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Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques.

Rayavarapu RG, Petersen W, Ungureanu C, Post JN, van Leeuwen TG, Manohar S - Int J Biomed Imaging (2007)

Bottom Line: Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells.The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface.We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Biophysical Engineering Group, Institute for Biomedical Technology (BMTI), Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

ABSTRACT
We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the "optical imaging window." The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.

No MeSH data available.


Related in: MedlinePlus

Gold nanorods synthesized using 50 μL of silver nitrate in growth solution. (a) Optical extinction spectrum showing the transverse plasmon peak at 516.5 nm and the longitudinal plasmon peak at 675nm. The amplitude of the longitudinal plasmon peak is higher than transverseplasmon peak which indicates the formation of high yield of nanorods comparedto spheres. (b) High-resolution scanning electron microscope (SEM) image ofgold nanorods showing high monodispersity. Few nanospheres are observed.
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fig2: Gold nanorods synthesized using 50 μL of silver nitrate in growth solution. (a) Optical extinction spectrum showing the transverse plasmon peak at 516.5 nm and the longitudinal plasmon peak at 675nm. The amplitude of the longitudinal plasmon peak is higher than transverseplasmon peak which indicates the formation of high yield of nanorods comparedto spheres. (b) High-resolution scanning electron microscope (SEM) image ofgold nanorods showing high monodispersity. Few nanospheres are observed.

Mentions: Figure 2 shows the extinction spectrum and HR-SEMimage of the nanorods synthesized using 50 μL AgNO3 in thegrowth solution. The peak at 675 nm can be attributed to longitudinal plasmonresonance and the peak in the vicinity of 516.5 nm to transverse plasmonresonance.


Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques.

Rayavarapu RG, Petersen W, Ungureanu C, Post JN, van Leeuwen TG, Manohar S - Int J Biomed Imaging (2007)

Gold nanorods synthesized using 50 μL of silver nitrate in growth solution. (a) Optical extinction spectrum showing the transverse plasmon peak at 516.5 nm and the longitudinal plasmon peak at 675nm. The amplitude of the longitudinal plasmon peak is higher than transverseplasmon peak which indicates the formation of high yield of nanorods comparedto spheres. (b) High-resolution scanning electron microscope (SEM) image ofgold nanorods showing high monodispersity. Few nanospheres are observed.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2266791&req=5

fig2: Gold nanorods synthesized using 50 μL of silver nitrate in growth solution. (a) Optical extinction spectrum showing the transverse plasmon peak at 516.5 nm and the longitudinal plasmon peak at 675nm. The amplitude of the longitudinal plasmon peak is higher than transverseplasmon peak which indicates the formation of high yield of nanorods comparedto spheres. (b) High-resolution scanning electron microscope (SEM) image ofgold nanorods showing high monodispersity. Few nanospheres are observed.
Mentions: Figure 2 shows the extinction spectrum and HR-SEMimage of the nanorods synthesized using 50 μL AgNO3 in thegrowth solution. The peak at 675 nm can be attributed to longitudinal plasmonresonance and the peak in the vicinity of 516.5 nm to transverse plasmonresonance.

Bottom Line: Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells.The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface.We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: Biophysical Engineering Group, Institute for Biomedical Technology (BMTI), Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

ABSTRACT
We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the "optical imaging window." The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.

No MeSH data available.


Related in: MedlinePlus