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Vitellogenin functions as a multivalent pattern recognition receptor with an opsonic activity.

Li Z, Zhang S, Liu Q - PLoS ONE (2008)

Bottom Line: Vitellogenin (Vg), a major reproductive protein, has been associated with infection-resistant response in fish.This study shows that fish Vg plays an integrative function in regulating immunity via its pleiotropic effects on both recognizing pathogen-associated molecular patterns and promoting macrophage phagocytosis.It also supports the notion that factors normally involved in control of female reproduction are associated with immunity in organisms that rely on Vg for oocyte development.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Biology, Ocean University of China, Qingdao, People's Republic of China.

ABSTRACT

Background: Vitellogenin (Vg), a major reproductive protein, has been associated with infection-resistant response in fish. However, the underlying mechanisms by which Vg is involved in anti-infectious response are not understood.

Methodology/results: By both protein-microbe interaction analysis and enzyme-linked immunosorbent assay as well as phagocytosis test, we demonstrate for the first time that fish Vg acts as a pattern recognition molecule with multiple specificities that can recognize bacteria as well as fungus rather than self components from fish, and functions as an opsonin that can enhance macrophage phagocytosis.

Conclusions: This study shows that fish Vg plays an integrative function in regulating immunity via its pleiotropic effects on both recognizing pathogen-associated molecular patterns and promoting macrophage phagocytosis. It also supports the notion that factors normally involved in control of female reproduction are associated with immunity in organisms that rely on Vg for oocyte development.

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Binding of FITC-labeled Vg to macrophages.Macrophage suspensions and FITC-labeled Vg were incubated together at room temperature for 30 min, and then 60 µl of cell suspensions was sampled to make smears for microscopic examination under an Olympus BX51 fluorescence microscope. For control, red blood cells were used, and treated similarly. A and C are the images under a fluorescent field, and B and D are the images under a bright light field. (A and B) Macrophages. (C and D) Red blood cells. Scale bars: 10 µm.
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pone-0001940-g006: Binding of FITC-labeled Vg to macrophages.Macrophage suspensions and FITC-labeled Vg were incubated together at room temperature for 30 min, and then 60 µl of cell suspensions was sampled to make smears for microscopic examination under an Olympus BX51 fluorescence microscope. For control, red blood cells were used, and treated similarly. A and C are the images under a fluorescent field, and B and D are the images under a bright light field. (A and B) Macrophages. (C and D) Red blood cells. Scale bars: 10 µm.

Mentions: To test if Vg can preferentially bind to macrophages, FITC-labeled Vg was incubated with the macrophages and red blood cells, respectively. It was found that Vg bound to the surface of macrophages, but not red blood cells (Fig. 6), suggesting that Vg is capable of binding to macrophage surfaces specifically.


Vitellogenin functions as a multivalent pattern recognition receptor with an opsonic activity.

Li Z, Zhang S, Liu Q - PLoS ONE (2008)

Binding of FITC-labeled Vg to macrophages.Macrophage suspensions and FITC-labeled Vg were incubated together at room temperature for 30 min, and then 60 µl of cell suspensions was sampled to make smears for microscopic examination under an Olympus BX51 fluorescence microscope. For control, red blood cells were used, and treated similarly. A and C are the images under a fluorescent field, and B and D are the images under a bright light field. (A and B) Macrophages. (C and D) Red blood cells. Scale bars: 10 µm.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2277463&req=5

pone-0001940-g006: Binding of FITC-labeled Vg to macrophages.Macrophage suspensions and FITC-labeled Vg were incubated together at room temperature for 30 min, and then 60 µl of cell suspensions was sampled to make smears for microscopic examination under an Olympus BX51 fluorescence microscope. For control, red blood cells were used, and treated similarly. A and C are the images under a fluorescent field, and B and D are the images under a bright light field. (A and B) Macrophages. (C and D) Red blood cells. Scale bars: 10 µm.
Mentions: To test if Vg can preferentially bind to macrophages, FITC-labeled Vg was incubated with the macrophages and red blood cells, respectively. It was found that Vg bound to the surface of macrophages, but not red blood cells (Fig. 6), suggesting that Vg is capable of binding to macrophage surfaces specifically.

Bottom Line: Vitellogenin (Vg), a major reproductive protein, has been associated with infection-resistant response in fish.This study shows that fish Vg plays an integrative function in regulating immunity via its pleiotropic effects on both recognizing pathogen-associated molecular patterns and promoting macrophage phagocytosis.It also supports the notion that factors normally involved in control of female reproduction are associated with immunity in organisms that rely on Vg for oocyte development.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Biology, Ocean University of China, Qingdao, People's Republic of China.

ABSTRACT

Background: Vitellogenin (Vg), a major reproductive protein, has been associated with infection-resistant response in fish. However, the underlying mechanisms by which Vg is involved in anti-infectious response are not understood.

Methodology/results: By both protein-microbe interaction analysis and enzyme-linked immunosorbent assay as well as phagocytosis test, we demonstrate for the first time that fish Vg acts as a pattern recognition molecule with multiple specificities that can recognize bacteria as well as fungus rather than self components from fish, and functions as an opsonin that can enhance macrophage phagocytosis.

Conclusions: This study shows that fish Vg plays an integrative function in regulating immunity via its pleiotropic effects on both recognizing pathogen-associated molecular patterns and promoting macrophage phagocytosis. It also supports the notion that factors normally involved in control of female reproduction are associated with immunity in organisms that rely on Vg for oocyte development.

Show MeSH