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Identification of a subunit of NADH-dehydrogenase as a p49/STRAP-binding protein.

Zhang X, Azhar G, Helms S, Zhong Y, Wei JY - BMC Cell Biol. (2008)

Bottom Line: Overexpression of p49/STRAP altered the intracellular level of NAD, and reduced the NAD/NADH ratio.Overexpression of p49/STRAP also induced the deacetylation of serum response factor.These data suggest that p49/STRAP plays a role in the regulation of intracellular processes such as cardiac cellular metabolism, gene expression, and possibly aging.

View Article: PubMed Central - HTML - PubMed

Affiliation: From the Donald W. Reynolds Department of Geriatrics, The University of Arkansas for Medical Sciences and Geriatric Research, Education, and Clinical Center, Little Rock, AR, USA. zhangxiaomin@uams.edu

ABSTRACT

Background: The p49/STRAP (or SRFBP1) protein was recently identified in our laboratory as a cofactor of serum response factor that contributes to the regulation of SRF target genes in the heart.

Results: In the present study, we report that NDUFAB1, a nuclear encoded subunit of NADH dehydrogenase, represented the majority of the cDNA clones that interacted with p49/STRAP in multiple screenings using the yeast two-hybrid system. The p49/STRAP and NDUFAB1 proteins interacted and co-localized with each other in the cell. The p49/STRAP protein contains four classic nuclear localization sequence motifs, and it was observed to be present predominantly in the nucleus. Overexpression of p49/STRAP altered the intracellular level of NAD, and reduced the NAD/NADH ratio. Overexpression of p49/STRAP also induced the deacetylation of serum response factor.

Conclusion: These data suggest that p49/STRAP plays a role in the regulation of intracellular processes such as cardiac cellular metabolism, gene expression, and possibly aging.

Show MeSH
Intracellular localization of p49 and NDUFAB1. Fluorescent images of H9C2 cells transfected with NDUFAB1-RFP expression plasmids (a, top left) and RFP control (b, top right), demonstrate both nuclear and cytoplasmic distribution. The p49-GFP fusion protein appears to be primarily localized within the nucleus, especially the nucleoli (c, bottom left). The GFP-only control protein is distributed evenly throughout the cells (d, bottom right). GFP = green fluorescent protein; RFP red fluorescent protein.
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Figure 3: Intracellular localization of p49 and NDUFAB1. Fluorescent images of H9C2 cells transfected with NDUFAB1-RFP expression plasmids (a, top left) and RFP control (b, top right), demonstrate both nuclear and cytoplasmic distribution. The p49-GFP fusion protein appears to be primarily localized within the nucleus, especially the nucleoli (c, bottom left). The GFP-only control protein is distributed evenly throughout the cells (d, bottom right). GFP = green fluorescent protein; RFP red fluorescent protein.

Mentions: NDUFAB1, which is a nuclear-encoded protein, is a subunit of the mitochondrial respiratory Complex I. To visualize the localization of NDUFAB1 in living cells, a red fluorescent protein (RFP), mCherry, was linked in-frame with NDUFAB1 and expressed in the H9C2 cells. As shown in figure 3a, the RFP-NDUFAB1 was observed to be present in both the cytoplasm and nucleus.


Identification of a subunit of NADH-dehydrogenase as a p49/STRAP-binding protein.

Zhang X, Azhar G, Helms S, Zhong Y, Wei JY - BMC Cell Biol. (2008)

Intracellular localization of p49 and NDUFAB1. Fluorescent images of H9C2 cells transfected with NDUFAB1-RFP expression plasmids (a, top left) and RFP control (b, top right), demonstrate both nuclear and cytoplasmic distribution. The p49-GFP fusion protein appears to be primarily localized within the nucleus, especially the nucleoli (c, bottom left). The GFP-only control protein is distributed evenly throughout the cells (d, bottom right). GFP = green fluorescent protein; RFP red fluorescent protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2268686&req=5

Figure 3: Intracellular localization of p49 and NDUFAB1. Fluorescent images of H9C2 cells transfected with NDUFAB1-RFP expression plasmids (a, top left) and RFP control (b, top right), demonstrate both nuclear and cytoplasmic distribution. The p49-GFP fusion protein appears to be primarily localized within the nucleus, especially the nucleoli (c, bottom left). The GFP-only control protein is distributed evenly throughout the cells (d, bottom right). GFP = green fluorescent protein; RFP red fluorescent protein.
Mentions: NDUFAB1, which is a nuclear-encoded protein, is a subunit of the mitochondrial respiratory Complex I. To visualize the localization of NDUFAB1 in living cells, a red fluorescent protein (RFP), mCherry, was linked in-frame with NDUFAB1 and expressed in the H9C2 cells. As shown in figure 3a, the RFP-NDUFAB1 was observed to be present in both the cytoplasm and nucleus.

Bottom Line: Overexpression of p49/STRAP altered the intracellular level of NAD, and reduced the NAD/NADH ratio.Overexpression of p49/STRAP also induced the deacetylation of serum response factor.These data suggest that p49/STRAP plays a role in the regulation of intracellular processes such as cardiac cellular metabolism, gene expression, and possibly aging.

View Article: PubMed Central - HTML - PubMed

Affiliation: From the Donald W. Reynolds Department of Geriatrics, The University of Arkansas for Medical Sciences and Geriatric Research, Education, and Clinical Center, Little Rock, AR, USA. zhangxiaomin@uams.edu

ABSTRACT

Background: The p49/STRAP (or SRFBP1) protein was recently identified in our laboratory as a cofactor of serum response factor that contributes to the regulation of SRF target genes in the heart.

Results: In the present study, we report that NDUFAB1, a nuclear encoded subunit of NADH dehydrogenase, represented the majority of the cDNA clones that interacted with p49/STRAP in multiple screenings using the yeast two-hybrid system. The p49/STRAP and NDUFAB1 proteins interacted and co-localized with each other in the cell. The p49/STRAP protein contains four classic nuclear localization sequence motifs, and it was observed to be present predominantly in the nucleus. Overexpression of p49/STRAP altered the intracellular level of NAD, and reduced the NAD/NADH ratio. Overexpression of p49/STRAP also induced the deacetylation of serum response factor.

Conclusion: These data suggest that p49/STRAP plays a role in the regulation of intracellular processes such as cardiac cellular metabolism, gene expression, and possibly aging.

Show MeSH