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AtMRP6/AtABCC6, an ATP-binding cassette transporter gene expressed during early steps of seedling development and up-regulated by cadmium in Arabidopsis thaliana.

Gaillard S, Jacquet H, Vavasseur A, Leonhardt N, Forestier C - BMC Plant Biol. (2008)

Bottom Line: ABC proteins constitute one of the largest families of transporters found in all living organisms.Using real-time quantitative PCR (RT-Q-PCR) and the GUS reporter gene, we found that this gene is essentially expressed during early seedling development, in the apical meristem and at initiation point of secondary roots, especially in xylem-opposite pericycle cells where lateral roots initiate.The position of AtMRP6 on chromosome 3, flanked by two other MRP genes, (all of which being induced by Cd) suggests that AtMRP6 is part of a cluster involved in metal tolerance, although additional functions in planta cannot be discarded.

View Article: PubMed Central - HTML - PubMed

Affiliation: CEA, DSV, IBEB, Lab Echanges Membranaires & Signalisation, Saint-Paul-lez-Durance, F-13108, France. sgaillard@ibdml.univ-mrs.fr

ABSTRACT

Background: ABC proteins constitute one of the largest families of transporters found in all living organisms. In Arabidopsis thaliana, 120 genes encoding ABC transporters have been identified. Here, the characterization of one member of the MRP subclass, AtMRP6, is described.

Results: This gene, located on chromosome 3, is bordered by AtMRP3 and AtMRP7. Using real-time quantitative PCR (RT-Q-PCR) and the GUS reporter gene, we found that this gene is essentially expressed during early seedling development, in the apical meristem and at initiation point of secondary roots, especially in xylem-opposite pericycle cells where lateral roots initiate. The level of expression of AtMRP6 in response to various stresses was explored and a significant up-regulation after cadmium (Cd) treatment was detected. Among the three T-DNA insertion lines available from the Salk Institute library, two knock-out mutants, Atmrp6.1 and Atmrp6.2 were invalidated for the AtMRP6 gene. In the presence of Cd, development of leaves was more affected in the mutants than wild-type plants, whereas root elongation and ramification was comparable.

Conclusion: The position of AtMRP6 on chromosome 3, flanked by two other MRP genes, (all of which being induced by Cd) suggests that AtMRP6 is part of a cluster involved in metal tolerance, although additional functions in planta cannot be discarded.

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Modulation of AtMRP6 gene expression level determined by quantitative real-time PCR in response to different stress conditions. Variation of AtMRP6 gene expression in seedlings treated with different hormones (100 μM, 12-hr), after an oxidative stress (10 mM H2O2, 12-hr) or in roots of 3–4 week-old plants after Cd exposure (5 μM, 30-hr). (ABA: abscissic acid, GA: gibberillic acid, MJ: methyl jasmonate). Values from three independent experiments are expressed as percentage of control (untreated plants). (* : P < 0.05, t-test).
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Figure 4: Modulation of AtMRP6 gene expression level determined by quantitative real-time PCR in response to different stress conditions. Variation of AtMRP6 gene expression in seedlings treated with different hormones (100 μM, 12-hr), after an oxidative stress (10 mM H2O2, 12-hr) or in roots of 3–4 week-old plants after Cd exposure (5 μM, 30-hr). (ABA: abscissic acid, GA: gibberillic acid, MJ: methyl jasmonate). Values from three independent experiments are expressed as percentage of control (untreated plants). (* : P < 0.05, t-test).

Mentions: In order to determine in which process AtMRP6 could be involved, its expression level in response to numerous stresses was investigated by RT-Q-PCR in Arabidopsis plantlets. A significant variation of AtMRP6 expression level was observed after hydrogen peroxide treatment but not in response to hormones (brassinosteroid, abscisic acid and analogous-compounds, gibberillic acid or methyl jasmonate, figure 4) or to salt or cold stress (data not shown). Concomitantly by a transcriptomic analysis of genes regulated by Cd [39], we observed that AtMRP6 was one of the most induced ABC transporter genes. Such an up-regulation by Cd was confirmed by RT-Q-PCR, AtMRP6 being up-regulated in roots after a 30-hr exposition to 5 μM Cd (figure 4).


AtMRP6/AtABCC6, an ATP-binding cassette transporter gene expressed during early steps of seedling development and up-regulated by cadmium in Arabidopsis thaliana.

Gaillard S, Jacquet H, Vavasseur A, Leonhardt N, Forestier C - BMC Plant Biol. (2008)

Modulation of AtMRP6 gene expression level determined by quantitative real-time PCR in response to different stress conditions. Variation of AtMRP6 gene expression in seedlings treated with different hormones (100 μM, 12-hr), after an oxidative stress (10 mM H2O2, 12-hr) or in roots of 3–4 week-old plants after Cd exposure (5 μM, 30-hr). (ABA: abscissic acid, GA: gibberillic acid, MJ: methyl jasmonate). Values from three independent experiments are expressed as percentage of control (untreated plants). (* : P < 0.05, t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2291051&req=5

Figure 4: Modulation of AtMRP6 gene expression level determined by quantitative real-time PCR in response to different stress conditions. Variation of AtMRP6 gene expression in seedlings treated with different hormones (100 μM, 12-hr), after an oxidative stress (10 mM H2O2, 12-hr) or in roots of 3–4 week-old plants after Cd exposure (5 μM, 30-hr). (ABA: abscissic acid, GA: gibberillic acid, MJ: methyl jasmonate). Values from three independent experiments are expressed as percentage of control (untreated plants). (* : P < 0.05, t-test).
Mentions: In order to determine in which process AtMRP6 could be involved, its expression level in response to numerous stresses was investigated by RT-Q-PCR in Arabidopsis plantlets. A significant variation of AtMRP6 expression level was observed after hydrogen peroxide treatment but not in response to hormones (brassinosteroid, abscisic acid and analogous-compounds, gibberillic acid or methyl jasmonate, figure 4) or to salt or cold stress (data not shown). Concomitantly by a transcriptomic analysis of genes regulated by Cd [39], we observed that AtMRP6 was one of the most induced ABC transporter genes. Such an up-regulation by Cd was confirmed by RT-Q-PCR, AtMRP6 being up-regulated in roots after a 30-hr exposition to 5 μM Cd (figure 4).

Bottom Line: ABC proteins constitute one of the largest families of transporters found in all living organisms.Using real-time quantitative PCR (RT-Q-PCR) and the GUS reporter gene, we found that this gene is essentially expressed during early seedling development, in the apical meristem and at initiation point of secondary roots, especially in xylem-opposite pericycle cells where lateral roots initiate.The position of AtMRP6 on chromosome 3, flanked by two other MRP genes, (all of which being induced by Cd) suggests that AtMRP6 is part of a cluster involved in metal tolerance, although additional functions in planta cannot be discarded.

View Article: PubMed Central - HTML - PubMed

Affiliation: CEA, DSV, IBEB, Lab Echanges Membranaires & Signalisation, Saint-Paul-lez-Durance, F-13108, France. sgaillard@ibdml.univ-mrs.fr

ABSTRACT

Background: ABC proteins constitute one of the largest families of transporters found in all living organisms. In Arabidopsis thaliana, 120 genes encoding ABC transporters have been identified. Here, the characterization of one member of the MRP subclass, AtMRP6, is described.

Results: This gene, located on chromosome 3, is bordered by AtMRP3 and AtMRP7. Using real-time quantitative PCR (RT-Q-PCR) and the GUS reporter gene, we found that this gene is essentially expressed during early seedling development, in the apical meristem and at initiation point of secondary roots, especially in xylem-opposite pericycle cells where lateral roots initiate. The level of expression of AtMRP6 in response to various stresses was explored and a significant up-regulation after cadmium (Cd) treatment was detected. Among the three T-DNA insertion lines available from the Salk Institute library, two knock-out mutants, Atmrp6.1 and Atmrp6.2 were invalidated for the AtMRP6 gene. In the presence of Cd, development of leaves was more affected in the mutants than wild-type plants, whereas root elongation and ramification was comparable.

Conclusion: The position of AtMRP6 on chromosome 3, flanked by two other MRP genes, (all of which being induced by Cd) suggests that AtMRP6 is part of a cluster involved in metal tolerance, although additional functions in planta cannot be discarded.

Show MeSH
Related in: MedlinePlus