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A micro-fluidic study of whole blood behaviour on PMMA topographical nanostructures.

Minelli C, Kikuta A, Tsud N, Ball MD, Yamamoto A - J Nanobiotechnology (2008)

Bottom Line: Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm.Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size.

View Article: PubMed Central - HTML - PubMed

Affiliation: International Centre for Young Scientists, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. c.minelli@imperial.ac.uk.

ABSTRACT

Background: Polymers are attractive materials for both biomedical engineering and cardiovascular applications. Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.

Results: We optimized a micro-fluidic set-up to study the interaction of whole blood with nano-structured polymer surfaces under flow conditions. Micro-fluidic chips were coated with polymethylmethacrylate films and structured by polymer demixing. Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm. Whole blood flow rate through the micro-fluidic channels, platelet adhesion and von Willebrand factor and fibrinogen adsorption onto the structured polymer films were investigated. Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size. Adhesion and spreading of platelets from whole blood and von Willebrand factor adsorption from platelet poor plasma were enhanced on the structured surfaces with larger feature, while fibrinogen adsorption followed the opposite trend.

Conclusion: We investigated whole blood behaviour and plasma protein adsorption on nano-structured polymer materials under flow conditions using a micro-fluidic set-up. We speculate that surface nano-topography of polymer films influences primarily plasma protein adsorption, which results in the control of platelet adhesion and thrombus formation.

No MeSH data available.


Related in: MedlinePlus

The Micro-fluidic experiment set up. (A) Schematic of the MC-FUN set up (not to scale). (B) Top view of a micro-fluidic chip (15 mm × 15 mm). (C) Particulars of the micro-channels. (D) Geometrical parameters of the micro-fluidic chip.
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Figure 1: The Micro-fluidic experiment set up. (A) Schematic of the MC-FUN set up (not to scale). (B) Top view of a micro-fluidic chip (15 mm × 15 mm). (C) Particulars of the micro-channels. (D) Geometrical parameters of the micro-fluidic chip.

Mentions: The study is performed by using a Micro-Channel Array Flow Analyzer (MC-FAN, Figure 1A), which was previously utilized to characterize the interaction of whole blood and plasma proteins with metal surfaces providing interesting insights into the importance of surface energy on blood coagulation mechanism [22]. Our intention is to demonstrate that the MC-FAN is a viable in vitro set-up for the study of the interaction of blood with a large class of polymers and surfaces and thus for a first-stage selection of potential blood compatible materials, avoiding the costs, the long times and the sacrifice of animals required by in vivo experiments.


A micro-fluidic study of whole blood behaviour on PMMA topographical nanostructures.

Minelli C, Kikuta A, Tsud N, Ball MD, Yamamoto A - J Nanobiotechnology (2008)

The Micro-fluidic experiment set up. (A) Schematic of the MC-FUN set up (not to scale). (B) Top view of a micro-fluidic chip (15 mm × 15 mm). (C) Particulars of the micro-channels. (D) Geometrical parameters of the micro-fluidic chip.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2279145&req=5

Figure 1: The Micro-fluidic experiment set up. (A) Schematic of the MC-FUN set up (not to scale). (B) Top view of a micro-fluidic chip (15 mm × 15 mm). (C) Particulars of the micro-channels. (D) Geometrical parameters of the micro-fluidic chip.
Mentions: The study is performed by using a Micro-Channel Array Flow Analyzer (MC-FAN, Figure 1A), which was previously utilized to characterize the interaction of whole blood and plasma proteins with metal surfaces providing interesting insights into the importance of surface energy on blood coagulation mechanism [22]. Our intention is to demonstrate that the MC-FAN is a viable in vitro set-up for the study of the interaction of blood with a large class of polymers and surfaces and thus for a first-stage selection of potential blood compatible materials, avoiding the costs, the long times and the sacrifice of animals required by in vivo experiments.

Bottom Line: Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm.Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size.

View Article: PubMed Central - HTML - PubMed

Affiliation: International Centre for Young Scientists, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. c.minelli@imperial.ac.uk.

ABSTRACT

Background: Polymers are attractive materials for both biomedical engineering and cardiovascular applications. Although nano-topography has been found to influence cell behaviour, no established method exists to understand and evaluate the effects of nano-topography on polymer-blood interaction.

Results: We optimized a micro-fluidic set-up to study the interaction of whole blood with nano-structured polymer surfaces under flow conditions. Micro-fluidic chips were coated with polymethylmethacrylate films and structured by polymer demixing. Surface feature size varied from 40 nm to 400 nm and feature height from 5 nm to 50 nm. Whole blood flow rate through the micro-fluidic channels, platelet adhesion and von Willebrand factor and fibrinogen adsorption onto the structured polymer films were investigated. Whole blood flow rate through the micro-fluidic channels was found to decrease with increasing average surface feature size. Adhesion and spreading of platelets from whole blood and von Willebrand factor adsorption from platelet poor plasma were enhanced on the structured surfaces with larger feature, while fibrinogen adsorption followed the opposite trend.

Conclusion: We investigated whole blood behaviour and plasma protein adsorption on nano-structured polymer materials under flow conditions using a micro-fluidic set-up. We speculate that surface nano-topography of polymer films influences primarily plasma protein adsorption, which results in the control of platelet adhesion and thrombus formation.

No MeSH data available.


Related in: MedlinePlus