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Competition of Lactobacillus paracasei with Salmonella enterica for adhesion to Caco-2 cells.

Jankowska A, Laubitz D, Antushevich H, Zabielski R, Grzesiuk E - J. Biomed. Biotechnol. (2008)

Bottom Line: Caco-2 cells were grown for 6 or 21 days to obtain nondifferentiated or well-differentiated cells, respectively.Both bacterial strains used adhered better to well-differentiated than to nondifferentiated Caco-2 cells, however, the amount of Salmonella adhered to Caco-2 after 2 hours of contact was 12-fold higher in comparison to L. paracasei and almost 27-fold higher after 4 hours of contact.In conclusion, the displacement of pathogens by lactic acid bacteria and its secretions showed here depends on the time of bacteria-epithelial cell contact, and also on the stage of Caco-2 differentiation.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Molecular Biology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland.

ABSTRACT
Competition of commensal and probiotic bacteria with pathogens for adhesion and colonization is one of the important protective mechanisms of gastrointestinal tract. In this study, we examined the ability of Lactobacillus paracasei to inhibit the adhesion of pathogenic Salmonella enterica to human colon adenocarcinoma Caco-2 cells. Caco-2 cells were grown for 6 or 21 days to obtain nondifferentiated or well-differentiated cells, respectively. In adhesion experiments, bacteria were added to the cells for 2 or 4 hours. The number of attached bacteria was expressed as colony-forming units (CFUs), Caco-2 cells were counted in hematocytometer. Both bacterial strains used adhered better to well-differentiated than to nondifferentiated Caco-2 cells, however, the amount of Salmonella adhered to Caco-2 after 2 hours of contact was 12-fold higher in comparison to L. paracasei and almost 27-fold higher after 4 hours of contact. Two types of experiments were done: coincubation (both bacteria were added to Caco-2 cells simultaneously), and preincubation (L. paracasei was incubated with Caco-2 cells first, and then S. enterica was added). In coincubation experiment, the presence of L. paracasei decreased S. enterica adhesion by 4-fold and in preincubation experiment even 7-fold. Generally, Lactobacillus spent culture supernatants (SCSs) acted weaker as inhibitors of Salmonella adhesion in comparison to the whole L. paracasei culture in coincubation experiment. In conclusion, the displacement of pathogens by lactic acid bacteria and its secretions showed here depends on the time of bacteria-epithelial cell contact, and also on the stage of Caco-2 differentiation.

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Adhesion of S. enterica to Caco-2 cells preincubated with L. paracasei or its culture spent supernatant (SCS) as indicated. Bars represent mean values with respective SEM. Asterisks indicate the statistical differences: *P < .05, **P < .01, ***P < .001 (one-way ANOVA followed by Tukey posthoc test).
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fig4: Adhesion of S. enterica to Caco-2 cells preincubated with L. paracasei or its culture spent supernatant (SCS) as indicated. Bars represent mean values with respective SEM. Asterisks indicate the statistical differences: *P < .05, **P < .01, ***P < .001 (one-way ANOVA followed by Tukey posthoc test).

Mentions: Preincubation of L. paracasei with Caco-2 cells for 2 hours and subsequent addition of S. enterica resulted in over 2-fold stronger inhibition of Salmonella adherence than in coincubation experiment. This means that Lactobacillus inhibited Salmonella adherence 8- and 4-fold in pre- and coincubation experiment, respectively (Figures2 and 4). The effect of preincubation was, however, statistically significant only in the 2-hour (P < .05) but not in the 4-hour coincubation experiment (Figure 4).


Competition of Lactobacillus paracasei with Salmonella enterica for adhesion to Caco-2 cells.

Jankowska A, Laubitz D, Antushevich H, Zabielski R, Grzesiuk E - J. Biomed. Biotechnol. (2008)

Adhesion of S. enterica to Caco-2 cells preincubated with L. paracasei or its culture spent supernatant (SCS) as indicated. Bars represent mean values with respective SEM. Asterisks indicate the statistical differences: *P < .05, **P < .01, ***P < .001 (one-way ANOVA followed by Tukey posthoc test).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2279207&req=5

fig4: Adhesion of S. enterica to Caco-2 cells preincubated with L. paracasei or its culture spent supernatant (SCS) as indicated. Bars represent mean values with respective SEM. Asterisks indicate the statistical differences: *P < .05, **P < .01, ***P < .001 (one-way ANOVA followed by Tukey posthoc test).
Mentions: Preincubation of L. paracasei with Caco-2 cells for 2 hours and subsequent addition of S. enterica resulted in over 2-fold stronger inhibition of Salmonella adherence than in coincubation experiment. This means that Lactobacillus inhibited Salmonella adherence 8- and 4-fold in pre- and coincubation experiment, respectively (Figures2 and 4). The effect of preincubation was, however, statistically significant only in the 2-hour (P < .05) but not in the 4-hour coincubation experiment (Figure 4).

Bottom Line: Caco-2 cells were grown for 6 or 21 days to obtain nondifferentiated or well-differentiated cells, respectively.Both bacterial strains used adhered better to well-differentiated than to nondifferentiated Caco-2 cells, however, the amount of Salmonella adhered to Caco-2 after 2 hours of contact was 12-fold higher in comparison to L. paracasei and almost 27-fold higher after 4 hours of contact.In conclusion, the displacement of pathogens by lactic acid bacteria and its secretions showed here depends on the time of bacteria-epithelial cell contact, and also on the stage of Caco-2 differentiation.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Molecular Biology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland.

ABSTRACT
Competition of commensal and probiotic bacteria with pathogens for adhesion and colonization is one of the important protective mechanisms of gastrointestinal tract. In this study, we examined the ability of Lactobacillus paracasei to inhibit the adhesion of pathogenic Salmonella enterica to human colon adenocarcinoma Caco-2 cells. Caco-2 cells were grown for 6 or 21 days to obtain nondifferentiated or well-differentiated cells, respectively. In adhesion experiments, bacteria were added to the cells for 2 or 4 hours. The number of attached bacteria was expressed as colony-forming units (CFUs), Caco-2 cells were counted in hematocytometer. Both bacterial strains used adhered better to well-differentiated than to nondifferentiated Caco-2 cells, however, the amount of Salmonella adhered to Caco-2 after 2 hours of contact was 12-fold higher in comparison to L. paracasei and almost 27-fold higher after 4 hours of contact. Two types of experiments were done: coincubation (both bacteria were added to Caco-2 cells simultaneously), and preincubation (L. paracasei was incubated with Caco-2 cells first, and then S. enterica was added). In coincubation experiment, the presence of L. paracasei decreased S. enterica adhesion by 4-fold and in preincubation experiment even 7-fold. Generally, Lactobacillus spent culture supernatants (SCSs) acted weaker as inhibitors of Salmonella adhesion in comparison to the whole L. paracasei culture in coincubation experiment. In conclusion, the displacement of pathogens by lactic acid bacteria and its secretions showed here depends on the time of bacteria-epithelial cell contact, and also on the stage of Caco-2 differentiation.

Show MeSH
Related in: MedlinePlus