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Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques.

Rayavarapu RG, Petersen W, Ungureanu C, Post JN, van Leeuwen TG, Manohar S - Int J Biomed Imaging (2007)

Bottom Line: Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells.The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface.We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Biophysical Engineering Group, Institute for Biomedical Technology (BMTI), Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

ABSTRACT
We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the "optical imaging window." The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.

No MeSH data available.


Related in: MedlinePlus

Histograms of gold nanorod aspect ratios synthesized with (a) 50 μL silver nitrate, mean aspect ratio of 2.3 ± 0.3 (mean length 44.8 ± 4.1nm, mean width 19.8 ± 2.9 nm); and with (b) 250 μL silver nitrate, mean aspect ratio of 3.6 ± 0.6 (mean length 51.0± 4.4 nm, mean width 14.1±2.1).
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fig4: Histograms of gold nanorod aspect ratios synthesized with (a) 50 μL silver nitrate, mean aspect ratio of 2.3 ± 0.3 (mean length 44.8 ± 4.1nm, mean width 19.8 ± 2.9 nm); and with (b) 250 μL silver nitrate, mean aspect ratio of 3.6 ± 0.6 (mean length 51.0± 4.4 nm, mean width 14.1±2.1).

Mentions: Examination of the SEM image and determination of themean sizes confirm that the NPs produced consist of monodisperse nanorods ofaspect ratio of 2.3 ± 0.3, with a small number of large spheres; the latter'sextinction peak coinciding with the transverse plasmon band of the nanorods.Figure 3 shows the extinction spectrum and the SEM image for the sampleproduced using 250 μL of silvernitrate. It is seen that the longitudinal plasmon band is shifted to 850 nm.Sizing from the SEM image yields an average aspect ratio of 3.6 ± 0.6. Figure 4 shows the size distribution of the 2specimens.


Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques.

Rayavarapu RG, Petersen W, Ungureanu C, Post JN, van Leeuwen TG, Manohar S - Int J Biomed Imaging (2007)

Histograms of gold nanorod aspect ratios synthesized with (a) 50 μL silver nitrate, mean aspect ratio of 2.3 ± 0.3 (mean length 44.8 ± 4.1nm, mean width 19.8 ± 2.9 nm); and with (b) 250 μL silver nitrate, mean aspect ratio of 3.6 ± 0.6 (mean length 51.0± 4.4 nm, mean width 14.1±2.1).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2266791&req=5

fig4: Histograms of gold nanorod aspect ratios synthesized with (a) 50 μL silver nitrate, mean aspect ratio of 2.3 ± 0.3 (mean length 44.8 ± 4.1nm, mean width 19.8 ± 2.9 nm); and with (b) 250 μL silver nitrate, mean aspect ratio of 3.6 ± 0.6 (mean length 51.0± 4.4 nm, mean width 14.1±2.1).
Mentions: Examination of the SEM image and determination of themean sizes confirm that the NPs produced consist of monodisperse nanorods ofaspect ratio of 2.3 ± 0.3, with a small number of large spheres; the latter'sextinction peak coinciding with the transverse plasmon band of the nanorods.Figure 3 shows the extinction spectrum and the SEM image for the sampleproduced using 250 μL of silvernitrate. It is seen that the longitudinal plasmon band is shifted to 850 nm.Sizing from the SEM image yields an average aspect ratio of 3.6 ± 0.6. Figure 4 shows the size distribution of the 2specimens.

Bottom Line: Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells.The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface.We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Biophysical Engineering Group, Institute for Biomedical Technology (BMTI), Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

ABSTRACT
We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the "optical imaging window." The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.

No MeSH data available.


Related in: MedlinePlus