Limits...
Distribution of adenosine receptors in human sclera fibroblasts.

Cui D, Trier K, Chen X, Zeng J, Yang X, Hu J, Ge J - Mol. Vis. (2008)

Bottom Line: This was confirmed by western blot.ADORA2A was concentrated mainly in one side of the cytoplasm, and ADORA2B was found both in the nucleus and the cytoplasm.All four subtypes of ADOR were found in HSF and may play a role in scleral remodeling.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

ABSTRACT

Purpose: Systemic treatment with adenosine receptor antagonists has been reported to affect the biochemistry and ultrastructure of rabbit sclera. This study was conducted to determine whether adenosine receptors (ADORs) are present in human scleral fibroblasts (HSF).

Methods: Primary HSF were cultured in vitro and identified with anti-vimentin, anti-keratin, anti-desmin, and anti-S-100 antibodies. Confocal fluorescence microscopy was used to study the distribution of ADORs in the HSF cell lines and in the frozen human scleral sections. ADOR protein expression in HSF and human sclera was confirmed by western blot analysis of cell lysates.

Results: ADORs were expressed in both HSF and human sclera. This was confirmed by western blot. ADORA1 expression was concentrated in the nucleus. ADORA2A was concentrated mainly in one side of the cytoplasm, and ADORA2B was found both in the nucleus and the cytoplasm. ADORA3 was expressed weakly in the cytoplasm.

Conclusions: All four subtypes of ADOR were found in HSF and may play a role in scleral remodeling.

Show MeSH
Four subtypes of ADOR expressed in HSF and human sclera (HS). The marker ladder is from 10 kDa to 200 kDa. The molecular weight of ADORA1 is about 39 kDa, ADORA2A is 45 kDa, ADORA2B is about 52 kDa, and ADORA3 is nearly 36 kDa. ADORA1 and ADORA2B are strongly expressed in both the HSF and human sclera, but ADORA2A and ADORA3 are only weakly expressed in the human sclera.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2268861&req=5

f4: Four subtypes of ADOR expressed in HSF and human sclera (HS). The marker ladder is from 10 kDa to 200 kDa. The molecular weight of ADORA1 is about 39 kDa, ADORA2A is 45 kDa, ADORA2B is about 52 kDa, and ADORA3 is nearly 36 kDa. ADORA1 and ADORA2B are strongly expressed in both the HSF and human sclera, but ADORA2A and ADORA3 are only weakly expressed in the human sclera.

Mentions: As shown in Figure 4, four subtypes of ADOR protein were found in HSF and human sclera. The approximate molecular weight for ADORA1, ADORA2A, ADORA2B, and ADORA3 was 39 kDa, 45 kDa, 52 kDa, and 36 kDa, respectively. ADORA1 and ADORA2B were strongly expressed in both the HSF and human sclera. ADORA2A and ADORA3 were also expressed in both the HSF and human sclera but only weakly in the latter. In particular, ADORA3 was very weakly expressed in the human sclera.


Distribution of adenosine receptors in human sclera fibroblasts.

Cui D, Trier K, Chen X, Zeng J, Yang X, Hu J, Ge J - Mol. Vis. (2008)

Four subtypes of ADOR expressed in HSF and human sclera (HS). The marker ladder is from 10 kDa to 200 kDa. The molecular weight of ADORA1 is about 39 kDa, ADORA2A is 45 kDa, ADORA2B is about 52 kDa, and ADORA3 is nearly 36 kDa. ADORA1 and ADORA2B are strongly expressed in both the HSF and human sclera, but ADORA2A and ADORA3 are only weakly expressed in the human sclera.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2268861&req=5

f4: Four subtypes of ADOR expressed in HSF and human sclera (HS). The marker ladder is from 10 kDa to 200 kDa. The molecular weight of ADORA1 is about 39 kDa, ADORA2A is 45 kDa, ADORA2B is about 52 kDa, and ADORA3 is nearly 36 kDa. ADORA1 and ADORA2B are strongly expressed in both the HSF and human sclera, but ADORA2A and ADORA3 are only weakly expressed in the human sclera.
Mentions: As shown in Figure 4, four subtypes of ADOR protein were found in HSF and human sclera. The approximate molecular weight for ADORA1, ADORA2A, ADORA2B, and ADORA3 was 39 kDa, 45 kDa, 52 kDa, and 36 kDa, respectively. ADORA1 and ADORA2B were strongly expressed in both the HSF and human sclera. ADORA2A and ADORA3 were also expressed in both the HSF and human sclera but only weakly in the latter. In particular, ADORA3 was very weakly expressed in the human sclera.

Bottom Line: This was confirmed by western blot.ADORA2A was concentrated mainly in one side of the cytoplasm, and ADORA2B was found both in the nucleus and the cytoplasm.All four subtypes of ADOR were found in HSF and may play a role in scleral remodeling.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

ABSTRACT

Purpose: Systemic treatment with adenosine receptor antagonists has been reported to affect the biochemistry and ultrastructure of rabbit sclera. This study was conducted to determine whether adenosine receptors (ADORs) are present in human scleral fibroblasts (HSF).

Methods: Primary HSF were cultured in vitro and identified with anti-vimentin, anti-keratin, anti-desmin, and anti-S-100 antibodies. Confocal fluorescence microscopy was used to study the distribution of ADORs in the HSF cell lines and in the frozen human scleral sections. ADOR protein expression in HSF and human sclera was confirmed by western blot analysis of cell lysates.

Results: ADORs were expressed in both HSF and human sclera. This was confirmed by western blot. ADORA1 expression was concentrated in the nucleus. ADORA2A was concentrated mainly in one side of the cytoplasm, and ADORA2B was found both in the nucleus and the cytoplasm. ADORA3 was expressed weakly in the cytoplasm.

Conclusions: All four subtypes of ADOR were found in HSF and may play a role in scleral remodeling.

Show MeSH