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In vivo measurement of tumor estradiol and vascular endothelial growth factor in breast cancer patients.

Garvin S, Dabrosin C - BMC Cancer (2008)

Bottom Line: Vascular endothelial growth factor (VEGF) is a potent stimulatory factor of angiogenesis and a negative prognostic indicator of breast cancer.There was a significant positive correlation between estradiol and extracellular VEGF in normal breast tissue.We conclude that VEGF and estradiol correlates significantly in normal breast tissue.

View Article: PubMed Central - HTML - PubMed

Affiliation: Linköping University, Division of Oncology, Faculty of Health Sciences, University Hospital, SE-581 85 Linköping, Sweden. stina.garvin@lio.se

ABSTRACT

Background: Angiogenesis, crucial for tumor progression, is a process regulated in the tissue micro-environment. Vascular endothelial growth factor (VEGF) is a potent stimulatory factor of angiogenesis and a negative prognostic indicator of breast cancer. VEGF is biologically active in the extracellular space and hitherto, there has been a lack of techniques enabling sampling of angiogenic molecules such as VEGF in situ. The majority of breast cancers are estrogen-dependent, and estrogen has been shown to regulate VEGF in normal breast tissue and experimental breast cancer. We investigated if microdialysis may be applicable in human breast cancer for sampling of extracellular VEGF in situ and to explore if there is an association with local estradiol and VEGF levels in normal and cancerous breast tissue.

Methods: Microdialysis was used to sample VEGF and estradiol in tumors and adjacent normal breast tissue in postmenopausal breast cancer patients. VEGF and estradiol were also measured in plasma, and immunohistochemical staining for VEGF was performed on tumor sections.

Results: We show that in vivo levels of extracellular VEGF were significantly higher in breast cancer tumors than in normal adjacent breast tissue. There was a significant positive correlation between estradiol and extracellular VEGF in normal breast tissue. However, no correlation was detected between estradiol and VEGF in tumors or between tumor VEGF and plasma VEGF.

Conclusion: We conclude that VEGF and estradiol correlates significantly in normal breast tissue. Microdialysis may be used to provide novel insight in breast tumor biology and the regulation of molecules in the extracellular space of human breast tumors in vivo.

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Extracellular VEGF in normal breast and tumors of postmenopausal breast cancer patients. VEGF sampled in vivo by microdialysis was significantly higher in tumors than in adjacent normal breast tissue (p = 0.005). Each symbol represents an individual patient.
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Figure 1: Extracellular VEGF in normal breast and tumors of postmenopausal breast cancer patients. VEGF sampled in vivo by microdialysis was significantly higher in tumors than in adjacent normal breast tissue (p = 0.005). Each symbol represents an individual patient.

Mentions: Using microdialysis, levels of estradiol and extracellular VEGF were measured in tumors and in adjacent normal breast tissue in vivo. Levels of intratumoral VEGF were significantly higher than in normal breast tissue, 6.0 pg/mL [4.1–6.5] in tumors compared to 2.4 pg/mL [2,3] in breast tissue, p = 0.005, Wilcoxon signed rank test, Figure 1). Plasma levels of VEGF were 3.67 pg/mL [0.8–5.7], range 0.62–7.59 pg/mL. In 7 of the 10 patients tumor estradiol levels were higher than in normal breast tissue, however, when comparing the whole group no significant differences were found between the levels of tumor estradiol, 106 pM [72–136], and normal breast tissue estradiol, 87 pM [48–150], Figure 2. Plasma levels of estradiol were 60 pM [40–84], range 24–198 pM.


In vivo measurement of tumor estradiol and vascular endothelial growth factor in breast cancer patients.

Garvin S, Dabrosin C - BMC Cancer (2008)

Extracellular VEGF in normal breast and tumors of postmenopausal breast cancer patients. VEGF sampled in vivo by microdialysis was significantly higher in tumors than in adjacent normal breast tissue (p = 0.005). Each symbol represents an individual patient.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2279135&req=5

Figure 1: Extracellular VEGF in normal breast and tumors of postmenopausal breast cancer patients. VEGF sampled in vivo by microdialysis was significantly higher in tumors than in adjacent normal breast tissue (p = 0.005). Each symbol represents an individual patient.
Mentions: Using microdialysis, levels of estradiol and extracellular VEGF were measured in tumors and in adjacent normal breast tissue in vivo. Levels of intratumoral VEGF were significantly higher than in normal breast tissue, 6.0 pg/mL [4.1–6.5] in tumors compared to 2.4 pg/mL [2,3] in breast tissue, p = 0.005, Wilcoxon signed rank test, Figure 1). Plasma levels of VEGF were 3.67 pg/mL [0.8–5.7], range 0.62–7.59 pg/mL. In 7 of the 10 patients tumor estradiol levels were higher than in normal breast tissue, however, when comparing the whole group no significant differences were found between the levels of tumor estradiol, 106 pM [72–136], and normal breast tissue estradiol, 87 pM [48–150], Figure 2. Plasma levels of estradiol were 60 pM [40–84], range 24–198 pM.

Bottom Line: Vascular endothelial growth factor (VEGF) is a potent stimulatory factor of angiogenesis and a negative prognostic indicator of breast cancer.There was a significant positive correlation between estradiol and extracellular VEGF in normal breast tissue.We conclude that VEGF and estradiol correlates significantly in normal breast tissue.

View Article: PubMed Central - HTML - PubMed

Affiliation: Linköping University, Division of Oncology, Faculty of Health Sciences, University Hospital, SE-581 85 Linköping, Sweden. stina.garvin@lio.se

ABSTRACT

Background: Angiogenesis, crucial for tumor progression, is a process regulated in the tissue micro-environment. Vascular endothelial growth factor (VEGF) is a potent stimulatory factor of angiogenesis and a negative prognostic indicator of breast cancer. VEGF is biologically active in the extracellular space and hitherto, there has been a lack of techniques enabling sampling of angiogenic molecules such as VEGF in situ. The majority of breast cancers are estrogen-dependent, and estrogen has been shown to regulate VEGF in normal breast tissue and experimental breast cancer. We investigated if microdialysis may be applicable in human breast cancer for sampling of extracellular VEGF in situ and to explore if there is an association with local estradiol and VEGF levels in normal and cancerous breast tissue.

Methods: Microdialysis was used to sample VEGF and estradiol in tumors and adjacent normal breast tissue in postmenopausal breast cancer patients. VEGF and estradiol were also measured in plasma, and immunohistochemical staining for VEGF was performed on tumor sections.

Results: We show that in vivo levels of extracellular VEGF were significantly higher in breast cancer tumors than in normal adjacent breast tissue. There was a significant positive correlation between estradiol and extracellular VEGF in normal breast tissue. However, no correlation was detected between estradiol and VEGF in tumors or between tumor VEGF and plasma VEGF.

Conclusion: We conclude that VEGF and estradiol correlates significantly in normal breast tissue. Microdialysis may be used to provide novel insight in breast tumor biology and the regulation of molecules in the extracellular space of human breast tumors in vivo.

Show MeSH
Related in: MedlinePlus