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Characterisation of Shiga toxin-producing Escherichia coli O157 strains isolated from humans in Argentina, Australia and New Zealand.

Leotta GA, Miliwebsky ES, Chinen I, Espinosa EM, Azzopardi K, Tennant SM, Robins-Browne RM, Rivas M - BMC Microbiol. (2008)

Bottom Line: In Australia, on the other hand, STEC O157:H7 is associated with a minority of HUS cases.STEC O157 strains isolated in Argentina, Australia, and New Zealand differed from each other in terms of stx-genotype and phage type.International collaborative studies of the type reported here are needed to detect and monitor potentially hypervirulent STEC clones.

View Article: PubMed Central - HTML - PubMed

Affiliation: Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas - ANLIS Dr. Carlos G. Malbrán. Av. Vélez Sarsfield 563 (1281) Ciudad Autónoma de Buenos Aires, Argentina.galeotta@anlis.gov.ar

ABSTRACT

Background: Shiga toxin-producing Escherichia coli (STEC) is an important cause of bloody diarrhoea (BD), non-bloody diarrhoea (NBD) and the haemolytic uraemic syndrome (HUS). In Argentina and New Zealand, the most prevalent STEC serotype is O157:H7, which is responsible for the majority of HUS cases. In Australia, on the other hand, STEC O157:H7 is associated with a minority of HUS cases. The main aims of this study were to compare the phenotypic and genotypic characteristics of STEC O157 strains isolated between 1993 and 1996 from humans in Argentina, Australia and New Zealand, and to establish their clonal relatedness.

Results: Seventy-three O157 STEC strains, isolated from HUS (n = 36), BD (n = 20), NBD (n = 10), or unspecified conditions (n = 7) in Argentina, Australia and New Zealand, were analysed. The strains were confirmed to be E. coli O157 by biochemical tests and serotyping. A multiplex polymerase chain reaction (PCR) was used to amplify the stx1, stx2 and rfbO157 genes and a genotyping method based on PCR-RFLP was used to determine stx1 and stx2 variants. This analysis revealed that the most frequent stx genotypes were stx2/stx2c (vh-a) (91%) in Argentina, stx2 (89%) in New Zealand, and stx1/stx2 (30%) in Australia. No stx1-postive strains were identified in Argentina or New Zealand. All strains harboured the eae gene and 72 strains produced enterohaemolysin (EHEC-Hly). The clonal relatedness of strains was investigated by phage typing and pulsed-field gel electrophoresis (PFGE). The most frequent phage types (PT) identified in Argentinian, Australian, and New Zealand strains were PT49 (n = 12), PT14 (n = 9), and PT2 (n = 15), respectively. Forty-six different patterns were obtained by XbaI-PFGE; 37 strains were grouped in 10 clusters and 36 strains showed unique patterns. Most clusters could be further subdivided by BlnI-PFGE.

Conclusion: STEC O157 strains isolated in Argentina, Australia, and New Zealand differed from each other in terms of stx-genotype and phage type. Additionally, no common PFGE patterns were found in strains isolated in the three countries. International collaborative studies of the type reported here are needed to detect and monitor potentially hypervirulent STEC clones.

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Clonal relationship of 73 STEC O157 strains isolated between 1993 and 1996 from humans in Argentina, Australia, and New Zealand.
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Figure 1: Clonal relationship of 73 STEC O157 strains isolated between 1993 and 1996 from humans in Argentina, Australia, and New Zealand.

Mentions: Ten clusters contained isolates with indistinguishable XbaI-PFGE profiles (Figure 1). These were cluster I (3 strains from Argentina); cluster II (3 strains from Australia); cluster III (2 strains from Argentina); cluster IV (5 strains from Argentina); cluster V (2 strains from New Zealand); cluster VI (8 strains from Argentina); cluster VII (2 strains from New Zealand); cluster VIII (4 strains from New Zealand); cluster IX (3 strains from New Zealand); and cluster X (5 strains from Australia). To refine the cluster analysis further, we determined the BlnI-PFGE profile of each isolate. This revealed that most strains with identical XbaI-PFGE pattern could be discerned from each other by restriction with a second enzyme (Table 5). Some clusters could also be split by using phage typing and stx genotyping (Table 5). Only two clusters, II (3 strains) and X (5 strains), both from Australia, were indistinguishable by XbaI- and BlnI-PFGE, phage typing and stx-genotyping, indicating that these were probable clones.


Characterisation of Shiga toxin-producing Escherichia coli O157 strains isolated from humans in Argentina, Australia and New Zealand.

Leotta GA, Miliwebsky ES, Chinen I, Espinosa EM, Azzopardi K, Tennant SM, Robins-Browne RM, Rivas M - BMC Microbiol. (2008)

Clonal relationship of 73 STEC O157 strains isolated between 1993 and 1996 from humans in Argentina, Australia, and New Zealand.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2277424&req=5

Figure 1: Clonal relationship of 73 STEC O157 strains isolated between 1993 and 1996 from humans in Argentina, Australia, and New Zealand.
Mentions: Ten clusters contained isolates with indistinguishable XbaI-PFGE profiles (Figure 1). These were cluster I (3 strains from Argentina); cluster II (3 strains from Australia); cluster III (2 strains from Argentina); cluster IV (5 strains from Argentina); cluster V (2 strains from New Zealand); cluster VI (8 strains from Argentina); cluster VII (2 strains from New Zealand); cluster VIII (4 strains from New Zealand); cluster IX (3 strains from New Zealand); and cluster X (5 strains from Australia). To refine the cluster analysis further, we determined the BlnI-PFGE profile of each isolate. This revealed that most strains with identical XbaI-PFGE pattern could be discerned from each other by restriction with a second enzyme (Table 5). Some clusters could also be split by using phage typing and stx genotyping (Table 5). Only two clusters, II (3 strains) and X (5 strains), both from Australia, were indistinguishable by XbaI- and BlnI-PFGE, phage typing and stx-genotyping, indicating that these were probable clones.

Bottom Line: In Australia, on the other hand, STEC O157:H7 is associated with a minority of HUS cases.STEC O157 strains isolated in Argentina, Australia, and New Zealand differed from each other in terms of stx-genotype and phage type.International collaborative studies of the type reported here are needed to detect and monitor potentially hypervirulent STEC clones.

View Article: PubMed Central - HTML - PubMed

Affiliation: Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas - ANLIS Dr. Carlos G. Malbrán. Av. Vélez Sarsfield 563 (1281) Ciudad Autónoma de Buenos Aires, Argentina.galeotta@anlis.gov.ar

ABSTRACT

Background: Shiga toxin-producing Escherichia coli (STEC) is an important cause of bloody diarrhoea (BD), non-bloody diarrhoea (NBD) and the haemolytic uraemic syndrome (HUS). In Argentina and New Zealand, the most prevalent STEC serotype is O157:H7, which is responsible for the majority of HUS cases. In Australia, on the other hand, STEC O157:H7 is associated with a minority of HUS cases. The main aims of this study were to compare the phenotypic and genotypic characteristics of STEC O157 strains isolated between 1993 and 1996 from humans in Argentina, Australia and New Zealand, and to establish their clonal relatedness.

Results: Seventy-three O157 STEC strains, isolated from HUS (n = 36), BD (n = 20), NBD (n = 10), or unspecified conditions (n = 7) in Argentina, Australia and New Zealand, were analysed. The strains were confirmed to be E. coli O157 by biochemical tests and serotyping. A multiplex polymerase chain reaction (PCR) was used to amplify the stx1, stx2 and rfbO157 genes and a genotyping method based on PCR-RFLP was used to determine stx1 and stx2 variants. This analysis revealed that the most frequent stx genotypes were stx2/stx2c (vh-a) (91%) in Argentina, stx2 (89%) in New Zealand, and stx1/stx2 (30%) in Australia. No stx1-postive strains were identified in Argentina or New Zealand. All strains harboured the eae gene and 72 strains produced enterohaemolysin (EHEC-Hly). The clonal relatedness of strains was investigated by phage typing and pulsed-field gel electrophoresis (PFGE). The most frequent phage types (PT) identified in Argentinian, Australian, and New Zealand strains were PT49 (n = 12), PT14 (n = 9), and PT2 (n = 15), respectively. Forty-six different patterns were obtained by XbaI-PFGE; 37 strains were grouped in 10 clusters and 36 strains showed unique patterns. Most clusters could be further subdivided by BlnI-PFGE.

Conclusion: STEC O157 strains isolated in Argentina, Australia, and New Zealand differed from each other in terms of stx-genotype and phage type. Additionally, no common PFGE patterns were found in strains isolated in the three countries. International collaborative studies of the type reported here are needed to detect and monitor potentially hypervirulent STEC clones.

Show MeSH
Related in: MedlinePlus