Limits...
A micro-fluidic study of whole blood behaviour on PMMA topographical nanostructures.

Minelli C, Kikuta A, Tsud N, Ball MD, Yamamoto A - J Nanobiotechnology (2008)

Bottom Line: Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm.Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size.

View Article: PubMed Central - HTML - PubMed

Affiliation: International Centre for Young Scientists, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. c.minelli@imperial.ac.uk.

ABSTRACT

Background: Polymers are attractive materials for both biomedical engineering and cardiovascular applications. Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.

Results: We optimized a micro-fluidic set-up to study the interaction of whole blood with nano-structured polymer surfaces under flow conditions. Micro-fluidic chips were coated with polymethylmethacrylate films and structured by polymer demixing. Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm. Whole blood flow rate through the micro-fluidic channels, platelet adhesion and von Willebrand factor and fibrinogen adsorption onto the structured polymer films were investigated. Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size. Adhesion and spreading of platelets from whole blood and von Willebrand factor adsorption from platelet poor plasma were enhanced on the structured surfaces with larger feature, while fibrinogen adsorption followed the opposite trend.

Conclusion: We investigated whole blood behaviour and plasma protein adsorption on nano-structured polymer materials under flow conditions using a micro-fluidic set-up. We speculate that surface nano-topography of polymer films influences primarily plasma protein adsorption, which results in the control of platelet adhesion and thrombus formation.

No MeSH data available.


Related in: MedlinePlus

Plasma protein adsorption analysis. (A) SEM image of gold and silver labelled von Willebrand factors proteins on a PMMA3 surface. (B) Statistical protein distribution on chips having different surface topographies as reported in Table 1. Each bar represents the average silver surface coverage evaluated over 20 SEM images having the same surface area. The data are normalized to the bare chip surface.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2279145&req=5

Figure 6: Plasma protein adsorption analysis. (A) SEM image of gold and silver labelled von Willebrand factors proteins on a PMMA3 surface. (B) Statistical protein distribution on chips having different surface topographies as reported in Table 1. Each bar represents the average silver surface coverage evaluated over 20 SEM images having the same surface area. The data are normalized to the bare chip surface.

Mentions: Figure 6A shows a typical SEM image of gold and silver labelled von Willebrand factor adsorbed from platelet poor plasma onto a PMMA structured surface. Figure 6B shows the analysis of fibrinogen and von Willebrand factor distribution on SiO2 (reference material), PMMA1, PMMA2, PMMA3 and PMMA4 surfaces. Each histogram bar represents the average of the protein coverage distribution calculated from 20 SEM images having the same area as Figure 6A, while the error bar is the standard deviation of the distribution. The results are normalized to the protein adsorption onto the SiO2 surface of a bare chip. Fibrinogen adsorption onto surfaces PMMA1, PMMA2 and PMMA3 is comparable, while it is significantly reduced on PMMA4. Von Willebrand factor adsorption is favoured on structured PMMA surfaces with respect to flat surfaces, and increased on surfaces with larger feature sizes.


A micro-fluidic study of whole blood behaviour on PMMA topographical nanostructures.

Minelli C, Kikuta A, Tsud N, Ball MD, Yamamoto A - J Nanobiotechnology (2008)

Plasma protein adsorption analysis. (A) SEM image of gold and silver labelled von Willebrand factors proteins on a PMMA3 surface. (B) Statistical protein distribution on chips having different surface topographies as reported in Table 1. Each bar represents the average silver surface coverage evaluated over 20 SEM images having the same surface area. The data are normalized to the bare chip surface.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2279145&req=5

Figure 6: Plasma protein adsorption analysis. (A) SEM image of gold and silver labelled von Willebrand factors proteins on a PMMA3 surface. (B) Statistical protein distribution on chips having different surface topographies as reported in Table 1. Each bar represents the average silver surface coverage evaluated over 20 SEM images having the same surface area. The data are normalized to the bare chip surface.
Mentions: Figure 6A shows a typical SEM image of gold and silver labelled von Willebrand factor adsorbed from platelet poor plasma onto a PMMA structured surface. Figure 6B shows the analysis of fibrinogen and von Willebrand factor distribution on SiO2 (reference material), PMMA1, PMMA2, PMMA3 and PMMA4 surfaces. Each histogram bar represents the average of the protein coverage distribution calculated from 20 SEM images having the same area as Figure 6A, while the error bar is the standard deviation of the distribution. The results are normalized to the protein adsorption onto the SiO2 surface of a bare chip. Fibrinogen adsorption onto surfaces PMMA1, PMMA2 and PMMA3 is comparable, while it is significantly reduced on PMMA4. Von Willebrand factor adsorption is favoured on structured PMMA surfaces with respect to flat surfaces, and increased on surfaces with larger feature sizes.

Bottom Line: Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm.Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size.

View Article: PubMed Central - HTML - PubMed

Affiliation: International Centre for Young Scientists, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. c.minelli@imperial.ac.uk.

ABSTRACT

Background: Polymers are attractive materials for both biomedical engineering and cardiovascular applications. Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.

Results: We optimized a micro-fluidic set-up to study the interaction of whole blood with nano-structured polymer surfaces under flow conditions. Micro-fluidic chips were coated with polymethylmethacrylate films and structured by polymer demixing. Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm. Whole blood flow rate through the micro-fluidic channels, platelet adhesion and von Willebrand factor and fibrinogen adsorption onto the structured polymer films were investigated. Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size. Adhesion and spreading of platelets from whole blood and von Willebrand factor adsorption from platelet poor plasma were enhanced on the structured surfaces with larger feature, while fibrinogen adsorption followed the opposite trend.

Conclusion: We investigated whole blood behaviour and plasma protein adsorption on nano-structured polymer materials under flow conditions using a micro-fluidic set-up. We speculate that surface nano-topography of polymer films influences primarily plasma protein adsorption, which results in the control of platelet adhesion and thrombus formation.

No MeSH data available.


Related in: MedlinePlus