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A Mechanistic Approach for Modulation of Arsenic Toxicity in Human Lymphocytes by Curcumin, an Active Constituent of Medicinal Herb Curcuma longa Linn.

Mukherjee S, Roy M, Dey S, Bhattacharya RK - J Clin Biochem Nutr (2007)

Bottom Line: Amelioration of such damages with natural compounds could be an effective strategy to combat arsenic toxicity.The expression of polymerase, a repair enzyme, was found to be highly elevated when arsenite induced damaged cells were allowed to repair in presence of curcumin.Results indicate that curcumin has significant role in confronting the deleterious effect caused by arsenic, which could be an economic mode of arsenic mitigation among rural population in West Bengal, India.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Carcinogenesis & Toxicology, Chittaranjan National Cancer Institute, 37 S.P. Mukherjee Road, Kolkata 700 026, India.

ABSTRACT
Chronic exposure of humans to high concentrations of arsenic in drinking water is associated with skin lesions, peripheral vascular disease, hypertension, blackfoot disease and a high risk of cancer. Arsenic induces single strand breaks, DNA-protein crosslinks and apurinic sites in DNA, which are prerequisites for induction of cancer. Amelioration of such damages with natural compounds could be an effective strategy to combat arsenic toxicity. Curcumin is the active ingredient of turmeric, a common household spice, which is a rich source of polyphenols and this compound has been extensively studied as a chemopreventive agent against many types of cancer. The present study investigates whether curcumin could counteract the DNA damage caused by arsenic as assessed by single cell gel electrophoresis (SCGE) using peripheral blood lymphocytes, from healthy donors. It was observed that DNA damage induced by arsenic could be efficiently reduced by curcumin and the effect was more pronounced when lymphocytes were pre-incubated with curcumin prior to arsenic insult. Arsenic caused DNA damage by generation of reactive oxygen species (ROS) and enhancement of lipid peroxidation levels. Curcumin counteracted the damage by quenching ROS, decreasing the level of lipid peroxidation and increasing the level of phase II detoxification enzymes like catalase, superoxide dismutase and glutathione peroxidase. Curcumin also enhanced the DNA repair activity against arsenic induced damage. The expression of polymerase, a repair enzyme, was found to be highly elevated when arsenite induced damaged cells were allowed to repair in presence of curcumin. Results indicate that curcumin has significant role in confronting the deleterious effect caused by arsenic, which could be an economic mode of arsenic mitigation among rural population in West Bengal, India.

No MeSH data available.


Related in: MedlinePlus

Inhibition of lipid peroxidation by curcumin during treatment with As III (1000 µM) in normal human lymphocytes. Cur I (10 µM), Cur II (25 µM) and Cur III (50 µM).
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Figure 4: Inhibition of lipid peroxidation by curcumin during treatment with As III (1000 µM) in normal human lymphocytes. Cur I (10 µM), Cur II (25 µM) and Cur III (50 µM).

Mentions: When the cells were analyzed for intracellular ROS production with the fluorescent dye DCFH-DA using a spectrofluorimeter, it was found that 1000 µM As III enhanced the level of ROS generation over the background level found in control cells. This increased ROS generation was effectively quenched by curcumin at different concentrations (10, 25 and 50 µM respectively). As depicted in Fig. 3 (a), arsenic exposure increases generation of ROS production three times higher than that of the control value which in presence of curcumin was effectively reduced and with 50 µM curcumin it was almost at the background level. When these arsenic treated cells were viewed under a fluorescence microscope, very intense fluorescence compared to the background was observed indicating generation of ROS; presence of curcumin appreciably reduced the fluorescence intensity indicating quenching action of curcumin. Photographs showing generation of ROS by arsenite and its quenching by curcumin are shown in Fig. 3 (b). Curcumin was also proved effective in inhibiting the lipid peroxidation caused by As III (Fig. 4). As represented in the figure the levels of TBARS were significantly increased from 100% in control lymphocytes (without any treatment) to 153% in cells treated with As 1000 µM. Administration of curcumin during this arsenite treatment significantly decreased the levels of TBARS by 84.6%, 72% and 52% in presence of 10, 25 and 50 µM respectively.


A Mechanistic Approach for Modulation of Arsenic Toxicity in Human Lymphocytes by Curcumin, an Active Constituent of Medicinal Herb Curcuma longa Linn.

Mukherjee S, Roy M, Dey S, Bhattacharya RK - J Clin Biochem Nutr (2007)

Inhibition of lipid peroxidation by curcumin during treatment with As III (1000 µM) in normal human lymphocytes. Cur I (10 µM), Cur II (25 µM) and Cur III (50 µM).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2274986&req=5

Figure 4: Inhibition of lipid peroxidation by curcumin during treatment with As III (1000 µM) in normal human lymphocytes. Cur I (10 µM), Cur II (25 µM) and Cur III (50 µM).
Mentions: When the cells were analyzed for intracellular ROS production with the fluorescent dye DCFH-DA using a spectrofluorimeter, it was found that 1000 µM As III enhanced the level of ROS generation over the background level found in control cells. This increased ROS generation was effectively quenched by curcumin at different concentrations (10, 25 and 50 µM respectively). As depicted in Fig. 3 (a), arsenic exposure increases generation of ROS production three times higher than that of the control value which in presence of curcumin was effectively reduced and with 50 µM curcumin it was almost at the background level. When these arsenic treated cells were viewed under a fluorescence microscope, very intense fluorescence compared to the background was observed indicating generation of ROS; presence of curcumin appreciably reduced the fluorescence intensity indicating quenching action of curcumin. Photographs showing generation of ROS by arsenite and its quenching by curcumin are shown in Fig. 3 (b). Curcumin was also proved effective in inhibiting the lipid peroxidation caused by As III (Fig. 4). As represented in the figure the levels of TBARS were significantly increased from 100% in control lymphocytes (without any treatment) to 153% in cells treated with As 1000 µM. Administration of curcumin during this arsenite treatment significantly decreased the levels of TBARS by 84.6%, 72% and 52% in presence of 10, 25 and 50 µM respectively.

Bottom Line: Amelioration of such damages with natural compounds could be an effective strategy to combat arsenic toxicity.The expression of polymerase, a repair enzyme, was found to be highly elevated when arsenite induced damaged cells were allowed to repair in presence of curcumin.Results indicate that curcumin has significant role in confronting the deleterious effect caused by arsenic, which could be an economic mode of arsenic mitigation among rural population in West Bengal, India.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Carcinogenesis & Toxicology, Chittaranjan National Cancer Institute, 37 S.P. Mukherjee Road, Kolkata 700 026, India.

ABSTRACT
Chronic exposure of humans to high concentrations of arsenic in drinking water is associated with skin lesions, peripheral vascular disease, hypertension, blackfoot disease and a high risk of cancer. Arsenic induces single strand breaks, DNA-protein crosslinks and apurinic sites in DNA, which are prerequisites for induction of cancer. Amelioration of such damages with natural compounds could be an effective strategy to combat arsenic toxicity. Curcumin is the active ingredient of turmeric, a common household spice, which is a rich source of polyphenols and this compound has been extensively studied as a chemopreventive agent against many types of cancer. The present study investigates whether curcumin could counteract the DNA damage caused by arsenic as assessed by single cell gel electrophoresis (SCGE) using peripheral blood lymphocytes, from healthy donors. It was observed that DNA damage induced by arsenic could be efficiently reduced by curcumin and the effect was more pronounced when lymphocytes were pre-incubated with curcumin prior to arsenic insult. Arsenic caused DNA damage by generation of reactive oxygen species (ROS) and enhancement of lipid peroxidation levels. Curcumin counteracted the damage by quenching ROS, decreasing the level of lipid peroxidation and increasing the level of phase II detoxification enzymes like catalase, superoxide dismutase and glutathione peroxidase. Curcumin also enhanced the DNA repair activity against arsenic induced damage. The expression of polymerase, a repair enzyme, was found to be highly elevated when arsenite induced damaged cells were allowed to repair in presence of curcumin. Results indicate that curcumin has significant role in confronting the deleterious effect caused by arsenic, which could be an economic mode of arsenic mitigation among rural population in West Bengal, India.

No MeSH data available.


Related in: MedlinePlus