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Mouse mammary tumor virus (MMTV)-like DNA sequences in the breast tumors of father, mother, and daughter.

Etkind PR, Stewart AF, Wiernik PH - Infect. Agents Cancer (2008)

Bottom Line: MMTV-like envelope (env) and long terminal repeat (LTR) sequences containing the MMTV superantigen gene (sag) were detected in the malignant tissues of all three family members.The amplified LTR sequences containing sag sequences segregated to specific branches of the MMTV phylogenetic tree and did not form a distinct branch of their own.The presence of MMTV-like DNA sequences in the malignant tissues of all three family members suggests the possibility of MMTV as an etiological agent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Our Lady of Mercy Medical Center-Comprehensive Cancer Center, New York Medical College, Bronx, New York, USA. petkind@olmhs.org

ABSTRACT

Background: The diagnosis of late onset breast cancer in a father, mother, and daughter living in the same house for decades suggested the possibility of an environmental agent as a common etiological factor. Both molecular and epidemiological data have indicated a possible role for the mouse mammary tumor virus (MMTV), the etiological agent of breast cancer in mice, in a certain percentage of human breast tumors. The aim of this study was to determine if MMTV might be involved in the breast cancer of this cluster of three family members.

Results: MMTV-like envelope (env) and long terminal repeat (LTR) sequences containing the MMTV superantigen gene (sag) were detected in the malignant tissues of all three family members. The amplified env gene sequences were 98.0%-99.6% homologous to the MMTV env sequences found in the GR, C3H, and BR6 mouse strains. The amplified LTR sequences containing sag sequences segregated to specific branches of the MMTV phylogenetic tree and did not form a distinct branch of their own.

Conclusion: The presence of MMTV-like DNA sequences in the malignant tissues of all three family members suggests the possibility of MMTV as an etiological agent. Phylogenetic data suggest that the MMTV-like DNA sequences are mouse and not human derived and that the ultimate reservoir of MMTV is most likely the mouse. Although the route by which these family members came to be infected with MMTV is unknown, the possibility exists that such infection may have resulted from a shared exposure to mice.

No MeSH data available.


Related in: MedlinePlus

Sequences of the 250 bp PCR MMTV-like env gene product amplified from the DNA of primary breast cancer tissue of mother and daughter and metastatic breast tumor tissue in lymph node of father compared with the sequences in this region of the env gene of GR, C3H and BR6 strains of MMTV. The numbers 1, 2 and 3 indicate the three locations where the BR6 strain differs from the GR and C3H strains in this region of the MMTV env gene [17,18,19]. The A and ↓ indicate the location at which the identical single base change described in the text occurs. The numbers 7656 and 7905 indicate the location of the MMTV 250 bp env gene sequence within the MMTV genome [17]. Clones are designated as M (mother), D (daughter), and F (father) followed by a number (1–4) denoting the order in which they were cloned. - denotes the same nucleotide.
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Figure 3: Sequences of the 250 bp PCR MMTV-like env gene product amplified from the DNA of primary breast cancer tissue of mother and daughter and metastatic breast tumor tissue in lymph node of father compared with the sequences in this region of the env gene of GR, C3H and BR6 strains of MMTV. The numbers 1, 2 and 3 indicate the three locations where the BR6 strain differs from the GR and C3H strains in this region of the MMTV env gene [17,18,19]. The A and ↓ indicate the location at which the identical single base change described in the text occurs. The numbers 7656 and 7905 indicate the location of the MMTV 250 bp env gene sequence within the MMTV genome [17]. Clones are designated as M (mother), D (daughter), and F (father) followed by a number (1–4) denoting the order in which they were cloned. - denotes the same nucleotide.

Mentions: The amplified MMTV env gene-specific 250-bp sequences present in the primary and metastatic breast tumor tissue were cloned using the Invitrogen TOPO TA Cloning kit for Sequencing. A total of 12 clones (4 for each family member) were sequenced. The sequences of the 12 amplified fragments were shown to be 98.0% – 99.6 percent homologous to the GR, C3H and BR6 mouse strains of MMTV in this 250-bp region of the MMTV env gene. Figure 3 shows the comparison of the sequences of the 12 clones to the three strains of MMTV [17-19] and to each other in this region of the MMTV env gene.


Mouse mammary tumor virus (MMTV)-like DNA sequences in the breast tumors of father, mother, and daughter.

Etkind PR, Stewart AF, Wiernik PH - Infect. Agents Cancer (2008)

Sequences of the 250 bp PCR MMTV-like env gene product amplified from the DNA of primary breast cancer tissue of mother and daughter and metastatic breast tumor tissue in lymph node of father compared with the sequences in this region of the env gene of GR, C3H and BR6 strains of MMTV. The numbers 1, 2 and 3 indicate the three locations where the BR6 strain differs from the GR and C3H strains in this region of the MMTV env gene [17,18,19]. The A and ↓ indicate the location at which the identical single base change described in the text occurs. The numbers 7656 and 7905 indicate the location of the MMTV 250 bp env gene sequence within the MMTV genome [17]. Clones are designated as M (mother), D (daughter), and F (father) followed by a number (1–4) denoting the order in which they were cloned. - denotes the same nucleotide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2277433&req=5

Figure 3: Sequences of the 250 bp PCR MMTV-like env gene product amplified from the DNA of primary breast cancer tissue of mother and daughter and metastatic breast tumor tissue in lymph node of father compared with the sequences in this region of the env gene of GR, C3H and BR6 strains of MMTV. The numbers 1, 2 and 3 indicate the three locations where the BR6 strain differs from the GR and C3H strains in this region of the MMTV env gene [17,18,19]. The A and ↓ indicate the location at which the identical single base change described in the text occurs. The numbers 7656 and 7905 indicate the location of the MMTV 250 bp env gene sequence within the MMTV genome [17]. Clones are designated as M (mother), D (daughter), and F (father) followed by a number (1–4) denoting the order in which they were cloned. - denotes the same nucleotide.
Mentions: The amplified MMTV env gene-specific 250-bp sequences present in the primary and metastatic breast tumor tissue were cloned using the Invitrogen TOPO TA Cloning kit for Sequencing. A total of 12 clones (4 for each family member) were sequenced. The sequences of the 12 amplified fragments were shown to be 98.0% – 99.6 percent homologous to the GR, C3H and BR6 mouse strains of MMTV in this 250-bp region of the MMTV env gene. Figure 3 shows the comparison of the sequences of the 12 clones to the three strains of MMTV [17-19] and to each other in this region of the MMTV env gene.

Bottom Line: MMTV-like envelope (env) and long terminal repeat (LTR) sequences containing the MMTV superantigen gene (sag) were detected in the malignant tissues of all three family members.The amplified LTR sequences containing sag sequences segregated to specific branches of the MMTV phylogenetic tree and did not form a distinct branch of their own.The presence of MMTV-like DNA sequences in the malignant tissues of all three family members suggests the possibility of MMTV as an etiological agent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Our Lady of Mercy Medical Center-Comprehensive Cancer Center, New York Medical College, Bronx, New York, USA. petkind@olmhs.org

ABSTRACT

Background: The diagnosis of late onset breast cancer in a father, mother, and daughter living in the same house for decades suggested the possibility of an environmental agent as a common etiological factor. Both molecular and epidemiological data have indicated a possible role for the mouse mammary tumor virus (MMTV), the etiological agent of breast cancer in mice, in a certain percentage of human breast tumors. The aim of this study was to determine if MMTV might be involved in the breast cancer of this cluster of three family members.

Results: MMTV-like envelope (env) and long terminal repeat (LTR) sequences containing the MMTV superantigen gene (sag) were detected in the malignant tissues of all three family members. The amplified env gene sequences were 98.0%-99.6% homologous to the MMTV env sequences found in the GR, C3H, and BR6 mouse strains. The amplified LTR sequences containing sag sequences segregated to specific branches of the MMTV phylogenetic tree and did not form a distinct branch of their own.

Conclusion: The presence of MMTV-like DNA sequences in the malignant tissues of all three family members suggests the possibility of MMTV as an etiological agent. Phylogenetic data suggest that the MMTV-like DNA sequences are mouse and not human derived and that the ultimate reservoir of MMTV is most likely the mouse. Although the route by which these family members came to be infected with MMTV is unknown, the possibility exists that such infection may have resulted from a shared exposure to mice.

No MeSH data available.


Related in: MedlinePlus