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Saikokeishito Extract Exerts a Therapeutic Effect on alpha-Naphthylisothiocyanate-Induced Liver Injury in Rats through Attenuation of Enhanced Neutrophil Infiltration and Oxidative Stress in the Liver Tissue.

Ohta Y, Kongo-Nishimura M, Hayashi T, Kitagawa A, Matsura T, Yamada K - J Clin Biochem Nutr (2007)

Bottom Line: At 24 h after ANIT treatment, increases in hepatic lipid peroxide and reduced glutathione contents and myeloperoxidase activity occurred with decreases in hepatic superoxide dismutase and glutathione reductase activities.At 48 h after ANIT treatment, these changes except for reduced glutathione were enhanced with decreases in catalase, Se-glutathione peroxidase, and glucose-6-phosphate dehydrogenase activities.These results indicate that TJ-10 exerts a therapeutic effect on ANIT-induced liver injury in rats possibly through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan.

ABSTRACT
We examined whether Saikokeishito extract (TJ-10), a traditional Japanese herbal medicine, exerts a therapeutic effect on alpha-naphthylisothiocyanate (ANIT)-induced liver injury in rats through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue. In rats treated once with ANIT (75 mg/kg, i.p.), liver injury with cholestasis occurred 24 h after treatment and progressed at 48 h. When ANIT-treated rats orally received TJ-10 (0.26, 1.3 or 2.6 g/kg) at 24 h after the treatment, progressive liver injury with cholestasis was significantly attenuated at 48 h after the treatment at the dose of 1.3 or 2.6 g/kg. At 24 h after ANIT treatment, increases in hepatic lipid peroxide and reduced glutathione contents and myeloperoxidase activity occurred with decreases in hepatic superoxide dismutase and glutathione reductase activities. At 48 h after ANIT treatment, these changes except for reduced glutathione were enhanced with decreases in catalase, Se-glutathione peroxidase, and glucose-6-phosphate dehydrogenase activities. TJ-10 (1.3 or 2.6 g/kg) post-administered to ANIT-treated rats attenuated these changes found at 48 h after the treatment significantly. These results indicate that TJ-10 exerts a therapeutic effect on ANIT-induced liver injury in rats possibly through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue.

No MeSH data available.


Related in: MedlinePlus

Effect of orally post-Administered TJ-10 on serum AST (A), ALT (B), and γ-GTP (C) activities and total blirubin concentration (D) in rats treated with and without ANIT. TJ-10 (0.26, 1.3 or 2.6 g/kg BW) was orally administered to rats treated with and without ANIT (75 mg/kg BW, i.p.) 24 h after the treatment. Rats not given TJ-10 received vehicle at the same time point. AST, ALT, γ-GTP, and total bilirubin in the serum of each rat were assayed 24 or 48 h after ANIT treatment as described in Materials and Methods. Each value is a mean ± S.D. (n = 5 for ANIT-untreated rats with and without TJ-10 administration; n = 8 for ANIT-treated rats with and without TJ-10 administration). *Significantly different from control rats without any treatment, p<0.05. #Significantly different from ANIT-treated rats without TJ-10 administration, p<0.05.
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Figure 2: Effect of orally post-Administered TJ-10 on serum AST (A), ALT (B), and γ-GTP (C) activities and total blirubin concentration (D) in rats treated with and without ANIT. TJ-10 (0.26, 1.3 or 2.6 g/kg BW) was orally administered to rats treated with and without ANIT (75 mg/kg BW, i.p.) 24 h after the treatment. Rats not given TJ-10 received vehicle at the same time point. AST, ALT, γ-GTP, and total bilirubin in the serum of each rat were assayed 24 or 48 h after ANIT treatment as described in Materials and Methods. Each value is a mean ± S.D. (n = 5 for ANIT-untreated rats with and without TJ-10 administration; n = 8 for ANIT-treated rats with and without TJ-10 administration). *Significantly different from control rats without any treatment, p<0.05. #Significantly different from ANIT-treated rats without TJ-10 administration, p<0.05.

Mentions: Serum ALT and AST activities, indices of hepatic cell damage, in the ANIT-treated group significantly increased 24 h after treatment with further their increases at 48 h when compared with those in the control group (Fig. 2). TJ-10 administered orally to ANIT-treated rats at 24 h after the treatment significantly reduced the enhanced increases in serum ALT and AST activities at 48 h after the treatment at its dose of 1.3 or 2.6 g/kg BW, but not 0.26 g/kg BW (Fig. 2A and B). In the ANIT-treated group, serum γ-GTP activity and total bilirubin concentration, which are indices of biliary cell damage and cholestasis, significantly increased 24 h after treatment with further their increases at 48 h when compared with those in the control group (Fig. 2 C and D). The TJ-10 post-administered to ANIT-treated rats significantly reduced the enhanced increases in serum γ-GTP activity and bilirubin concentration at 48 h after the treatment at its dose of 1.3 or 2.6 g/kg BW, but not 0.26 g/kg BW (Fig. 2C and D). The increased serum ALT and AST activities in the ANIT-treated group given TJ-10 (2.6 g/kg BW) were near those in the ANIT-treated group found at 24 h after the treatment (Fig. 2A and B). The increased serum γ-GTP activity in the ANIT-treated group given TJ-10 (1.3 or 2.6 g/kg BW) was almost equal to that in the ANIT-treated group found at 24 h after treatment (Fig. 2C). The same doses of TJ-10 given to ANIT-untreated did not affect the serum ALT, AST, and γ-GTP activities and total bilirubin concentration (Fig. 2).


Saikokeishito Extract Exerts a Therapeutic Effect on alpha-Naphthylisothiocyanate-Induced Liver Injury in Rats through Attenuation of Enhanced Neutrophil Infiltration and Oxidative Stress in the Liver Tissue.

Ohta Y, Kongo-Nishimura M, Hayashi T, Kitagawa A, Matsura T, Yamada K - J Clin Biochem Nutr (2007)

Effect of orally post-Administered TJ-10 on serum AST (A), ALT (B), and γ-GTP (C) activities and total blirubin concentration (D) in rats treated with and without ANIT. TJ-10 (0.26, 1.3 or 2.6 g/kg BW) was orally administered to rats treated with and without ANIT (75 mg/kg BW, i.p.) 24 h after the treatment. Rats not given TJ-10 received vehicle at the same time point. AST, ALT, γ-GTP, and total bilirubin in the serum of each rat were assayed 24 or 48 h after ANIT treatment as described in Materials and Methods. Each value is a mean ± S.D. (n = 5 for ANIT-untreated rats with and without TJ-10 administration; n = 8 for ANIT-treated rats with and without TJ-10 administration). *Significantly different from control rats without any treatment, p<0.05. #Significantly different from ANIT-treated rats without TJ-10 administration, p<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 2: Effect of orally post-Administered TJ-10 on serum AST (A), ALT (B), and γ-GTP (C) activities and total blirubin concentration (D) in rats treated with and without ANIT. TJ-10 (0.26, 1.3 or 2.6 g/kg BW) was orally administered to rats treated with and without ANIT (75 mg/kg BW, i.p.) 24 h after the treatment. Rats not given TJ-10 received vehicle at the same time point. AST, ALT, γ-GTP, and total bilirubin in the serum of each rat were assayed 24 or 48 h after ANIT treatment as described in Materials and Methods. Each value is a mean ± S.D. (n = 5 for ANIT-untreated rats with and without TJ-10 administration; n = 8 for ANIT-treated rats with and without TJ-10 administration). *Significantly different from control rats without any treatment, p<0.05. #Significantly different from ANIT-treated rats without TJ-10 administration, p<0.05.
Mentions: Serum ALT and AST activities, indices of hepatic cell damage, in the ANIT-treated group significantly increased 24 h after treatment with further their increases at 48 h when compared with those in the control group (Fig. 2). TJ-10 administered orally to ANIT-treated rats at 24 h after the treatment significantly reduced the enhanced increases in serum ALT and AST activities at 48 h after the treatment at its dose of 1.3 or 2.6 g/kg BW, but not 0.26 g/kg BW (Fig. 2A and B). In the ANIT-treated group, serum γ-GTP activity and total bilirubin concentration, which are indices of biliary cell damage and cholestasis, significantly increased 24 h after treatment with further their increases at 48 h when compared with those in the control group (Fig. 2 C and D). The TJ-10 post-administered to ANIT-treated rats significantly reduced the enhanced increases in serum γ-GTP activity and bilirubin concentration at 48 h after the treatment at its dose of 1.3 or 2.6 g/kg BW, but not 0.26 g/kg BW (Fig. 2C and D). The increased serum ALT and AST activities in the ANIT-treated group given TJ-10 (2.6 g/kg BW) were near those in the ANIT-treated group found at 24 h after the treatment (Fig. 2A and B). The increased serum γ-GTP activity in the ANIT-treated group given TJ-10 (1.3 or 2.6 g/kg BW) was almost equal to that in the ANIT-treated group found at 24 h after treatment (Fig. 2C). The same doses of TJ-10 given to ANIT-untreated did not affect the serum ALT, AST, and γ-GTP activities and total bilirubin concentration (Fig. 2).

Bottom Line: At 24 h after ANIT treatment, increases in hepatic lipid peroxide and reduced glutathione contents and myeloperoxidase activity occurred with decreases in hepatic superoxide dismutase and glutathione reductase activities.At 48 h after ANIT treatment, these changes except for reduced glutathione were enhanced with decreases in catalase, Se-glutathione peroxidase, and glucose-6-phosphate dehydrogenase activities.These results indicate that TJ-10 exerts a therapeutic effect on ANIT-induced liver injury in rats possibly through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan.

ABSTRACT
We examined whether Saikokeishito extract (TJ-10), a traditional Japanese herbal medicine, exerts a therapeutic effect on alpha-naphthylisothiocyanate (ANIT)-induced liver injury in rats through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue. In rats treated once with ANIT (75 mg/kg, i.p.), liver injury with cholestasis occurred 24 h after treatment and progressed at 48 h. When ANIT-treated rats orally received TJ-10 (0.26, 1.3 or 2.6 g/kg) at 24 h after the treatment, progressive liver injury with cholestasis was significantly attenuated at 48 h after the treatment at the dose of 1.3 or 2.6 g/kg. At 24 h after ANIT treatment, increases in hepatic lipid peroxide and reduced glutathione contents and myeloperoxidase activity occurred with decreases in hepatic superoxide dismutase and glutathione reductase activities. At 48 h after ANIT treatment, these changes except for reduced glutathione were enhanced with decreases in catalase, Se-glutathione peroxidase, and glucose-6-phosphate dehydrogenase activities. TJ-10 (1.3 or 2.6 g/kg) post-administered to ANIT-treated rats attenuated these changes found at 48 h after the treatment significantly. These results indicate that TJ-10 exerts a therapeutic effect on ANIT-induced liver injury in rats possibly through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue.

No MeSH data available.


Related in: MedlinePlus