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Up-regulation of multiple proteins and biological processes during maxillary expansion in rats.

Ma J, Wu Y, Zhang W, Smales RJ, Huang Y, Pan Y, Wang L - BMC Musculoskelet Disord (2008)

Bottom Line: In order to develop a more effective method, this study was designed to further examine the process of tissue remodeling during ME, to identify the changes in expression of several proteins of interest, and to clarify the molecular mechanism responsible for tissue remodeling.The changes in three proteins closely related to osteogenesis (parathyroid hormone, osteoprotegerin and vimentin) were confirmed by Western blotting.Many proteins are over-expressed during ME, and they may play an important role in the remodeling process.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Stomatology, School of Stomatology, Nanjing Medical University, PR China. majunq@163.com

ABSTRACT

Background: Maxillary expansion (ME) is a common practice in orthodontics that aims to increase the constricted maxillary arch width. Relapse often occurs, however, and better treatment strategies are needed. In order to develop a more effective method, this study was designed to further examine the process of tissue remodeling during ME, to identify the changes in expression of several proteins of interest, and to clarify the molecular mechanism responsible for tissue remodeling.

Methods: Male Wistar rats were randomly divided into control and ME groups. The rats were euthanized at various intervals over 11 days, and the dissected palates were prepared for histological examination. The structure of the midpalatal sutures changed little during the first three days. Proteins from samples in the ground midpalatal tissues obtained on the third day were subjected to two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. Validation of protein expression was performed by Western blot analyses.

Results: From day 5, chondrocytes in the inner layer of suture cartilage and osteoblasts at the end of the suture cartilage began to proliferate, and the skeletal matrix increased later adjacent to the cartilage in the ME group. Comparative proteomic analysis showed increases in 22 protein spots present in the ME group. The changes in three proteins closely related to osteogenesis (parathyroid hormone, osteoprotegerin and vimentin) were confirmed by Western blotting.

Conclusion: Many proteins are over-expressed during ME, and they may play an important role in the remodeling process.

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Related in: MedlinePlus

PCNA expression in the midpalatal suture. SC: suture cartilage. SB: suture bone. On day 5, some of the chondrocytes in the proliferating layer of the suture cartilage and osteoblasts at the end of the cartilage were PCNA-positive in the ME group. On day 11, many osteoblasts adjacent to the bone trabeculae were positive in the ME group. (Original magnification ×40).
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Figure 4: PCNA expression in the midpalatal suture. SC: suture cartilage. SB: suture bone. On day 5, some of the chondrocytes in the proliferating layer of the suture cartilage and osteoblasts at the end of the cartilage were PCNA-positive in the ME group. On day 11, many osteoblasts adjacent to the bone trabeculae were positive in the ME group. (Original magnification ×40).

Mentions: During the first three days, few PCNA-positive cells were observed in either group. From day 5, some of the chondrocytes in the inner layer of suture cartilage and osteoblasts at the end of the suture cartilage were PCNA positive in the ME group (Figure 4). On day 11, many osteoblasts adjacent to bone trabeculae were positive in the ME group (Figure 4). To identify the tissue (marked with "T") in the suture shown in Figure 2H of the ME group, we performed immunohistochemical analysis of Collagen I and found that it was bone tissue, but not residual suture cartilage (Figure 5).


Up-regulation of multiple proteins and biological processes during maxillary expansion in rats.

Ma J, Wu Y, Zhang W, Smales RJ, Huang Y, Pan Y, Wang L - BMC Musculoskelet Disord (2008)

PCNA expression in the midpalatal suture. SC: suture cartilage. SB: suture bone. On day 5, some of the chondrocytes in the proliferating layer of the suture cartilage and osteoblasts at the end of the cartilage were PCNA-positive in the ME group. On day 11, many osteoblasts adjacent to the bone trabeculae were positive in the ME group. (Original magnification ×40).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2291465&req=5

Figure 4: PCNA expression in the midpalatal suture. SC: suture cartilage. SB: suture bone. On day 5, some of the chondrocytes in the proliferating layer of the suture cartilage and osteoblasts at the end of the cartilage were PCNA-positive in the ME group. On day 11, many osteoblasts adjacent to the bone trabeculae were positive in the ME group. (Original magnification ×40).
Mentions: During the first three days, few PCNA-positive cells were observed in either group. From day 5, some of the chondrocytes in the inner layer of suture cartilage and osteoblasts at the end of the suture cartilage were PCNA positive in the ME group (Figure 4). On day 11, many osteoblasts adjacent to bone trabeculae were positive in the ME group (Figure 4). To identify the tissue (marked with "T") in the suture shown in Figure 2H of the ME group, we performed immunohistochemical analysis of Collagen I and found that it was bone tissue, but not residual suture cartilage (Figure 5).

Bottom Line: In order to develop a more effective method, this study was designed to further examine the process of tissue remodeling during ME, to identify the changes in expression of several proteins of interest, and to clarify the molecular mechanism responsible for tissue remodeling.The changes in three proteins closely related to osteogenesis (parathyroid hormone, osteoprotegerin and vimentin) were confirmed by Western blotting.Many proteins are over-expressed during ME, and they may play an important role in the remodeling process.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Stomatology, School of Stomatology, Nanjing Medical University, PR China. majunq@163.com

ABSTRACT

Background: Maxillary expansion (ME) is a common practice in orthodontics that aims to increase the constricted maxillary arch width. Relapse often occurs, however, and better treatment strategies are needed. In order to develop a more effective method, this study was designed to further examine the process of tissue remodeling during ME, to identify the changes in expression of several proteins of interest, and to clarify the molecular mechanism responsible for tissue remodeling.

Methods: Male Wistar rats were randomly divided into control and ME groups. The rats were euthanized at various intervals over 11 days, and the dissected palates were prepared for histological examination. The structure of the midpalatal sutures changed little during the first three days. Proteins from samples in the ground midpalatal tissues obtained on the third day were subjected to two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. Validation of protein expression was performed by Western blot analyses.

Results: From day 5, chondrocytes in the inner layer of suture cartilage and osteoblasts at the end of the suture cartilage began to proliferate, and the skeletal matrix increased later adjacent to the cartilage in the ME group. Comparative proteomic analysis showed increases in 22 protein spots present in the ME group. The changes in three proteins closely related to osteogenesis (parathyroid hormone, osteoprotegerin and vimentin) were confirmed by Western blotting.

Conclusion: Many proteins are over-expressed during ME, and they may play an important role in the remodeling process.

Show MeSH
Related in: MedlinePlus