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DNA-metallodrugs interactions signaled by electrochemical biosensors: an overview.

Ravera M, Bagni G, Mascini M, Osella D - Bioinorg Chem Appl (2007)

Bottom Line: The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor.Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect.The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale, Via Bellini 25g, 15100 Alessandria, Italy. mauro.ravera@mfn.unipmn.it

ABSTRACT
The interaction of drugs with DNA is an important aspect in pharmacology. In recent years, many important technological advances have been made to develop new techniques to monitor biorecognition and biointeraction on solid devices. The interaction between DNA and drugs can cause chemical and conformational modifications and, thus, variation of the electrochemical properties of nucleobases. The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor. Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect. In this context, the interaction between a panel of antitumoral Pt-, Ru-, and Ti-based metallodrugs with DNA immobilized on screen-printed electrodes has been studied. The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

No MeSH data available.


S%versus solution aging time for 0.5 mM solution of the metal complexes 1–3 in 0.25 M PB (pH = 7.4) and 5 mM NaCl.
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fig6: S%versus solution aging time for 0.5 mM solution of the metal complexes 1–3 in 0.25 M PB (pH = 7.4) and 5 mM NaCl.

Mentions: Figure 6 compares the behavior of the three metalcomplexes in identical experimental conditions (in particular at the sameconcentration) when the solution aging time is varied. As expected, a strongereffect of solution aging time on S% is observed for 1 , while, in the case of 3 , hydrolysis is not required. In fact,this complex does not need to dissociate any ancillary ligand to exert itsactivity.


DNA-metallodrugs interactions signaled by electrochemical biosensors: an overview.

Ravera M, Bagni G, Mascini M, Osella D - Bioinorg Chem Appl (2007)

S%versus solution aging time for 0.5 mM solution of the metal complexes 1–3 in 0.25 M PB (pH = 7.4) and 5 mM NaCl.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2266972&req=5

fig6: S%versus solution aging time for 0.5 mM solution of the metal complexes 1–3 in 0.25 M PB (pH = 7.4) and 5 mM NaCl.
Mentions: Figure 6 compares the behavior of the three metalcomplexes in identical experimental conditions (in particular at the sameconcentration) when the solution aging time is varied. As expected, a strongereffect of solution aging time on S% is observed for 1 , while, in the case of 3 , hydrolysis is not required. In fact,this complex does not need to dissociate any ancillary ligand to exert itsactivity.

Bottom Line: The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor.Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect.The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale, Via Bellini 25g, 15100 Alessandria, Italy. mauro.ravera@mfn.unipmn.it

ABSTRACT
The interaction of drugs with DNA is an important aspect in pharmacology. In recent years, many important technological advances have been made to develop new techniques to monitor biorecognition and biointeraction on solid devices. The interaction between DNA and drugs can cause chemical and conformational modifications and, thus, variation of the electrochemical properties of nucleobases. The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor. Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect. In this context, the interaction between a panel of antitumoral Pt-, Ru-, and Ti-based metallodrugs with DNA immobilized on screen-printed electrodes has been studied. The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

No MeSH data available.