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Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques.

Rayavarapu RG, Petersen W, Ungureanu C, Post JN, van Leeuwen TG, Manohar S - Int J Biomed Imaging (2007)

Bottom Line: Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells.The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface.We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Biophysical Engineering Group, Institute for Biomedical Technology (BMTI), Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

ABSTRACT
We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the "optical imaging window." The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.

No MeSH data available.


Related in: MedlinePlus

Corresponding images as in Figure 7 for bioconjugates consisting ofsilver-stained HER81/gold nanorod conjugates incubated with (a) SKBR3 cells and(b) CHO cells. Care was taken to maintain the same acquisition parameters inboth cases. The bioconjugates are accumulated at the cell membranes of SKBR3cells where HER2 is localized. As expected, no such accumulation takes place inthe case of HER2 negative CHO cells.
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fig8: Corresponding images as in Figure 7 for bioconjugates consisting ofsilver-stained HER81/gold nanorod conjugates incubated with (a) SKBR3 cells and(b) CHO cells. Care was taken to maintain the same acquisition parameters inboth cases. The bioconjugates are accumulated at the cell membranes of SKBR3cells where HER2 is localized. As expected, no such accumulation takes place inthe case of HER2 negative CHO cells.

Mentions: Figures 8(a) and 8(b) are the results of thecorresponding controls using the HER81 mAb/gold nanorods.


Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques.

Rayavarapu RG, Petersen W, Ungureanu C, Post JN, van Leeuwen TG, Manohar S - Int J Biomed Imaging (2007)

Corresponding images as in Figure 7 for bioconjugates consisting ofsilver-stained HER81/gold nanorod conjugates incubated with (a) SKBR3 cells and(b) CHO cells. Care was taken to maintain the same acquisition parameters inboth cases. The bioconjugates are accumulated at the cell membranes of SKBR3cells where HER2 is localized. As expected, no such accumulation takes place inthe case of HER2 negative CHO cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2266791&req=5

fig8: Corresponding images as in Figure 7 for bioconjugates consisting ofsilver-stained HER81/gold nanorod conjugates incubated with (a) SKBR3 cells and(b) CHO cells. Care was taken to maintain the same acquisition parameters inboth cases. The bioconjugates are accumulated at the cell membranes of SKBR3cells where HER2 is localized. As expected, no such accumulation takes place inthe case of HER2 negative CHO cells.
Mentions: Figures 8(a) and 8(b) are the results of thecorresponding controls using the HER81 mAb/gold nanorods.

Bottom Line: Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells.The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface.We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Biophysical Engineering Group, Institute for Biomedical Technology (BMTI), Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

ABSTRACT
We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the "optical imaging window." The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.

No MeSH data available.


Related in: MedlinePlus