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Ultrasonic vocalizations induced by sex and amphetamine in M2, M4, M5 muscarinic and D2 dopamine receptor knockout mice.

Wang H, Liang S, Burgdorf J, Wess J, Yeomans J - PLoS ONE (2008)

Bottom Line: In M4 KOs, no significant differences were observed.These studies suggest that M2 and M5 muscarinic receptors are needed for male USV production during male-female interactions, likely via their roles in dopamine activation.These findings are important for the understanding of the neural substrates for positive affect.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, Center for Biological Timing and Cognition, University of Toronto, Toronto, Canada. haoranw@psych.toronto.edu

ABSTRACT
Adult mice communicate by emitting ultrasonic vocalizations (USVs) during the appetitive phases of sexual behavior. However, little is known about the genes important in controlling call production. Here, we study the induction and regulation of USVs in muscarinic and dopaminergic receptor knockout (KO) mice as well as wild-type controls during sexual behavior. Female mouse urine, but not female rat or human urine, induced USVs in male mice, whereas male urine did not induce USVs in females. Direct contact of males with females is required for eliciting high level of USVs in males. USVs (25 to120 kHz) were emitted only by males, suggesting positive state; however human-audible squeaks were produced only by females, implying negative state during male-female pairing. USVs were divided into flat and frequency-modulated calls. Male USVs often changed from continuous to broken frequency-modulated calls after initiation of mounting. In M2 KO mice, USVs were lost in about 70-80% of the mice, correlating with a loss of sexual interaction. In M5 KO mice, mean USVs were reduced by almost 80% even though sexual interaction was vigorous. In D2 KOs, the duration of USVs was extended by 20%. In M4 KOs, no significant differences were observed. Amphetamine dose-dependently induced USVs in wild-type males (most at 0.5 mg/kg i.p.), but did not elicit USVs in M5 KO or female mice. These studies suggest that M2 and M5 muscarinic receptors are needed for male USV production during male-female interactions, likely via their roles in dopamine activation. These findings are important for the understanding of the neural substrates for positive affect.

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Coincidence of mounting behaviour and production of USVs by males and squeaks by females in Exp. 4.Coincidence of mounting behaviour and production of USVs and squeaks were analyzed by comparing the sonograms of mouse calls before, during, and after mounting behavior with simultaneously recorded videos as described in Materials and Methods. (A) Representative USVs emitted by male mice before mounting. (B) Male USVs shifted from “sine” to “step-like” USVs after initiation of male- mounting-female. (C) Coincidence of frequency-modulated “step-like” USVs emitted by male mice and squeaks produced by female mice during mounting/intromission. (D) No call was detected within about 10 sec after the end of mounting (full data not shown).
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pone-0001893-g005: Coincidence of mounting behaviour and production of USVs by males and squeaks by females in Exp. 4.Coincidence of mounting behaviour and production of USVs and squeaks were analyzed by comparing the sonograms of mouse calls before, during, and after mounting behavior with simultaneously recorded videos as described in Materials and Methods. (A) Representative USVs emitted by male mice before mounting. (B) Male USVs shifted from “sine” to “step-like” USVs after initiation of male- mounting-female. (C) Coincidence of frequency-modulated “step-like” USVs emitted by male mice and squeaks produced by female mice during mounting/intromission. (D) No call was detected within about 10 sec after the end of mounting (full data not shown).

Mentions: As described above, males called in USVs and females called in squeaks during male-female interaction. To study this more carefully, we investigated the coincidence of sexual behaviour (e.g., mounting) and production of USVs and squeaks. Before mounting, males usually called in either flat or continuous frequency-modulated USVs (Figure 5A). However, when males mounted females, males shifted to frequency-modulated broken “step-like” USVs with 70-kHz and 40/80 harmonic frequencies until the end of mounting (Figure 5B and C). Meanwhile, females called in squeaks which often overlapped with USVs (Figure 5C). These calls lasted as long as 20 sec. At the end of mounting, there usually followed an approximately 10 sec (10.66±2.58 sec, mean±SD) period of call-absence (Figure 5D). These results indicate a dynamic change of male USVs and an occurrence of female squeaks during mounting behavior.


Ultrasonic vocalizations induced by sex and amphetamine in M2, M4, M5 muscarinic and D2 dopamine receptor knockout mice.

Wang H, Liang S, Burgdorf J, Wess J, Yeomans J - PLoS ONE (2008)

Coincidence of mounting behaviour and production of USVs by males and squeaks by females in Exp. 4.Coincidence of mounting behaviour and production of USVs and squeaks were analyzed by comparing the sonograms of mouse calls before, during, and after mounting behavior with simultaneously recorded videos as described in Materials and Methods. (A) Representative USVs emitted by male mice before mounting. (B) Male USVs shifted from “sine” to “step-like” USVs after initiation of male- mounting-female. (C) Coincidence of frequency-modulated “step-like” USVs emitted by male mice and squeaks produced by female mice during mounting/intromission. (D) No call was detected within about 10 sec after the end of mounting (full data not shown).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2268741&req=5

pone-0001893-g005: Coincidence of mounting behaviour and production of USVs by males and squeaks by females in Exp. 4.Coincidence of mounting behaviour and production of USVs and squeaks were analyzed by comparing the sonograms of mouse calls before, during, and after mounting behavior with simultaneously recorded videos as described in Materials and Methods. (A) Representative USVs emitted by male mice before mounting. (B) Male USVs shifted from “sine” to “step-like” USVs after initiation of male- mounting-female. (C) Coincidence of frequency-modulated “step-like” USVs emitted by male mice and squeaks produced by female mice during mounting/intromission. (D) No call was detected within about 10 sec after the end of mounting (full data not shown).
Mentions: As described above, males called in USVs and females called in squeaks during male-female interaction. To study this more carefully, we investigated the coincidence of sexual behaviour (e.g., mounting) and production of USVs and squeaks. Before mounting, males usually called in either flat or continuous frequency-modulated USVs (Figure 5A). However, when males mounted females, males shifted to frequency-modulated broken “step-like” USVs with 70-kHz and 40/80 harmonic frequencies until the end of mounting (Figure 5B and C). Meanwhile, females called in squeaks which often overlapped with USVs (Figure 5C). These calls lasted as long as 20 sec. At the end of mounting, there usually followed an approximately 10 sec (10.66±2.58 sec, mean±SD) period of call-absence (Figure 5D). These results indicate a dynamic change of male USVs and an occurrence of female squeaks during mounting behavior.

Bottom Line: In M4 KOs, no significant differences were observed.These studies suggest that M2 and M5 muscarinic receptors are needed for male USV production during male-female interactions, likely via their roles in dopamine activation.These findings are important for the understanding of the neural substrates for positive affect.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, Center for Biological Timing and Cognition, University of Toronto, Toronto, Canada. haoranw@psych.toronto.edu

ABSTRACT
Adult mice communicate by emitting ultrasonic vocalizations (USVs) during the appetitive phases of sexual behavior. However, little is known about the genes important in controlling call production. Here, we study the induction and regulation of USVs in muscarinic and dopaminergic receptor knockout (KO) mice as well as wild-type controls during sexual behavior. Female mouse urine, but not female rat or human urine, induced USVs in male mice, whereas male urine did not induce USVs in females. Direct contact of males with females is required for eliciting high level of USVs in males. USVs (25 to120 kHz) were emitted only by males, suggesting positive state; however human-audible squeaks were produced only by females, implying negative state during male-female pairing. USVs were divided into flat and frequency-modulated calls. Male USVs often changed from continuous to broken frequency-modulated calls after initiation of mounting. In M2 KO mice, USVs were lost in about 70-80% of the mice, correlating with a loss of sexual interaction. In M5 KO mice, mean USVs were reduced by almost 80% even though sexual interaction was vigorous. In D2 KOs, the duration of USVs was extended by 20%. In M4 KOs, no significant differences were observed. Amphetamine dose-dependently induced USVs in wild-type males (most at 0.5 mg/kg i.p.), but did not elicit USVs in M5 KO or female mice. These studies suggest that M2 and M5 muscarinic receptors are needed for male USV production during male-female interactions, likely via their roles in dopamine activation. These findings are important for the understanding of the neural substrates for positive affect.

Show MeSH
Related in: MedlinePlus