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A surrogate method for comparison analysis of salivary concentrations of Xylitol-containing products.

Riedy CA, Milgrom P, Ly KA, Rothen M, Mueller G, Hagstrom MK, Tolentino E, Zhou L, Roberts MC - BMC Oral Health (2008)

Bottom Line: Study 1 had its highest mean peaks at the 4 min sampling point while Study 2 had its highest mean peaks between 13 to 16 minutes.Salivary xylitol levels returned to baseline at about 18 minutes for all forms tested.The comparison method demonstrated high reliability and validity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Northwest/Alaska Center to Reduce Oral Health Disparities and Department of Dental Public Health Sciences, School of Dentistry, University of Washington, Seattle, WA, USA. cariedy@u.washington.edu

ABSTRACT

Background: Xylitol chewing gum has been shown to reduce Streptococcus mutans levels and decay. Two studies examined the presence and time course of salivary xylitol concentrations delivered via xylitol-containing pellet gum and compared them to other xylitol-containing products.

Methods: A within-subjects design was used for both studies. Study 1, adults (N = 15) received three xylitol-containing products (pellet gum (2.6 g), gummy bears (2.6 g), and commercially available stick gum (Koolerz, 3.0 g)); Study 2, a second group of adults (N = 15) received three xylitol-containing products (pellet gum, gummy bears, and a 33% xylitol syrup (2.67 g). For both studies subjects consumed one xylitol product per visit with a 7-day washout between each product. A standardized protocol was followed for each product visit. Product order was randomly determined at the initial visit. Saliva samples (0.5 mL to 1.0 mL) were collected at baseline and up to 10 time points (approximately 16 min in length) after product consumption initiated. Concentration of xylitol in saliva samples was analyzed using high-performance liquid chromatography. Area under the curve (AUC) for determining the average xylitol concentration in saliva over the total sampling period was calculated for each product.

Results: In both studies all three xylitol products (Study 1: pellet gum, gummy bears, and stick gum; Study 2: pellet gum, gummy bears, and syrup) had similar time curves with two xylitol concentration peaks during the sampling period. Study 1 had its highest mean peaks at the 4 min sampling point while Study 2 had its highest mean peaks between 13 to 16 minutes. Salivary xylitol levels returned to baseline at about 18 minutes for all forms tested. Additionally, for both studies the total AUC for the xylitol products were similar compared to the pellet gum (Study 1: pellet gum - 51.3 microg x min/mL, gummy bears - 59.6 microg x min/mL, and stick gum - 46.4 microg x min/mL; Study 2: pellet gum - 63.0 microg x min/mL, gummy bears - 55.9 microg x min/mL, and syrup - 59.0 microg x min/mL).

Conclusion: The comparison method demonstrated high reliability and validity. In both studies other xylitol-containing products had time curves and mean xylitol concentration peaks similar to xylitol pellet gum suggesting this test may be a surrogate for longer studies comparing various products.

No MeSH data available.


Related in: MedlinePlus

Study 2: Comparison of salivary xylitol concentrations (μg/mL) after using xylitol-containing products (N = 15).
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Figure 3: Study 2: Comparison of salivary xylitol concentrations (μg/mL) after using xylitol-containing products (N = 15).

Mentions: In Study 2, the three xylitol products (pellet gum, gummy bears, and syrup) had similar time curves with a higher second peak concentration opposite to that of Study 1 (See Figure 3). Table 2 shows the mean xylitol concentrations at designated sampling points. The mean first peak was at 4 min (pellet gum – 8.2 μg/mL; gummy bears – 7.0 μg/mL) and at 6–8 min for syrup – 6.0 to 6.6 μg/mL. The mean second peak was between 13–16 min (13 min for pellet gum – 10.1 μg/mL; 14 min for gummy bears – 9.0 μg/mL; and 16 min for syrup -8.3 μg/mL). As in Study 1, the differential timing of the second peak between the xylitol products can be attributed to its different sampling protocols. Total AUC for the two xylitol-containing products did not differ significantly from the pellet gum (pellet gum – 63.0 μg.min/mL, gummy bears – 55.9 μg.min/mL, and syrup – 59.0 μg.min/mL; F(1, 26) = 0.05, p = 0.83). The correlation coefficient (r2) between the mean xylitol concentrations at each time point was 0.99 between the xylitol pellet chewing gum and gummy bears and was 0.96 between the pellet chewing gum and the syrup.


A surrogate method for comparison analysis of salivary concentrations of Xylitol-containing products.

Riedy CA, Milgrom P, Ly KA, Rothen M, Mueller G, Hagstrom MK, Tolentino E, Zhou L, Roberts MC - BMC Oral Health (2008)

Study 2: Comparison of salivary xylitol concentrations (μg/mL) after using xylitol-containing products (N = 15).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2267452&req=5

Figure 3: Study 2: Comparison of salivary xylitol concentrations (μg/mL) after using xylitol-containing products (N = 15).
Mentions: In Study 2, the three xylitol products (pellet gum, gummy bears, and syrup) had similar time curves with a higher second peak concentration opposite to that of Study 1 (See Figure 3). Table 2 shows the mean xylitol concentrations at designated sampling points. The mean first peak was at 4 min (pellet gum – 8.2 μg/mL; gummy bears – 7.0 μg/mL) and at 6–8 min for syrup – 6.0 to 6.6 μg/mL. The mean second peak was between 13–16 min (13 min for pellet gum – 10.1 μg/mL; 14 min for gummy bears – 9.0 μg/mL; and 16 min for syrup -8.3 μg/mL). As in Study 1, the differential timing of the second peak between the xylitol products can be attributed to its different sampling protocols. Total AUC for the two xylitol-containing products did not differ significantly from the pellet gum (pellet gum – 63.0 μg.min/mL, gummy bears – 55.9 μg.min/mL, and syrup – 59.0 μg.min/mL; F(1, 26) = 0.05, p = 0.83). The correlation coefficient (r2) between the mean xylitol concentrations at each time point was 0.99 between the xylitol pellet chewing gum and gummy bears and was 0.96 between the pellet chewing gum and the syrup.

Bottom Line: Study 1 had its highest mean peaks at the 4 min sampling point while Study 2 had its highest mean peaks between 13 to 16 minutes.Salivary xylitol levels returned to baseline at about 18 minutes for all forms tested.The comparison method demonstrated high reliability and validity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Northwest/Alaska Center to Reduce Oral Health Disparities and Department of Dental Public Health Sciences, School of Dentistry, University of Washington, Seattle, WA, USA. cariedy@u.washington.edu

ABSTRACT

Background: Xylitol chewing gum has been shown to reduce Streptococcus mutans levels and decay. Two studies examined the presence and time course of salivary xylitol concentrations delivered via xylitol-containing pellet gum and compared them to other xylitol-containing products.

Methods: A within-subjects design was used for both studies. Study 1, adults (N = 15) received three xylitol-containing products (pellet gum (2.6 g), gummy bears (2.6 g), and commercially available stick gum (Koolerz, 3.0 g)); Study 2, a second group of adults (N = 15) received three xylitol-containing products (pellet gum, gummy bears, and a 33% xylitol syrup (2.67 g). For both studies subjects consumed one xylitol product per visit with a 7-day washout between each product. A standardized protocol was followed for each product visit. Product order was randomly determined at the initial visit. Saliva samples (0.5 mL to 1.0 mL) were collected at baseline and up to 10 time points (approximately 16 min in length) after product consumption initiated. Concentration of xylitol in saliva samples was analyzed using high-performance liquid chromatography. Area under the curve (AUC) for determining the average xylitol concentration in saliva over the total sampling period was calculated for each product.

Results: In both studies all three xylitol products (Study 1: pellet gum, gummy bears, and stick gum; Study 2: pellet gum, gummy bears, and syrup) had similar time curves with two xylitol concentration peaks during the sampling period. Study 1 had its highest mean peaks at the 4 min sampling point while Study 2 had its highest mean peaks between 13 to 16 minutes. Salivary xylitol levels returned to baseline at about 18 minutes for all forms tested. Additionally, for both studies the total AUC for the xylitol products were similar compared to the pellet gum (Study 1: pellet gum - 51.3 microg x min/mL, gummy bears - 59.6 microg x min/mL, and stick gum - 46.4 microg x min/mL; Study 2: pellet gum - 63.0 microg x min/mL, gummy bears - 55.9 microg x min/mL, and syrup - 59.0 microg x min/mL).

Conclusion: The comparison method demonstrated high reliability and validity. In both studies other xylitol-containing products had time curves and mean xylitol concentration peaks similar to xylitol pellet gum suggesting this test may be a surrogate for longer studies comparing various products.

No MeSH data available.


Related in: MedlinePlus