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Measurement of IgG levels can serve as a biomarker in newly diagnosed diabetic children.

Haroun M, M El-Sayed M - J Clin Biochem Nutr (2007)

Bottom Line: It was found that normal children had a mean IgG level of 7.41 mg/ml while diabetic individuals had a mean IgG level of 8.52 mg/ml (p<0.00004).Therefore, there was no significant difference in IgG level in patients with type 1 diabetes mellitus after removal of anti-cow immunoglobulin antibodies compared to normal children (p<0.58).Visualization of IgG and immuno-precipitation confirm that anti-cow immunoglobulins antibodies, which were unrelated to antigen, were co-precipitated with the antigen-antibody complex.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Technology, Institute of Graduate Studies & Research, Alexandria University, Alexandria, Egypt.

ABSTRACT
This study was undertaken to determine humoral immune response to the presence of anti-immunoglobulin antibodies in children with newly diagnosed type 1 diabetes mellitus, using as a target cow immunoglobulins, in an attempt to elucidate further complex immuno-pathogenetic interactions of the disease. Serum immunoglobulin G (IgG) concentrations were measured by ELISA in 30 children with type 1 diabetes mellitus and 30 healthy matched normal children. It was found that normal children had a mean IgG level of 7.41 mg/ml while diabetic individuals had a mean IgG level of 8.52 mg/ml (p<0.00004). On the contrary, the mean level of IgG in diabetic sera after purification from anti-cow immunoglobulins was determined to be 7.52 mg/ml. Therefore, there was no significant difference in IgG level in patients with type 1 diabetes mellitus after removal of anti-cow immunoglobulin antibodies compared to normal children (p<0.58). Visualization of IgG and immuno-precipitation confirm that anti-cow immunoglobulins antibodies, which were unrelated to antigen, were co-precipitated with the antigen-antibody complex. A circulating immunoglobulin reacting with other immunoglobulins is thus present in children with type 1 diabetes and may well play a part in the complex immuno-pathogenetic interactions.

No MeSH data available.


Related in: MedlinePlus

Visualization of IgG by immunoblotting after denaturing polyacrylamide gel electrophoresis. IgG heavy chain was detected by immunoblotting. Lanes 1 & 2 contained purified diabetic serum samples and lanes 3, 4, 5, 6 & 7 contained non-purified diabetic serum samples.
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Figure 2: Visualization of IgG by immunoblotting after denaturing polyacrylamide gel electrophoresis. IgG heavy chain was detected by immunoblotting. Lanes 1 & 2 contained purified diabetic serum samples and lanes 3, 4, 5, 6 & 7 contained non-purified diabetic serum samples.

Mentions: It is not possible to differentiate between IgG and other immunoglobulin heavy chains using polyacrylamide gel electrophoresis therefore, immunoblotting technique with anti-human IgG was carried out to determine if this increased interaction included IgG. Visual examination of Fig. 2 shows that the band intensities in diabetic sera purified from anti-cow immunoglobulin antibodies was lower than that seen in diabetic sera without pre-treatment. These results were validated by measuring the concentration of serum IgG in 5 selected diabetic and normal sera using a turbidimetric assay (Table 1).


Measurement of IgG levels can serve as a biomarker in newly diagnosed diabetic children.

Haroun M, M El-Sayed M - J Clin Biochem Nutr (2007)

Visualization of IgG by immunoblotting after denaturing polyacrylamide gel electrophoresis. IgG heavy chain was detected by immunoblotting. Lanes 1 & 2 contained purified diabetic serum samples and lanes 3, 4, 5, 6 & 7 contained non-purified diabetic serum samples.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2291473&req=5

Figure 2: Visualization of IgG by immunoblotting after denaturing polyacrylamide gel electrophoresis. IgG heavy chain was detected by immunoblotting. Lanes 1 & 2 contained purified diabetic serum samples and lanes 3, 4, 5, 6 & 7 contained non-purified diabetic serum samples.
Mentions: It is not possible to differentiate between IgG and other immunoglobulin heavy chains using polyacrylamide gel electrophoresis therefore, immunoblotting technique with anti-human IgG was carried out to determine if this increased interaction included IgG. Visual examination of Fig. 2 shows that the band intensities in diabetic sera purified from anti-cow immunoglobulin antibodies was lower than that seen in diabetic sera without pre-treatment. These results were validated by measuring the concentration of serum IgG in 5 selected diabetic and normal sera using a turbidimetric assay (Table 1).

Bottom Line: It was found that normal children had a mean IgG level of 7.41 mg/ml while diabetic individuals had a mean IgG level of 8.52 mg/ml (p<0.00004).Therefore, there was no significant difference in IgG level in patients with type 1 diabetes mellitus after removal of anti-cow immunoglobulin antibodies compared to normal children (p<0.58).Visualization of IgG and immuno-precipitation confirm that anti-cow immunoglobulins antibodies, which were unrelated to antigen, were co-precipitated with the antigen-antibody complex.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Technology, Institute of Graduate Studies & Research, Alexandria University, Alexandria, Egypt.

ABSTRACT
This study was undertaken to determine humoral immune response to the presence of anti-immunoglobulin antibodies in children with newly diagnosed type 1 diabetes mellitus, using as a target cow immunoglobulins, in an attempt to elucidate further complex immuno-pathogenetic interactions of the disease. Serum immunoglobulin G (IgG) concentrations were measured by ELISA in 30 children with type 1 diabetes mellitus and 30 healthy matched normal children. It was found that normal children had a mean IgG level of 7.41 mg/ml while diabetic individuals had a mean IgG level of 8.52 mg/ml (p<0.00004). On the contrary, the mean level of IgG in diabetic sera after purification from anti-cow immunoglobulins was determined to be 7.52 mg/ml. Therefore, there was no significant difference in IgG level in patients with type 1 diabetes mellitus after removal of anti-cow immunoglobulin antibodies compared to normal children (p<0.58). Visualization of IgG and immuno-precipitation confirm that anti-cow immunoglobulins antibodies, which were unrelated to antigen, were co-precipitated with the antigen-antibody complex. A circulating immunoglobulin reacting with other immunoglobulins is thus present in children with type 1 diabetes and may well play a part in the complex immuno-pathogenetic interactions.

No MeSH data available.


Related in: MedlinePlus