and myeloperoxidase activity occurred with decreases in hepatic superoxide dismutase and glutathione reductase activities. At 48 h after ANIT treatment, these changes except for reduced glutathione were enhanced with decreases in catalase, Se-glutathione peroxidase, and glucose-6-phosphate dehydrogenase activities. TJ-10 (1.3 or 2.6 g/kg) post-administered to ANIT-treated rats attenuated these changes found at 48 h after the treatment significantly. These results indicate that TJ-10 exerts a therapeutic effect on ANIT-induced liver injury in rats possibly through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue.

No MeSH data available.


Related in: MedlinePlus