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Comparative analysis of the tear protein profile in mycotic keratitis patients.

Ananthi S, Chitra T, Bini R, Prajna NV, Lalitha P, Dharmalingam K - Mol. Vis. (2008)

Bottom Line: Prolactin inducible protein and serum albumin precursor were upregulated in the infected samples.Cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin were downregulated in the infected samples.Tears can be used as a clinical source to study the proteomic responses in patients with fungal keratitis.

View Article: PubMed Central - PubMed

Affiliation: Dr.G.Venkataswamy Eye Research Institute, Aravind Medical Research Foundation, Aravind Eye Care System, Madurai, India.

ABSTRACT

Purpose: Mycotic keratitis is a major cause of corneal blindness in India. A proper understanding of the pathogenesis may help in refining the existing treatment. The purpose of this study is to examine the total tear protein profile of fungal keratitis patients, which may have a bearing on pathogenesis and disease progression.

Methods: Tear samples were collected from culture positive fungal keratitis patients. Tears from the uninfected fellow eye and from other healthy individuals served as controls. Two-dimensional electrophoresis (2DE) was used for the separation of fractionated tear proteins, and selected protein spots, which showed differential expressions, were identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Wherever needed, tag sequencing of peptide fragments using post source decay (PSD) was done to confirm the identification.

Results: The glutaredoxin-related protein was expressed only in the tears of fungal keratitis patients. Six other normal tear proteins were present in both samples but with varied expression levels. Prolactin inducible protein and serum albumin precursor were upregulated in the infected samples. Cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin were downregulated in the infected samples.

Conclusions: Tears can be used as a clinical source to study the proteomic responses in patients with fungal keratitis. The glutaredoxin-related protein is known to be produced by Aspergillus during oxidative stress conditions, and the presence of this protein in the tears of patients with mycotic keratitis indicates that this pathogen undergoes stress-related gene expression during infection.

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Related in: MedlinePlus

Magnified 2DE map of spots which were differentially expressed in the tears of mycotic keratitis patients. Among six normal tear proteins identified, four proteins (cystatin S precursor [1], cystatin SN precursor [5], cystatin [29], and human tear lipocalin [49]) were downregulated and two proteins (prolactin inducible protein [34] and serum albumin precursor [44]) were upregulated in fungal keratitis” in “Among six normal tear proteins identified, four proteins (cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin) were downregulated and two proteins (prolactin inducible protein and serum albumin precursor) were upregulated in fungal keratitis.
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f2: Magnified 2DE map of spots which were differentially expressed in the tears of mycotic keratitis patients. Among six normal tear proteins identified, four proteins (cystatin S precursor [1], cystatin SN precursor [5], cystatin [29], and human tear lipocalin [49]) were downregulated and two proteins (prolactin inducible protein [34] and serum albumin precursor [44]) were upregulated in fungal keratitis” in “Among six normal tear proteins identified, four proteins (cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin) were downregulated and two proteins (prolactin inducible protein and serum albumin precursor) were upregulated in fungal keratitis.

Mentions: The glutaredoxin-related protein was found to be expressed only in the infected tear samples and was absent in the control tear samples. The expression of this protein was higher in the tear samples obtained from Aspergillus keratitis than in the tear samples obtained from Fusarium keratitis. In addition, six other normal tear proteins were also shown to be differentially regulated. Among the six normal tear proteins, prolactin inducible protein and serum albumin precursor were upregulated while cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin were downregulated in the infected tear when compared to the control tear sample (Figure 2).


Comparative analysis of the tear protein profile in mycotic keratitis patients.

Ananthi S, Chitra T, Bini R, Prajna NV, Lalitha P, Dharmalingam K - Mol. Vis. (2008)

Magnified 2DE map of spots which were differentially expressed in the tears of mycotic keratitis patients. Among six normal tear proteins identified, four proteins (cystatin S precursor [1], cystatin SN precursor [5], cystatin [29], and human tear lipocalin [49]) were downregulated and two proteins (prolactin inducible protein [34] and serum albumin precursor [44]) were upregulated in fungal keratitis” in “Among six normal tear proteins identified, four proteins (cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin) were downregulated and two proteins (prolactin inducible protein and serum albumin precursor) were upregulated in fungal keratitis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2268856&req=5

f2: Magnified 2DE map of spots which were differentially expressed in the tears of mycotic keratitis patients. Among six normal tear proteins identified, four proteins (cystatin S precursor [1], cystatin SN precursor [5], cystatin [29], and human tear lipocalin [49]) were downregulated and two proteins (prolactin inducible protein [34] and serum albumin precursor [44]) were upregulated in fungal keratitis” in “Among six normal tear proteins identified, four proteins (cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin) were downregulated and two proteins (prolactin inducible protein and serum albumin precursor) were upregulated in fungal keratitis.
Mentions: The glutaredoxin-related protein was found to be expressed only in the infected tear samples and was absent in the control tear samples. The expression of this protein was higher in the tear samples obtained from Aspergillus keratitis than in the tear samples obtained from Fusarium keratitis. In addition, six other normal tear proteins were also shown to be differentially regulated. Among the six normal tear proteins, prolactin inducible protein and serum albumin precursor were upregulated while cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin were downregulated in the infected tear when compared to the control tear sample (Figure 2).

Bottom Line: Prolactin inducible protein and serum albumin precursor were upregulated in the infected samples.Cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin were downregulated in the infected samples.Tears can be used as a clinical source to study the proteomic responses in patients with fungal keratitis.

View Article: PubMed Central - PubMed

Affiliation: Dr.G.Venkataswamy Eye Research Institute, Aravind Medical Research Foundation, Aravind Eye Care System, Madurai, India.

ABSTRACT

Purpose: Mycotic keratitis is a major cause of corneal blindness in India. A proper understanding of the pathogenesis may help in refining the existing treatment. The purpose of this study is to examine the total tear protein profile of fungal keratitis patients, which may have a bearing on pathogenesis and disease progression.

Methods: Tear samples were collected from culture positive fungal keratitis patients. Tears from the uninfected fellow eye and from other healthy individuals served as controls. Two-dimensional electrophoresis (2DE) was used for the separation of fractionated tear proteins, and selected protein spots, which showed differential expressions, were identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Wherever needed, tag sequencing of peptide fragments using post source decay (PSD) was done to confirm the identification.

Results: The glutaredoxin-related protein was expressed only in the tears of fungal keratitis patients. Six other normal tear proteins were present in both samples but with varied expression levels. Prolactin inducible protein and serum albumin precursor were upregulated in the infected samples. Cystatin S precursor, cystatin SN precursor, cystatin, and human tear lipocalin were downregulated in the infected samples.

Conclusions: Tears can be used as a clinical source to study the proteomic responses in patients with fungal keratitis. The glutaredoxin-related protein is known to be produced by Aspergillus during oxidative stress conditions, and the presence of this protein in the tears of patients with mycotic keratitis indicates that this pathogen undergoes stress-related gene expression during infection.

Show MeSH
Related in: MedlinePlus