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DNA-metallodrugs interactions signaled by electrochemical biosensors: an overview.

Ravera M, Bagni G, Mascini M, Osella D - Bioinorg Chem Appl (2007)

Bottom Line: The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor.Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect.The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale, Via Bellini 25g, 15100 Alessandria, Italy. mauro.ravera@mfn.unipmn.it

ABSTRACT
The interaction of drugs with DNA is an important aspect in pharmacology. In recent years, many important technological advances have been made to develop new techniques to monitor biorecognition and biointeraction on solid devices. The interaction between DNA and drugs can cause chemical and conformational modifications and, thus, variation of the electrochemical properties of nucleobases. The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor. Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect. In this context, the interaction between a panel of antitumoral Pt-, Ru-, and Ti-based metallodrugs with DNA immobilized on screen-printed electrodes has been studied. The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

No MeSH data available.


S%resulting from increasing concentrations of metalcomplexes in 0.25 M PB (pH = 7.4) and 5 mM NaCl (interaction time = 2 minutes).
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fig5: S%resulting from increasing concentrations of metalcomplexes in 0.25 M PB (pH = 7.4) and 5 mM NaCl (interaction time = 2 minutes).

Mentions: Figure 5 shows the S% valuesresulting from increasing concentrations of metal complexes 1 – 3 in 0.25 M phosphate buffer (PB, pH = 7.4), containing 5 mM NaCl (intracellular conditions).


DNA-metallodrugs interactions signaled by electrochemical biosensors: an overview.

Ravera M, Bagni G, Mascini M, Osella D - Bioinorg Chem Appl (2007)

S%resulting from increasing concentrations of metalcomplexes in 0.25 M PB (pH = 7.4) and 5 mM NaCl (interaction time = 2 minutes).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2266972&req=5

fig5: S%resulting from increasing concentrations of metalcomplexes in 0.25 M PB (pH = 7.4) and 5 mM NaCl (interaction time = 2 minutes).
Mentions: Figure 5 shows the S% valuesresulting from increasing concentrations of metal complexes 1 – 3 in 0.25 M phosphate buffer (PB, pH = 7.4), containing 5 mM NaCl (intracellular conditions).

Bottom Line: The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor.Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect.The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale, Via Bellini 25g, 15100 Alessandria, Italy. mauro.ravera@mfn.unipmn.it

ABSTRACT
The interaction of drugs with DNA is an important aspect in pharmacology. In recent years, many important technological advances have been made to develop new techniques to monitor biorecognition and biointeraction on solid devices. The interaction between DNA and drugs can cause chemical and conformational modifications and, thus, variation of the electrochemical properties of nucleobases. The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor. Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect. In this context, the interaction between a panel of antitumoral Pt-, Ru-, and Ti-based metallodrugs with DNA immobilized on screen-printed electrodes has been studied. The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

No MeSH data available.