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Mouse mammary tumor virus (MMTV)-like DNA sequences in the breast tumors of father, mother, and daughter.

Etkind PR, Stewart AF, Wiernik PH - Infect. Agents Cancer (2008)

Bottom Line: MMTV-like envelope (env) and long terminal repeat (LTR) sequences containing the MMTV superantigen gene (sag) were detected in the malignant tissues of all three family members.The amplified LTR sequences containing sag sequences segregated to specific branches of the MMTV phylogenetic tree and did not form a distinct branch of their own.The presence of MMTV-like DNA sequences in the malignant tissues of all three family members suggests the possibility of MMTV as an etiological agent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Our Lady of Mercy Medical Center-Comprehensive Cancer Center, New York Medical College, Bronx, New York, USA. petkind@olmhs.org

ABSTRACT

Background: The diagnosis of late onset breast cancer in a father, mother, and daughter living in the same house for decades suggested the possibility of an environmental agent as a common etiological factor. Both molecular and epidemiological data have indicated a possible role for the mouse mammary tumor virus (MMTV), the etiological agent of breast cancer in mice, in a certain percentage of human breast tumors. The aim of this study was to determine if MMTV might be involved in the breast cancer of this cluster of three family members.

Results: MMTV-like envelope (env) and long terminal repeat (LTR) sequences containing the MMTV superantigen gene (sag) were detected in the malignant tissues of all three family members. The amplified env gene sequences were 98.0%-99.6% homologous to the MMTV env sequences found in the GR, C3H, and BR6 mouse strains. The amplified LTR sequences containing sag sequences segregated to specific branches of the MMTV phylogenetic tree and did not form a distinct branch of their own.

Conclusion: The presence of MMTV-like DNA sequences in the malignant tissues of all three family members suggests the possibility of MMTV as an etiological agent. Phylogenetic data suggest that the MMTV-like DNA sequences are mouse and not human derived and that the ultimate reservoir of MMTV is most likely the mouse. Although the route by which these family members came to be infected with MMTV is unknown, the possibility exists that such infection may have resulted from a shared exposure to mice.

No MeSH data available.


Related in: MedlinePlus

Sequence homology of the highly variable COOH-terminal region of the MMTV sag gene of MMTV proviruses Mtv-1 [22,23] and Mtv-8 [20,21] to the amplified highly variable MMTV sag sequences cloned from the primary breast tumor tissue of the mother and daughter and metastatic breast tumor tissue in lymph node of the father. Clones are named as M (mother), D (daughter), and F (father) followed by a dash and the letter s for sag and numbers (1–4) identifying the order in which they were cloned. - denotes the same nucleotide.
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Figure 4: Sequence homology of the highly variable COOH-terminal region of the MMTV sag gene of MMTV proviruses Mtv-1 [22,23] and Mtv-8 [20,21] to the amplified highly variable MMTV sag sequences cloned from the primary breast tumor tissue of the mother and daughter and metastatic breast tumor tissue in lymph node of the father. Clones are named as M (mother), D (daughter), and F (father) followed by a dash and the letter s for sag and numbers (1–4) identifying the order in which they were cloned. - denotes the same nucleotide.

Mentions: In the conserved regions of the LTR ORF all 12 clones shared 96–98 percent homology with numerous strains of MMTV and with each other. However, within the highly variable COOH terminus of the sag gene all of the 12 isolated clones were either identical or nearly identical to either the MMTV proviral sequence Mtv-8 [20,21] or Mtv-1 [22,23] as shown in Figure 4. Each of the three family members contained COOH-terminal sag sequences that were identical or nearly identical (one or two base differences) to one of these two proviral sequences.


Mouse mammary tumor virus (MMTV)-like DNA sequences in the breast tumors of father, mother, and daughter.

Etkind PR, Stewart AF, Wiernik PH - Infect. Agents Cancer (2008)

Sequence homology of the highly variable COOH-terminal region of the MMTV sag gene of MMTV proviruses Mtv-1 [22,23] and Mtv-8 [20,21] to the amplified highly variable MMTV sag sequences cloned from the primary breast tumor tissue of the mother and daughter and metastatic breast tumor tissue in lymph node of the father. Clones are named as M (mother), D (daughter), and F (father) followed by a dash and the letter s for sag and numbers (1–4) identifying the order in which they were cloned. - denotes the same nucleotide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2277433&req=5

Figure 4: Sequence homology of the highly variable COOH-terminal region of the MMTV sag gene of MMTV proviruses Mtv-1 [22,23] and Mtv-8 [20,21] to the amplified highly variable MMTV sag sequences cloned from the primary breast tumor tissue of the mother and daughter and metastatic breast tumor tissue in lymph node of the father. Clones are named as M (mother), D (daughter), and F (father) followed by a dash and the letter s for sag and numbers (1–4) identifying the order in which they were cloned. - denotes the same nucleotide.
Mentions: In the conserved regions of the LTR ORF all 12 clones shared 96–98 percent homology with numerous strains of MMTV and with each other. However, within the highly variable COOH terminus of the sag gene all of the 12 isolated clones were either identical or nearly identical to either the MMTV proviral sequence Mtv-8 [20,21] or Mtv-1 [22,23] as shown in Figure 4. Each of the three family members contained COOH-terminal sag sequences that were identical or nearly identical (one or two base differences) to one of these two proviral sequences.

Bottom Line: MMTV-like envelope (env) and long terminal repeat (LTR) sequences containing the MMTV superantigen gene (sag) were detected in the malignant tissues of all three family members.The amplified LTR sequences containing sag sequences segregated to specific branches of the MMTV phylogenetic tree and did not form a distinct branch of their own.The presence of MMTV-like DNA sequences in the malignant tissues of all three family members suggests the possibility of MMTV as an etiological agent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Our Lady of Mercy Medical Center-Comprehensive Cancer Center, New York Medical College, Bronx, New York, USA. petkind@olmhs.org

ABSTRACT

Background: The diagnosis of late onset breast cancer in a father, mother, and daughter living in the same house for decades suggested the possibility of an environmental agent as a common etiological factor. Both molecular and epidemiological data have indicated a possible role for the mouse mammary tumor virus (MMTV), the etiological agent of breast cancer in mice, in a certain percentage of human breast tumors. The aim of this study was to determine if MMTV might be involved in the breast cancer of this cluster of three family members.

Results: MMTV-like envelope (env) and long terminal repeat (LTR) sequences containing the MMTV superantigen gene (sag) were detected in the malignant tissues of all three family members. The amplified env gene sequences were 98.0%-99.6% homologous to the MMTV env sequences found in the GR, C3H, and BR6 mouse strains. The amplified LTR sequences containing sag sequences segregated to specific branches of the MMTV phylogenetic tree and did not form a distinct branch of their own.

Conclusion: The presence of MMTV-like DNA sequences in the malignant tissues of all three family members suggests the possibility of MMTV as an etiological agent. Phylogenetic data suggest that the MMTV-like DNA sequences are mouse and not human derived and that the ultimate reservoir of MMTV is most likely the mouse. Although the route by which these family members came to be infected with MMTV is unknown, the possibility exists that such infection may have resulted from a shared exposure to mice.

No MeSH data available.


Related in: MedlinePlus