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Monocyte derived dendritic cells retain their functional capacity in patients following infection with hepatitis C virus.

Barnes E, Salio M, Cerundolo V, Francesco L, Pardoll D, Klenerman P, Cox A - J. Viral Hepat. (2008)

Bottom Line: DCs retained the same allostimulatory capacity before and following the establishment of persistent HCV infection.The surface phenotype and the amount of interleukin (IL)-10 and IL-12(p70) produced during DC maturation did not differ between HCV-infected individuals and healthy controls.Mature DCs from HCV-infected individuals performed comparably in an allogeneic MLR compared with healthy individuals.

View Article: PubMed Central - PubMed

Affiliation: Nuffield Department of Medicine, University of Oxford, Oxford, UK.

ABSTRACT
Studies assessing the function of monocyte derived dendritic cells (MD-DC) in individuals with hepatitis C virus (HCV) infection have shown conflicting results. Impaired MD-DC function in chronic HCV infection would have important implications both for understanding the pathogenesis of HCV infection and in the use of autologous MD-DC in vaccination strategies. We determined the allostimulatory capacity of MD-DC in the same patient before and after HCV infection. Next, the phenotype, cytokine production and allostimulatory function of immature and mature MD-DC in individuals with persistent HCV infection were compared directly with MD-DC from healthy individuals. Finally, we assessed the ability of MD-DC to prime autologous naïve peptide specific CD8+ T cells using HLA-A2 class-I tetramers. DCs retained the same allostimulatory capacity before and following the establishment of persistent HCV infection. The surface phenotype and the amount of interleukin (IL)-10 and IL-12(p70) produced during DC maturation did not differ between HCV-infected individuals and healthy controls. Mature DCs from HCV-infected individuals performed comparably in an allogeneic MLR compared with healthy individuals. Mature MD-DC from HCV-infected individuals stimulated the expansion of peptide specific naïve CD8+ T cells. MD-DC from HCV-infected and healthy individuals are phenotypically indistinguishable and perform comparably in functional assays.

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Related in: MedlinePlus

The phenotypic characteristics of immature and mature DCs in HCV infected and healthy individuals. The expression (mean fluorescence intensity–MFI) of HLA class I, HLA class II, CD 86 and CD83 on immature (bold line) and dsRNA matured (dotted line) MD-DCs from a representative HCV infected individual (HCV-2) and a healthy control individual is shown (HI-2) (A). The expression of these markers on MD-DCs is compared between healthy and HCV infected individuals on both immature (B, upper panel) and mature (B, lower panel) MD-DCs. The table inserts give the MFI ± standard error and P values for each marker.
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fig01: The phenotypic characteristics of immature and mature DCs in HCV infected and healthy individuals. The expression (mean fluorescence intensity–MFI) of HLA class I, HLA class II, CD 86 and CD83 on immature (bold line) and dsRNA matured (dotted line) MD-DCs from a representative HCV infected individual (HCV-2) and a healthy control individual is shown (HI-2) (A). The expression of these markers on MD-DCs is compared between healthy and HCV infected individuals on both immature (B, upper panel) and mature (B, lower panel) MD-DCs. The table inserts give the MFI ± standard error and P values for each marker.

Mentions: Monocyte derived dendritic cells were generated in parallel from five HCV-infected individuals and five healthy controls (Table 1). Immature MD-DC were matured with dsRNA. The expression of class-I (HLA-A, -B, -C), class-II (HLA-DR, -DQ and -DP), CD83 and CD86 before and following DC maturation was determined. As previously described [33], immature DCs expressed class-I, class-II and CD86 at intermediate levels, but not CD83. These markers were equally expressed in HCV-infected and healthy individuals (Figs. 1a,b, upper panel). Furthermore, dsRNA-induced DC maturation associated with the up-regulation of each of these markers in both HCV-infected and healthy individuals (Figs 1a,b, lower panel).


Monocyte derived dendritic cells retain their functional capacity in patients following infection with hepatitis C virus.

Barnes E, Salio M, Cerundolo V, Francesco L, Pardoll D, Klenerman P, Cox A - J. Viral Hepat. (2008)

The phenotypic characteristics of immature and mature DCs in HCV infected and healthy individuals. The expression (mean fluorescence intensity–MFI) of HLA class I, HLA class II, CD 86 and CD83 on immature (bold line) and dsRNA matured (dotted line) MD-DCs from a representative HCV infected individual (HCV-2) and a healthy control individual is shown (HI-2) (A). The expression of these markers on MD-DCs is compared between healthy and HCV infected individuals on both immature (B, upper panel) and mature (B, lower panel) MD-DCs. The table inserts give the MFI ± standard error and P values for each marker.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2268954&req=5

fig01: The phenotypic characteristics of immature and mature DCs in HCV infected and healthy individuals. The expression (mean fluorescence intensity–MFI) of HLA class I, HLA class II, CD 86 and CD83 on immature (bold line) and dsRNA matured (dotted line) MD-DCs from a representative HCV infected individual (HCV-2) and a healthy control individual is shown (HI-2) (A). The expression of these markers on MD-DCs is compared between healthy and HCV infected individuals on both immature (B, upper panel) and mature (B, lower panel) MD-DCs. The table inserts give the MFI ± standard error and P values for each marker.
Mentions: Monocyte derived dendritic cells were generated in parallel from five HCV-infected individuals and five healthy controls (Table 1). Immature MD-DC were matured with dsRNA. The expression of class-I (HLA-A, -B, -C), class-II (HLA-DR, -DQ and -DP), CD83 and CD86 before and following DC maturation was determined. As previously described [33], immature DCs expressed class-I, class-II and CD86 at intermediate levels, but not CD83. These markers were equally expressed in HCV-infected and healthy individuals (Figs. 1a,b, upper panel). Furthermore, dsRNA-induced DC maturation associated with the up-regulation of each of these markers in both HCV-infected and healthy individuals (Figs 1a,b, lower panel).

Bottom Line: DCs retained the same allostimulatory capacity before and following the establishment of persistent HCV infection.The surface phenotype and the amount of interleukin (IL)-10 and IL-12(p70) produced during DC maturation did not differ between HCV-infected individuals and healthy controls.Mature DCs from HCV-infected individuals performed comparably in an allogeneic MLR compared with healthy individuals.

View Article: PubMed Central - PubMed

Affiliation: Nuffield Department of Medicine, University of Oxford, Oxford, UK.

ABSTRACT
Studies assessing the function of monocyte derived dendritic cells (MD-DC) in individuals with hepatitis C virus (HCV) infection have shown conflicting results. Impaired MD-DC function in chronic HCV infection would have important implications both for understanding the pathogenesis of HCV infection and in the use of autologous MD-DC in vaccination strategies. We determined the allostimulatory capacity of MD-DC in the same patient before and after HCV infection. Next, the phenotype, cytokine production and allostimulatory function of immature and mature MD-DC in individuals with persistent HCV infection were compared directly with MD-DC from healthy individuals. Finally, we assessed the ability of MD-DC to prime autologous naïve peptide specific CD8+ T cells using HLA-A2 class-I tetramers. DCs retained the same allostimulatory capacity before and following the establishment of persistent HCV infection. The surface phenotype and the amount of interleukin (IL)-10 and IL-12(p70) produced during DC maturation did not differ between HCV-infected individuals and healthy controls. Mature DCs from HCV-infected individuals performed comparably in an allogeneic MLR compared with healthy individuals. Mature MD-DC from HCV-infected individuals stimulated the expansion of peptide specific naïve CD8+ T cells. MD-DC from HCV-infected and healthy individuals are phenotypically indistinguishable and perform comparably in functional assays.

Show MeSH
Related in: MedlinePlus