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DNA-metallodrugs interactions signaled by electrochemical biosensors: an overview.

Ravera M, Bagni G, Mascini M, Osella D - Bioinorg Chem Appl (2007)

Bottom Line: The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor.Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect.The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale, Via Bellini 25g, 15100 Alessandria, Italy. mauro.ravera@mfn.unipmn.it

ABSTRACT
The interaction of drugs with DNA is an important aspect in pharmacology. In recent years, many important technological advances have been made to develop new techniques to monitor biorecognition and biointeraction on solid devices. The interaction between DNA and drugs can cause chemical and conformational modifications and, thus, variation of the electrochemical properties of nucleobases. The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor. Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect. In this context, the interaction between a panel of antitumoral Pt-, Ru-, and Ti-based metallodrugs with DNA immobilized on screen-printed electrodes has been studied. The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

No MeSH data available.


S%versus solution aging time for 0.1 mM solution ofNAMI-A in 5 mM NaCl (squares) and 100 mM NaCl (triangles) solutions,respectively, compared to the trend of the same concentration of 1 in 5 mM NaCl (circles).
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fig11: S%versus solution aging time for 0.1 mM solution ofNAMI-A in 5 mM NaCl (squares) and 100 mM NaCl (triangles) solutions,respectively, compared to the trend of the same concentration of 1 in 5 mM NaCl (circles).

Mentions: Unlike the very well-studied platinum complexes,interactions of Ti complexes with DNA are poorly understood. It seems thattitanocene dichloride TiCp2Cl2 (Figure 11) is able to interact withtransferrin, the protein associated with iron transport. In this form, titaniumactive species could cross the cell membrane, but the nature of the actualcytotoxic species remains unknown [15].


DNA-metallodrugs interactions signaled by electrochemical biosensors: an overview.

Ravera M, Bagni G, Mascini M, Osella D - Bioinorg Chem Appl (2007)

S%versus solution aging time for 0.1 mM solution ofNAMI-A in 5 mM NaCl (squares) and 100 mM NaCl (triangles) solutions,respectively, compared to the trend of the same concentration of 1 in 5 mM NaCl (circles).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2266972&req=5

fig11: S%versus solution aging time for 0.1 mM solution ofNAMI-A in 5 mM NaCl (squares) and 100 mM NaCl (triangles) solutions,respectively, compared to the trend of the same concentration of 1 in 5 mM NaCl (circles).
Mentions: Unlike the very well-studied platinum complexes,interactions of Ti complexes with DNA are poorly understood. It seems thattitanocene dichloride TiCp2Cl2 (Figure 11) is able to interact withtransferrin, the protein associated with iron transport. In this form, titaniumactive species could cross the cell membrane, but the nature of the actualcytotoxic species remains unknown [15].

Bottom Line: The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor.Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect.The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale, Via Bellini 25g, 15100 Alessandria, Italy. mauro.ravera@mfn.unipmn.it

ABSTRACT
The interaction of drugs with DNA is an important aspect in pharmacology. In recent years, many important technological advances have been made to develop new techniques to monitor biorecognition and biointeraction on solid devices. The interaction between DNA and drugs can cause chemical and conformational modifications and, thus, variation of the electrochemical properties of nucleobases. The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor. Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect. In this context, the interaction between a panel of antitumoral Pt-, Ru-, and Ti-based metallodrugs with DNA immobilized on screen-printed electrodes has been studied. The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

No MeSH data available.