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A micro-fluidic study of whole blood behaviour on PMMA topographical nanostructures.

Minelli C, Kikuta A, Tsud N, Ball MD, Yamamoto A - J Nanobiotechnology (2008)

Bottom Line: Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm.Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size.

View Article: PubMed Central - HTML - PubMed

Affiliation: International Centre for Young Scientists, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. c.minelli@imperial.ac.uk.

ABSTRACT

Background: Polymers are attractive materials for both biomedical engineering and cardiovascular applications. Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.

Results: We optimized a micro-fluidic set-up to study the interaction of whole blood with nano-structured polymer surfaces under flow conditions. Micro-fluidic chips were coated with polymethylmethacrylate films and structured by polymer demixing. Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm. Whole blood flow rate through the micro-fluidic channels, platelet adhesion and von Willebrand factor and fibrinogen adsorption onto the structured polymer films were investigated. Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size. Adhesion and spreading of platelets from whole blood and von Willebrand factor adsorption from platelet poor plasma were enhanced on the structured surfaces with larger feature, while fibrinogen adsorption followed the opposite trend.

Conclusion: We investigated whole blood behaviour and plasma protein adsorption on nano-structured polymer materials under flow conditions using a micro-fluidic set-up. We speculate that surface nano-topography of polymer films influences primarily plasma protein adsorption, which results in the control of platelet adhesion and thrombus formation.

No MeSH data available.


Related in: MedlinePlus

Platelet morphology. SEM images of platelets adhered onto topographically structured PMMA surfaces after the BPT measurements. (A) Large view of the micro-channels coated with PMMA3. (B) Detail of a micro-channel coated with PMMA2, (C) PMMA3, (D) PMMA4.
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Figure 4: Platelet morphology. SEM images of platelets adhered onto topographically structured PMMA surfaces after the BPT measurements. (A) Large view of the micro-channels coated with PMMA3. (B) Detail of a micro-channel coated with PMMA2, (C) PMMA3, (D) PMMA4.

Mentions: Optical investigation of the chip surface before and after rinsing showed the presence of a higher density of firmly adhered platelets on films with larger feature size. The chip surfaces were investigated using Scanning Electron Microscopy (SEM) after the micro-fluidic experiments and platelet fixation. Figure 4A shows a low magnification image of a part of the chip, where several platelets are seen to adhere along the micro-channel walls and in the areas around them. Closer views of the channel walls are shown in Figures 4B, C and 4D, for the PMMA2, PMMA3 and PMMA4 surfaces respectively. Different platelet morphologies are observed in the three cases. Platelets clearly anchor to the three polymer films. Platelets appeared rounded on PMMA2 and 3, and more flattened and interconnected on the PMMA4 surface. Platelets adhered on PMMA2 film have a smoother surface with respect to those on PMMA3.


A micro-fluidic study of whole blood behaviour on PMMA topographical nanostructures.

Minelli C, Kikuta A, Tsud N, Ball MD, Yamamoto A - J Nanobiotechnology (2008)

Platelet morphology. SEM images of platelets adhered onto topographically structured PMMA surfaces after the BPT measurements. (A) Large view of the micro-channels coated with PMMA3. (B) Detail of a micro-channel coated with PMMA2, (C) PMMA3, (D) PMMA4.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2279145&req=5

Figure 4: Platelet morphology. SEM images of platelets adhered onto topographically structured PMMA surfaces after the BPT measurements. (A) Large view of the micro-channels coated with PMMA3. (B) Detail of a micro-channel coated with PMMA2, (C) PMMA3, (D) PMMA4.
Mentions: Optical investigation of the chip surface before and after rinsing showed the presence of a higher density of firmly adhered platelets on films with larger feature size. The chip surfaces were investigated using Scanning Electron Microscopy (SEM) after the micro-fluidic experiments and platelet fixation. Figure 4A shows a low magnification image of a part of the chip, where several platelets are seen to adhere along the micro-channel walls and in the areas around them. Closer views of the channel walls are shown in Figures 4B, C and 4D, for the PMMA2, PMMA3 and PMMA4 surfaces respectively. Different platelet morphologies are observed in the three cases. Platelets clearly anchor to the three polymer films. Platelets appeared rounded on PMMA2 and 3, and more flattened and interconnected on the PMMA4 surface. Platelets adhered on PMMA2 film have a smoother surface with respect to those on PMMA3.

Bottom Line: Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm.Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size.

View Article: PubMed Central - HTML - PubMed

Affiliation: International Centre for Young Scientists, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. c.minelli@imperial.ac.uk.

ABSTRACT

Background: Polymers are attractive materials for both biomedical engineering and cardiovascular applications. Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.

Results: We optimized a micro-fluidic set-up to study the interaction of whole blood with nano-structured polymer surfaces under flow conditions. Micro-fluidic chips were coated with polymethylmethacrylate films and structured by polymer demixing. Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm. Whole blood flow rate through the micro-fluidic channels, platelet adhesion and von Willebrand factor and fibrinogen adsorption onto the structured polymer films were investigated. Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size. Adhesion and spreading of platelets from whole blood and von Willebrand factor adsorption from platelet poor plasma were enhanced on the structured surfaces with larger feature, while fibrinogen adsorption followed the opposite trend.

Conclusion: We investigated whole blood behaviour and plasma protein adsorption on nano-structured polymer materials under flow conditions using a micro-fluidic set-up. We speculate that surface nano-topography of polymer films influences primarily plasma protein adsorption, which results in the control of platelet adhesion and thrombus formation.

No MeSH data available.


Related in: MedlinePlus