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Cationic porphyrin induced a telomeric DNA to g-quadruplex form in water.

Murashima T, Sakiyama D, Miyoshi D, Kuriyama M, Yamada T, Miyazawa T, Sugimoto N - Bioinorg Chem Appl (2008)

Bottom Line: The formation of the DNA G-quadruplex is induced by the addition of a novel porphyrin carrying four cationic tethers.Circular dichroism spectroscopy reveals that the porphyrin binds to Tetrahymena telomeric repeat to form G-quadruplex under stabilizing-cation-deficient and no buffer conditions.

View Article: PubMed Central - PubMed

Affiliation: Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.

ABSTRACT
The formation of the DNA G-quadruplex is induced by the addition of a novel porphyrin carrying four cationic tethers. Circular dichroism spectroscopy reveals that the porphyrin binds to Tetrahymena telomeric repeat to form G-quadruplex under stabilizing-cation-deficient and no buffer conditions.

No MeSH data available.


Related in: MedlinePlus

CD spectra of d(T2G4)4 (25 μM) with (red) or without (black) porphyrin 5 (75 μM) in buffer containing 100 mM KCl and 50 mM Tris-HCl (pH 7.5) (a), in 100 mM KCl (b), and in water (c) at 0°C.
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fig2: CD spectra of d(T2G4)4 (25 μM) with (red) or without (black) porphyrin 5 (75 μM) in buffer containing 100 mM KCl and 50 mM Tris-HCl (pH 7.5) (a), in 100 mM KCl (b), and in water (c) at 0°C.

Mentions: Thestructure of a half-length of Tetrahymena telomere sequence, d(TG4T2G4T), was studied byCD spectroscopy. CD spectra of 50 μM of d(TG4T2G4T)(G-quadruplex concentration: 25 μM)in a 100 mM KCl solution had positive and negative peaks near 260 and 240 nm,respectively, both in the absence and presence of porphyrin 5, indicating parallel G-quadruplex (seeFigure 2(a)). These spectral features were similar to those of d(T2G4)4.On the other hand, in the absence of added cation, CD spectrum of d(TG4T2G4T)was completely different from that of d(T2G4)4 without porphyrin 5; while in thepresence of 5, the locations of positive andnegative CD signals of d(TG4T2G4T) and d(T2G4)4 were almost identical (as in Figure 2(b)). These results indicate that, whilethese two sequences have different structure in water, both are induced to formparallel G-quadruplex by the addition of cationic porphyrin 5.


Cationic porphyrin induced a telomeric DNA to g-quadruplex form in water.

Murashima T, Sakiyama D, Miyoshi D, Kuriyama M, Yamada T, Miyazawa T, Sugimoto N - Bioinorg Chem Appl (2008)

CD spectra of d(T2G4)4 (25 μM) with (red) or without (black) porphyrin 5 (75 μM) in buffer containing 100 mM KCl and 50 mM Tris-HCl (pH 7.5) (a), in 100 mM KCl (b), and in water (c) at 0°C.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC2266808&req=5

fig2: CD spectra of d(T2G4)4 (25 μM) with (red) or without (black) porphyrin 5 (75 μM) in buffer containing 100 mM KCl and 50 mM Tris-HCl (pH 7.5) (a), in 100 mM KCl (b), and in water (c) at 0°C.
Mentions: Thestructure of a half-length of Tetrahymena telomere sequence, d(TG4T2G4T), was studied byCD spectroscopy. CD spectra of 50 μM of d(TG4T2G4T)(G-quadruplex concentration: 25 μM)in a 100 mM KCl solution had positive and negative peaks near 260 and 240 nm,respectively, both in the absence and presence of porphyrin 5, indicating parallel G-quadruplex (seeFigure 2(a)). These spectral features were similar to those of d(T2G4)4.On the other hand, in the absence of added cation, CD spectrum of d(TG4T2G4T)was completely different from that of d(T2G4)4 without porphyrin 5; while in thepresence of 5, the locations of positive andnegative CD signals of d(TG4T2G4T) and d(T2G4)4 were almost identical (as in Figure 2(b)). These results indicate that, whilethese two sequences have different structure in water, both are induced to formparallel G-quadruplex by the addition of cationic porphyrin 5.

Bottom Line: The formation of the DNA G-quadruplex is induced by the addition of a novel porphyrin carrying four cationic tethers.Circular dichroism spectroscopy reveals that the porphyrin binds to Tetrahymena telomeric repeat to form G-quadruplex under stabilizing-cation-deficient and no buffer conditions.

View Article: PubMed Central - PubMed

Affiliation: Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.

ABSTRACT
The formation of the DNA G-quadruplex is induced by the addition of a novel porphyrin carrying four cationic tethers. Circular dichroism spectroscopy reveals that the porphyrin binds to Tetrahymena telomeric repeat to form G-quadruplex under stabilizing-cation-deficient and no buffer conditions.

No MeSH data available.


Related in: MedlinePlus