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Circulating beta-endorphin, adrenocorticotrophic hormone and cortisol levels of stallions before and after short road transport: stress effect of different distances.

Fazio E, Medica P, Aronica V, Grasso L, Ferlazzo A - Acta Vet. Scand. (2008)

Bottom Line: An increase in circulating beta-endorphin levels after transport of 100 km (P < 0.01), compared to basal values was observed.Circulating ACTH levels showed significant increases after transport of 100 km (P < 0.001) and 200 km (P < 0.001).The results obtained for short term transportation of stallions showed a very strong reaction of the adrenocortical system.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Morphology, Biochemistry, Physiology and Animal Production, Unit of Veterinary Physiology, Faculty of Veterinary Medicine, University of Messina, 98168 Messina, Italy. esterina.fazio@tin.it

ABSTRACT

Background: Since transport evokes physiological adjustments that include endocrine responses, the objective of this study was to examine the responses of circulating beta-endorphin, adrenocorticotrophic hormone (ACTH) and cortisol levels to transport stress in stallions.

Methods: Forty-two healthy Thoroughbred and crossbred stallions were studied before and after road transport over distances of 100, 200 and 300 km. Blood samples were collected from the jugular vein: first in a single box immediately before loading (pre-samples), then immediately after transport and unloading on arrival at the breeding stations (post-samples).

Results: An increase in circulating beta-endorphin levels after transport of 100 km (P < 0.01), compared to basal values was observed. Circulating ACTH levels showed significant increases after transport of 100 km (P < 0.001) and 200 km (P < 0.001). Circulating cortisol levels showed significant increases after road transport over distances of 100, 200 and 300 km (P < 0.001). An effect of transport on beta-endorphin, ACTH and cortisol variations was therefore evident for the different distances studied. No significant differences (P > 0.05) between horses of different ages and different breeds were observed for beta-endorphin, ACTH and cortisol levels.

Conclusion: The results obtained for short term transportation of stallions showed a very strong reaction of the adrenocortical system. The lack of response of beta-endorphin after transport of 200-300 km and of ACTH after transport of 300 km seems to suggest a soothing effect of negative feedback of ACTH and cortisol levels.

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Circulating β-endorphin concentrations (mean ± SD) of stallions before and after short road transport of different distances. Label on X-axis: Groups, Distances (km), number of subjects. Asterisk indicates significant (*P < 0.001) differences vs before. Symbol indicates significant (°P < 0.01) differences vs Group I.
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Figure 1: Circulating β-endorphin concentrations (mean ± SD) of stallions before and after short road transport of different distances. Label on X-axis: Groups, Distances (km), number of subjects. Asterisk indicates significant (*P < 0.001) differences vs before. Symbol indicates significant (°P < 0.01) differences vs Group I.

Mentions: Circulating β-endorphin levels showed an increase (Figure 1) after road transport in Group I (100 Km: P < 0.01), compared to basal values. Thus, an effect of transport was shown for a distance of 100 km (P < 0.001).


Circulating beta-endorphin, adrenocorticotrophic hormone and cortisol levels of stallions before and after short road transport: stress effect of different distances.

Fazio E, Medica P, Aronica V, Grasso L, Ferlazzo A - Acta Vet. Scand. (2008)

Circulating β-endorphin concentrations (mean ± SD) of stallions before and after short road transport of different distances. Label on X-axis: Groups, Distances (km), number of subjects. Asterisk indicates significant (*P < 0.001) differences vs before. Symbol indicates significant (°P < 0.01) differences vs Group I.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2268694&req=5

Figure 1: Circulating β-endorphin concentrations (mean ± SD) of stallions before and after short road transport of different distances. Label on X-axis: Groups, Distances (km), number of subjects. Asterisk indicates significant (*P < 0.001) differences vs before. Symbol indicates significant (°P < 0.01) differences vs Group I.
Mentions: Circulating β-endorphin levels showed an increase (Figure 1) after road transport in Group I (100 Km: P < 0.01), compared to basal values. Thus, an effect of transport was shown for a distance of 100 km (P < 0.001).

Bottom Line: An increase in circulating beta-endorphin levels after transport of 100 km (P < 0.01), compared to basal values was observed.Circulating ACTH levels showed significant increases after transport of 100 km (P < 0.001) and 200 km (P < 0.001).The results obtained for short term transportation of stallions showed a very strong reaction of the adrenocortical system.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Morphology, Biochemistry, Physiology and Animal Production, Unit of Veterinary Physiology, Faculty of Veterinary Medicine, University of Messina, 98168 Messina, Italy. esterina.fazio@tin.it

ABSTRACT

Background: Since transport evokes physiological adjustments that include endocrine responses, the objective of this study was to examine the responses of circulating beta-endorphin, adrenocorticotrophic hormone (ACTH) and cortisol levels to transport stress in stallions.

Methods: Forty-two healthy Thoroughbred and crossbred stallions were studied before and after road transport over distances of 100, 200 and 300 km. Blood samples were collected from the jugular vein: first in a single box immediately before loading (pre-samples), then immediately after transport and unloading on arrival at the breeding stations (post-samples).

Results: An increase in circulating beta-endorphin levels after transport of 100 km (P < 0.01), compared to basal values was observed. Circulating ACTH levels showed significant increases after transport of 100 km (P < 0.001) and 200 km (P < 0.001). Circulating cortisol levels showed significant increases after road transport over distances of 100, 200 and 300 km (P < 0.001). An effect of transport on beta-endorphin, ACTH and cortisol variations was therefore evident for the different distances studied. No significant differences (P > 0.05) between horses of different ages and different breeds were observed for beta-endorphin, ACTH and cortisol levels.

Conclusion: The results obtained for short term transportation of stallions showed a very strong reaction of the adrenocortical system. The lack of response of beta-endorphin after transport of 200-300 km and of ACTH after transport of 300 km seems to suggest a soothing effect of negative feedback of ACTH and cortisol levels.

Show MeSH
Related in: MedlinePlus