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DNA-metallodrugs interactions signaled by electrochemical biosensors: an overview.

Ravera M, Bagni G, Mascini M, Osella D - Bioinorg Chem Appl (2007)

Bottom Line: The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor.Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect.The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale, Via Bellini 25g, 15100 Alessandria, Italy. mauro.ravera@mfn.unipmn.it

ABSTRACT
The interaction of drugs with DNA is an important aspect in pharmacology. In recent years, many important technological advances have been made to develop new techniques to monitor biorecognition and biointeraction on solid devices. The interaction between DNA and drugs can cause chemical and conformational modifications and, thus, variation of the electrochemical properties of nucleobases. The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor. Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect. In this context, the interaction between a panel of antitumoral Pt-, Ru-, and Ti-based metallodrugs with DNA immobilized on screen-printed electrodes has been studied. The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

No MeSH data available.


S%versus [NAMI-A] in 5 mM NaCl (squares) and 5 mMNaClO4 (triangles) solutions, respectively.
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fig10: S%versus [NAMI-A] in 5 mM NaCl (squares) and 5 mMNaClO4 (triangles) solutions, respectively.

Mentions: Also in the case of NAMI-A, S% value decreasesas concentration increases (Figure 9), but the concentration of the supportingelectrolyte plays minor roles (Figures 9 and 10). In fact, NaCl, that shouldexert mass effect, and NaClO4, that produces the noncoordinating perchlorateanion, gave similar results (Figure 9) [64]. Indeed, it is known in literature that chlorideshave a minor effect over NAMI-A aquation [79]. NAMI-A shows higher S% values incomparison with 1 , especially at low chloride concentration. Theseexperimental data further reinforce the hypothesis that DNA is not thepreferential targets of NAMI-A.


DNA-metallodrugs interactions signaled by electrochemical biosensors: an overview.

Ravera M, Bagni G, Mascini M, Osella D - Bioinorg Chem Appl (2007)

S%versus [NAMI-A] in 5 mM NaCl (squares) and 5 mMNaClO4 (triangles) solutions, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2266972&req=5

fig10: S%versus [NAMI-A] in 5 mM NaCl (squares) and 5 mMNaClO4 (triangles) solutions, respectively.
Mentions: Also in the case of NAMI-A, S% value decreasesas concentration increases (Figure 9), but the concentration of the supportingelectrolyte plays minor roles (Figures 9 and 10). In fact, NaCl, that shouldexert mass effect, and NaClO4, that produces the noncoordinating perchlorateanion, gave similar results (Figure 9) [64]. Indeed, it is known in literature that chlorideshave a minor effect over NAMI-A aquation [79]. NAMI-A shows higher S% values incomparison with 1 , especially at low chloride concentration. Theseexperimental data further reinforce the hypothesis that DNA is not thepreferential targets of NAMI-A.

Bottom Line: The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor.Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect.The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale, Via Bellini 25g, 15100 Alessandria, Italy. mauro.ravera@mfn.unipmn.it

ABSTRACT
The interaction of drugs with DNA is an important aspect in pharmacology. In recent years, many important technological advances have been made to develop new techniques to monitor biorecognition and biointeraction on solid devices. The interaction between DNA and drugs can cause chemical and conformational modifications and, thus, variation of the electrochemical properties of nucleobases. The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor. Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect. In this context, the interaction between a panel of antitumoral Pt-, Ru-, and Ti-based metallodrugs with DNA immobilized on screen-printed electrodes has been studied. The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

No MeSH data available.