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Polyphasic taxonomy of Aspergillus section Usti.

Houbraken J, Due M, Varga J, Meijer M, Frisvad JC, Samson RA - Stud. Mycol. (2007)

Bottom Line: A. insuetus differs from A. ustus in producing drimans and ophiobolin G and H and not producing ustic acid and austocystins.The molecular, physiological and morphological data also indicated that another species, A. keveiisp. nov. is closely related but distinct from A. insuetus.Aspergillus section Usti sensu stricto includes 8 species: A. ustus, A. puniceus, A. granulosus, A. pseudodeflectus, A. calidoustus, A. insuetus and A. keveii together with Emericella heterothallica.

View Article: PubMed Central - PubMed

Affiliation: CBS Fungal Biodiversity Centre, PO Box 85167, NL-3508 AD Utrecht, the Netherlands.

ABSTRACT
Aspergillus ustus is a very common species in foods, soil and indoor environments. Based on chemical, molecular and morphological data, A. insuetus is separated from A. ustus and revived. A. insuetus differs from A. ustus in producing drimans and ophiobolin G and H and not producing ustic acid and austocystins. The molecular, physiological and morphological data also indicated that another species, A. keveiisp. nov. is closely related but distinct from A. insuetus. Aspergillus section Usti sensu stricto includes 8 species: A. ustus, A. puniceus, A. granulosus, A. pseudodeflectus, A. calidoustus, A. insuetus and A. keveii together with Emericella heterothallica.

No MeSH data available.


Neighbour-joining tree based on β-tubulin sequence data of Aspergillus section Usti. Numbers above branches are bootstrap values. Only values above 70 % are indicated.
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fig1: Neighbour-joining tree based on β-tubulin sequence data of Aspergillus section Usti. Numbers above branches are bootstrap values. Only values above 70 % are indicated.

Mentions: For the molecular analysis, four genomic regions, the ITS region, and parts of the actin, calmodulin and β-tubulin genes were amplified and sequenced. Phylogenetic analysis of the data was carried out using the neighbour-joining technique and parsimony analysis. The trees obtained by the different approaches were identical, neighbour-joining trees based on the different data sets are shown in Figs 1, 2, 3 and 4. During analysis of part of the β-tubulin gene, 487 characters were analyzed, 111 of which were found to be parsimony informative. The topology of the tree is the same as that of one of the more than 104 maximum parsimony trees constructed by the PAUP program (length: 216 steps, consistency index: 0.8148, retention index: 0.9679). The calmodulin data set included 474 characters, with 172 parsimony informative characters (1 MP tree, tree length: 360, consistency index: 0.8083, retention index: 0.9550). The actin data set included 406 characters, with 161 parsimony informative characters (3 MP trees, tree length: 292, consistency index: 0.8870, retention index: 0.9633). The ITS data set included 482 characters, 26 of which were parsimony informative (>104 MP trees, tree length: 71, consistency index: 0.9155, retention index: 0.9781).


Polyphasic taxonomy of Aspergillus section Usti.

Houbraken J, Due M, Varga J, Meijer M, Frisvad JC, Samson RA - Stud. Mycol. (2007)

Neighbour-joining tree based on β-tubulin sequence data of Aspergillus section Usti. Numbers above branches are bootstrap values. Only values above 70 % are indicated.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2275196&req=5

fig1: Neighbour-joining tree based on β-tubulin sequence data of Aspergillus section Usti. Numbers above branches are bootstrap values. Only values above 70 % are indicated.
Mentions: For the molecular analysis, four genomic regions, the ITS region, and parts of the actin, calmodulin and β-tubulin genes were amplified and sequenced. Phylogenetic analysis of the data was carried out using the neighbour-joining technique and parsimony analysis. The trees obtained by the different approaches were identical, neighbour-joining trees based on the different data sets are shown in Figs 1, 2, 3 and 4. During analysis of part of the β-tubulin gene, 487 characters were analyzed, 111 of which were found to be parsimony informative. The topology of the tree is the same as that of one of the more than 104 maximum parsimony trees constructed by the PAUP program (length: 216 steps, consistency index: 0.8148, retention index: 0.9679). The calmodulin data set included 474 characters, with 172 parsimony informative characters (1 MP tree, tree length: 360, consistency index: 0.8083, retention index: 0.9550). The actin data set included 406 characters, with 161 parsimony informative characters (3 MP trees, tree length: 292, consistency index: 0.8870, retention index: 0.9633). The ITS data set included 482 characters, 26 of which were parsimony informative (>104 MP trees, tree length: 71, consistency index: 0.9155, retention index: 0.9781).

Bottom Line: A. insuetus differs from A. ustus in producing drimans and ophiobolin G and H and not producing ustic acid and austocystins.The molecular, physiological and morphological data also indicated that another species, A. keveiisp. nov. is closely related but distinct from A. insuetus.Aspergillus section Usti sensu stricto includes 8 species: A. ustus, A. puniceus, A. granulosus, A. pseudodeflectus, A. calidoustus, A. insuetus and A. keveii together with Emericella heterothallica.

View Article: PubMed Central - PubMed

Affiliation: CBS Fungal Biodiversity Centre, PO Box 85167, NL-3508 AD Utrecht, the Netherlands.

ABSTRACT
Aspergillus ustus is a very common species in foods, soil and indoor environments. Based on chemical, molecular and morphological data, A. insuetus is separated from A. ustus and revived. A. insuetus differs from A. ustus in producing drimans and ophiobolin G and H and not producing ustic acid and austocystins. The molecular, physiological and morphological data also indicated that another species, A. keveiisp. nov. is closely related but distinct from A. insuetus. Aspergillus section Usti sensu stricto includes 8 species: A. ustus, A. puniceus, A. granulosus, A. pseudodeflectus, A. calidoustus, A. insuetus and A. keveii together with Emericella heterothallica.

No MeSH data available.