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Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques.

Rayavarapu RG, Petersen W, Ungureanu C, Post JN, van Leeuwen TG, Manohar S - Int J Biomed Imaging (2007)

Bottom Line: Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells.The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface.We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Biophysical Engineering Group, Institute for Biomedical Technology (BMTI), Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

ABSTRACT
We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the "optical imaging window." The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.

No MeSH data available.


Related in: MedlinePlus

Extinction spectra before and after incubation ofHER81 with (a) gold nanospheres, (b) gold nanorods. In both cases, a red shiftin plasmon band(s) occurs after incubation with the antibody signifyingsuccessful bioconjugation.
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fig6: Extinction spectra before and after incubation ofHER81 with (a) gold nanospheres, (b) gold nanorods. In both cases, a red shiftin plasmon band(s) occurs after incubation with the antibody signifyingsuccessful bioconjugation.

Mentions: Figure 6(a) shows the extinction spectrum of goldnanospheres before and after incubation with HER81. Figure 6(b) is thecorresponding situation with gold nanorods before and after incubation with theHER81 mAb. In both cases, the characteristic red-shift in the extinction peakof the plasmon bands is seen.


Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques.

Rayavarapu RG, Petersen W, Ungureanu C, Post JN, van Leeuwen TG, Manohar S - Int J Biomed Imaging (2007)

Extinction spectra before and after incubation ofHER81 with (a) gold nanospheres, (b) gold nanorods. In both cases, a red shiftin plasmon band(s) occurs after incubation with the antibody signifyingsuccessful bioconjugation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2266791&req=5

fig6: Extinction spectra before and after incubation ofHER81 with (a) gold nanospheres, (b) gold nanorods. In both cases, a red shiftin plasmon band(s) occurs after incubation with the antibody signifyingsuccessful bioconjugation.
Mentions: Figure 6(a) shows the extinction spectrum of goldnanospheres before and after incubation with HER81. Figure 6(b) is thecorresponding situation with gold nanorods before and after incubation with theHER81 mAb. In both cases, the characteristic red-shift in the extinction peakof the plasmon bands is seen.

Bottom Line: Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells.The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface.We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Biophysical Engineering Group, Institute for Biomedical Technology (BMTI), Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

ABSTRACT
We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the "optical imaging window." The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.

No MeSH data available.


Related in: MedlinePlus