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Ultrasonic vocalizations induced by sex and amphetamine in M2, M4, M5 muscarinic and D2 dopamine receptor knockout mice.

Wang H, Liang S, Burgdorf J, Wess J, Yeomans J - PLoS ONE (2008)

Bottom Line: In M4 KOs, no significant differences were observed.These studies suggest that M2 and M5 muscarinic receptors are needed for male USV production during male-female interactions, likely via their roles in dopamine activation.These findings are important for the understanding of the neural substrates for positive affect.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, Center for Biological Timing and Cognition, University of Toronto, Toronto, Canada. haoranw@psych.toronto.edu

ABSTRACT
Adult mice communicate by emitting ultrasonic vocalizations (USVs) during the appetitive phases of sexual behavior. However, little is known about the genes important in controlling call production. Here, we study the induction and regulation of USVs in muscarinic and dopaminergic receptor knockout (KO) mice as well as wild-type controls during sexual behavior. Female mouse urine, but not female rat or human urine, induced USVs in male mice, whereas male urine did not induce USVs in females. Direct contact of males with females is required for eliciting high level of USVs in males. USVs (25 to120 kHz) were emitted only by males, suggesting positive state; however human-audible squeaks were produced only by females, implying negative state during male-female pairing. USVs were divided into flat and frequency-modulated calls. Male USVs often changed from continuous to broken frequency-modulated calls after initiation of mounting. In M2 KO mice, USVs were lost in about 70-80% of the mice, correlating with a loss of sexual interaction. In M5 KO mice, mean USVs were reduced by almost 80% even though sexual interaction was vigorous. In D2 KOs, the duration of USVs was extended by 20%. In M4 KOs, no significant differences were observed. Amphetamine dose-dependently induced USVs in wild-type males (most at 0.5 mg/kg i.p.), but did not elicit USVs in M5 KO or female mice. These studies suggest that M2 and M5 muscarinic receptors are needed for male USV production during male-female interactions, likely via their roles in dopamine activation. These findings are important for the understanding of the neural substrates for positive affect.

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Males and females call differently during male-female interaction in Exp. 2, 4 and 5.(A) Representative sonograms of mouse ultrasonic vocalizations produced during male-female interaction. 4A1: flat USV, 4A2: frequency-modulated sine-wave-like USV and 4A3: broken USV, and 4A4: breached-harmonic USV. 4A5: human audible dense-layered squeaks. (B) No USVs were detected when male were silenced by simple mouth taping; but when females were silenced, many USVs were detected, suggesting that USVs recorded during male-female interaction were emitted from the male. (C) After aggressive attacks from male mice, females produced a few squeaks, observed on sonograms as harmonic dense-layered calls. (D) When reunited with another female after 4 hour's separation as described before [73], female mice emitted frequency-modulated USVs as those produced by male mice during male-female interaction. Three representative broken USVs were shown.
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pone-0001893-g003: Males and females call differently during male-female interaction in Exp. 2, 4 and 5.(A) Representative sonograms of mouse ultrasonic vocalizations produced during male-female interaction. 4A1: flat USV, 4A2: frequency-modulated sine-wave-like USV and 4A3: broken USV, and 4A4: breached-harmonic USV. 4A5: human audible dense-layered squeaks. (B) No USVs were detected when male were silenced by simple mouth taping; but when females were silenced, many USVs were detected, suggesting that USVs recorded during male-female interaction were emitted from the male. (C) After aggressive attacks from male mice, females produced a few squeaks, observed on sonograms as harmonic dense-layered calls. (D) When reunited with another female after 4 hour's separation as described before [73], female mice emitted frequency-modulated USVs as those produced by male mice during male-female interaction. Three representative broken USVs were shown.

Mentions: Considering the influence of different genetic backgrounds of the mice and other inducing factors on the patterns of calls [1], [13], [14], [47], 3 representative sonograms of mouse (CD1x129) USVs obtained during male-female interaction are shown in Figure 3A. These are flat USVs (bandwidth ≤5-kHz) (Figure 3A1), frequency-modulated sine-wave-like USVs (Figure 3A2, bandwidth >5-kHz), and broken USVs (Figure 3A3), respectively. A representative squeak is also shown (Figure 3A4).


Ultrasonic vocalizations induced by sex and amphetamine in M2, M4, M5 muscarinic and D2 dopamine receptor knockout mice.

Wang H, Liang S, Burgdorf J, Wess J, Yeomans J - PLoS ONE (2008)

Males and females call differently during male-female interaction in Exp. 2, 4 and 5.(A) Representative sonograms of mouse ultrasonic vocalizations produced during male-female interaction. 4A1: flat USV, 4A2: frequency-modulated sine-wave-like USV and 4A3: broken USV, and 4A4: breached-harmonic USV. 4A5: human audible dense-layered squeaks. (B) No USVs were detected when male were silenced by simple mouth taping; but when females were silenced, many USVs were detected, suggesting that USVs recorded during male-female interaction were emitted from the male. (C) After aggressive attacks from male mice, females produced a few squeaks, observed on sonograms as harmonic dense-layered calls. (D) When reunited with another female after 4 hour's separation as described before [73], female mice emitted frequency-modulated USVs as those produced by male mice during male-female interaction. Three representative broken USVs were shown.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2268741&req=5

pone-0001893-g003: Males and females call differently during male-female interaction in Exp. 2, 4 and 5.(A) Representative sonograms of mouse ultrasonic vocalizations produced during male-female interaction. 4A1: flat USV, 4A2: frequency-modulated sine-wave-like USV and 4A3: broken USV, and 4A4: breached-harmonic USV. 4A5: human audible dense-layered squeaks. (B) No USVs were detected when male were silenced by simple mouth taping; but when females were silenced, many USVs were detected, suggesting that USVs recorded during male-female interaction were emitted from the male. (C) After aggressive attacks from male mice, females produced a few squeaks, observed on sonograms as harmonic dense-layered calls. (D) When reunited with another female after 4 hour's separation as described before [73], female mice emitted frequency-modulated USVs as those produced by male mice during male-female interaction. Three representative broken USVs were shown.
Mentions: Considering the influence of different genetic backgrounds of the mice and other inducing factors on the patterns of calls [1], [13], [14], [47], 3 representative sonograms of mouse (CD1x129) USVs obtained during male-female interaction are shown in Figure 3A. These are flat USVs (bandwidth ≤5-kHz) (Figure 3A1), frequency-modulated sine-wave-like USVs (Figure 3A2, bandwidth >5-kHz), and broken USVs (Figure 3A3), respectively. A representative squeak is also shown (Figure 3A4).

Bottom Line: In M4 KOs, no significant differences were observed.These studies suggest that M2 and M5 muscarinic receptors are needed for male USV production during male-female interactions, likely via their roles in dopamine activation.These findings are important for the understanding of the neural substrates for positive affect.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, Center for Biological Timing and Cognition, University of Toronto, Toronto, Canada. haoranw@psych.toronto.edu

ABSTRACT
Adult mice communicate by emitting ultrasonic vocalizations (USVs) during the appetitive phases of sexual behavior. However, little is known about the genes important in controlling call production. Here, we study the induction and regulation of USVs in muscarinic and dopaminergic receptor knockout (KO) mice as well as wild-type controls during sexual behavior. Female mouse urine, but not female rat or human urine, induced USVs in male mice, whereas male urine did not induce USVs in females. Direct contact of males with females is required for eliciting high level of USVs in males. USVs (25 to120 kHz) were emitted only by males, suggesting positive state; however human-audible squeaks were produced only by females, implying negative state during male-female pairing. USVs were divided into flat and frequency-modulated calls. Male USVs often changed from continuous to broken frequency-modulated calls after initiation of mounting. In M2 KO mice, USVs were lost in about 70-80% of the mice, correlating with a loss of sexual interaction. In M5 KO mice, mean USVs were reduced by almost 80% even though sexual interaction was vigorous. In D2 KOs, the duration of USVs was extended by 20%. In M4 KOs, no significant differences were observed. Amphetamine dose-dependently induced USVs in wild-type males (most at 0.5 mg/kg i.p.), but did not elicit USVs in M5 KO or female mice. These studies suggest that M2 and M5 muscarinic receptors are needed for male USV production during male-female interactions, likely via their roles in dopamine activation. These findings are important for the understanding of the neural substrates for positive affect.

Show MeSH
Related in: MedlinePlus