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Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia.

Delgado S, Erickson BR, Agudo R, Blair PJ, Vallejo E, Albariño CG, Vargas J, Comer JA, Rollin PE, Ksiazek TG, Olson JG, Nichol ST - PLoS Pathog. (2008)

Bottom Line: A small focus of hemorrhagic fever (HF) cases occurred near Cochabamba, Bolivia, in December 2003 and January 2004.Specimens were available from only one fatal case, which had a clinical course that included fever, headache, arthralgia, myalgia, and vomiting with subsequent deterioration and multiple hemorrhagic signs.In conclusion, two different arenaviruses, Machupo and Chapare, can be associated with severe HF cases in Bolivia.

View Article: PubMed Central - PubMed

Affiliation: Centro de Salud de Eterazama, Cochabamba, Bolivia.

ABSTRACT
A small focus of hemorrhagic fever (HF) cases occurred near Cochabamba, Bolivia, in December 2003 and January 2004. Specimens were available from only one fatal case, which had a clinical course that included fever, headache, arthralgia, myalgia, and vomiting with subsequent deterioration and multiple hemorrhagic signs. A non-cytopathic virus was isolated from two of the patient serum samples, and identified as an arenavirus by IFA staining with a rabbit polyvalent antiserum raised against South American arenaviruses known to be associated with HF (Guanarito, Machupo, and Sabiá). RT-PCR analysis and subsequent analysis of the complete virus S and L RNA segment sequences identified the virus as a member of the New World Clade B arenaviruses, which includes all the pathogenic South American arenaviruses. The virus was shown to be most closely related to Sabiá virus, but with 26% and 30% nucleotide difference in the S and L segments, and 26%, 28%, 15% and 22% amino acid differences for the L, Z, N, and GP proteins, respectively, indicating the virus represents a newly discovered arenavirus, for which we propose the name Chapare virus. In conclusion, two different arenaviruses, Machupo and Chapare, can be associated with severe HF cases in Bolivia.

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Related in: MedlinePlus

Phylogenetic analysis of the complete S and L RNA segments of New World arenaviruses.Complete S and L segments for New World arenaviruses were analyzed by Bayesian inference of phylogeny (MrBayes3.1.2) using the sequence of Pichindé virus as the outgroup. Multiple strains are grouped with small brackets and large brackets group the arenavirus Clades: A, A/Rec, B, and C. The Genbank accession numbers for the S segment analysis include: Allpahuayo (AY012686), Amapari (AF485256), Bear Canyon (AY924392), Catarina (DQ865245), Chapare (EU260463), Cupixi (AF512832), Flexal (AF485257), Guanarito (NC_005077), Junín (AY619641, NC_005081, AY746353), Latino (AF512830), Machupo (AY924208, AY619645, AY924202, NC_005078), Oliveros (U34248), Paraná (AF485261), Pichindé (NC_006447), Pirital (NC_005894), Sabiá (NC_006317), Tacaribe (NC_004293), Tamiami (AF485263), and Whitewater Arroyo (AF485264). The Genbank accession numbers used for the L segment analysis include: Allpahuayo (NC_010249), Amapari (AY924389), Bear Canyon (AY924390), Chapare (EU260464), Cupixi (NC_010252), Guanarito (NC_005082), Junín (NC_005080, AY819707, AY619640), Machupo (AY624354, NC_005079, AY619644), Oliveros (NC_010250), Pichindé (NC_006439), Pirital (NC_005897), Sabiá (NC_006313), Tacaribe (NC_004292), Tamiami (AY924393), and Whitewater Arroyo (AY924395).
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ppat-1000047-g002: Phylogenetic analysis of the complete S and L RNA segments of New World arenaviruses.Complete S and L segments for New World arenaviruses were analyzed by Bayesian inference of phylogeny (MrBayes3.1.2) using the sequence of Pichindé virus as the outgroup. Multiple strains are grouped with small brackets and large brackets group the arenavirus Clades: A, A/Rec, B, and C. The Genbank accession numbers for the S segment analysis include: Allpahuayo (AY012686), Amapari (AF485256), Bear Canyon (AY924392), Catarina (DQ865245), Chapare (EU260463), Cupixi (AF512832), Flexal (AF485257), Guanarito (NC_005077), Junín (AY619641, NC_005081, AY746353), Latino (AF512830), Machupo (AY924208, AY619645, AY924202, NC_005078), Oliveros (U34248), Paraná (AF485261), Pichindé (NC_006447), Pirital (NC_005894), Sabiá (NC_006317), Tacaribe (NC_004293), Tamiami (AF485263), and Whitewater Arroyo (AF485264). The Genbank accession numbers used for the L segment analysis include: Allpahuayo (NC_010249), Amapari (AY924389), Bear Canyon (AY924390), Chapare (EU260464), Cupixi (NC_010252), Guanarito (NC_005082), Junín (NC_005080, AY819707, AY619640), Machupo (AY624354, NC_005079, AY619644), Oliveros (NC_010250), Pichindé (NC_006439), Pirital (NC_005897), Sabiá (NC_006313), Tacaribe (NC_004292), Tamiami (AY924393), and Whitewater Arroyo (AY924395).

Mentions: Chapare virus was found to be monophyletic with Sabiá virus on phylogenetic analysis of the nucleotide or encoded amino acid sequences of the complete S or L segment (Figure 2), or NP, GP, L or Z ORFs (data not shown). No evidence of reassortment or recombination between Chapare virus and other arenaviruses was found. There is no overall change in the structure of the trees except for the previously described [4],[5] switch of the Clade A/Rec viruses from Clade A for the NP gene to Clade B for the GP gene (data not shown).


Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia.

Delgado S, Erickson BR, Agudo R, Blair PJ, Vallejo E, Albariño CG, Vargas J, Comer JA, Rollin PE, Ksiazek TG, Olson JG, Nichol ST - PLoS Pathog. (2008)

Phylogenetic analysis of the complete S and L RNA segments of New World arenaviruses.Complete S and L segments for New World arenaviruses were analyzed by Bayesian inference of phylogeny (MrBayes3.1.2) using the sequence of Pichindé virus as the outgroup. Multiple strains are grouped with small brackets and large brackets group the arenavirus Clades: A, A/Rec, B, and C. The Genbank accession numbers for the S segment analysis include: Allpahuayo (AY012686), Amapari (AF485256), Bear Canyon (AY924392), Catarina (DQ865245), Chapare (EU260463), Cupixi (AF512832), Flexal (AF485257), Guanarito (NC_005077), Junín (AY619641, NC_005081, AY746353), Latino (AF512830), Machupo (AY924208, AY619645, AY924202, NC_005078), Oliveros (U34248), Paraná (AF485261), Pichindé (NC_006447), Pirital (NC_005894), Sabiá (NC_006317), Tacaribe (NC_004293), Tamiami (AF485263), and Whitewater Arroyo (AF485264). The Genbank accession numbers used for the L segment analysis include: Allpahuayo (NC_010249), Amapari (AY924389), Bear Canyon (AY924390), Chapare (EU260464), Cupixi (NC_010252), Guanarito (NC_005082), Junín (NC_005080, AY819707, AY619640), Machupo (AY624354, NC_005079, AY619644), Oliveros (NC_010250), Pichindé (NC_006439), Pirital (NC_005897), Sabiá (NC_006313), Tacaribe (NC_004292), Tamiami (AY924393), and Whitewater Arroyo (AY924395).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2277458&req=5

ppat-1000047-g002: Phylogenetic analysis of the complete S and L RNA segments of New World arenaviruses.Complete S and L segments for New World arenaviruses were analyzed by Bayesian inference of phylogeny (MrBayes3.1.2) using the sequence of Pichindé virus as the outgroup. Multiple strains are grouped with small brackets and large brackets group the arenavirus Clades: A, A/Rec, B, and C. The Genbank accession numbers for the S segment analysis include: Allpahuayo (AY012686), Amapari (AF485256), Bear Canyon (AY924392), Catarina (DQ865245), Chapare (EU260463), Cupixi (AF512832), Flexal (AF485257), Guanarito (NC_005077), Junín (AY619641, NC_005081, AY746353), Latino (AF512830), Machupo (AY924208, AY619645, AY924202, NC_005078), Oliveros (U34248), Paraná (AF485261), Pichindé (NC_006447), Pirital (NC_005894), Sabiá (NC_006317), Tacaribe (NC_004293), Tamiami (AF485263), and Whitewater Arroyo (AF485264). The Genbank accession numbers used for the L segment analysis include: Allpahuayo (NC_010249), Amapari (AY924389), Bear Canyon (AY924390), Chapare (EU260464), Cupixi (NC_010252), Guanarito (NC_005082), Junín (NC_005080, AY819707, AY619640), Machupo (AY624354, NC_005079, AY619644), Oliveros (NC_010250), Pichindé (NC_006439), Pirital (NC_005897), Sabiá (NC_006313), Tacaribe (NC_004292), Tamiami (AY924393), and Whitewater Arroyo (AY924395).
Mentions: Chapare virus was found to be monophyletic with Sabiá virus on phylogenetic analysis of the nucleotide or encoded amino acid sequences of the complete S or L segment (Figure 2), or NP, GP, L or Z ORFs (data not shown). No evidence of reassortment or recombination between Chapare virus and other arenaviruses was found. There is no overall change in the structure of the trees except for the previously described [4],[5] switch of the Clade A/Rec viruses from Clade A for the NP gene to Clade B for the GP gene (data not shown).

Bottom Line: A small focus of hemorrhagic fever (HF) cases occurred near Cochabamba, Bolivia, in December 2003 and January 2004.Specimens were available from only one fatal case, which had a clinical course that included fever, headache, arthralgia, myalgia, and vomiting with subsequent deterioration and multiple hemorrhagic signs.In conclusion, two different arenaviruses, Machupo and Chapare, can be associated with severe HF cases in Bolivia.

View Article: PubMed Central - PubMed

Affiliation: Centro de Salud de Eterazama, Cochabamba, Bolivia.

ABSTRACT
A small focus of hemorrhagic fever (HF) cases occurred near Cochabamba, Bolivia, in December 2003 and January 2004. Specimens were available from only one fatal case, which had a clinical course that included fever, headache, arthralgia, myalgia, and vomiting with subsequent deterioration and multiple hemorrhagic signs. A non-cytopathic virus was isolated from two of the patient serum samples, and identified as an arenavirus by IFA staining with a rabbit polyvalent antiserum raised against South American arenaviruses known to be associated with HF (Guanarito, Machupo, and Sabiá). RT-PCR analysis and subsequent analysis of the complete virus S and L RNA segment sequences identified the virus as a member of the New World Clade B arenaviruses, which includes all the pathogenic South American arenaviruses. The virus was shown to be most closely related to Sabiá virus, but with 26% and 30% nucleotide difference in the S and L segments, and 26%, 28%, 15% and 22% amino acid differences for the L, Z, N, and GP proteins, respectively, indicating the virus represents a newly discovered arenavirus, for which we propose the name Chapare virus. In conclusion, two different arenaviruses, Machupo and Chapare, can be associated with severe HF cases in Bolivia.

Show MeSH
Related in: MedlinePlus