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An in vitro method to study the effects of hematopoietic regulators during immune and blood cell development.

Patel N, Castillo M, Rameshwar P - Biol Proced Online (2007)

Bottom Line: Methods on hematopoietic regulation are presented to show the effects of the chemokine, stromal-derived growth factor (SDF)-1I and the neuropeptide, substance P (SP).The indirect method occurs by SP production, which stimulates CD34+ cells, supported by ligand-binding studies, long-term culture-initiating cell assays for HSC functions, and clonogenic assays for myeloid progenitors.These methods can be applied to study other hematopoietic regulators.

View Article: PubMed Central - PubMed

Affiliation: UMDNJ-New Jersey Medical School, MSB, Rm. E-579 185 South Orange Ave, Newark, NJ 07103, USA.

ABSTRACT
In adults, hematopoiesis occurs in bone marrow (BM) through a complex process with differentiation of hematopoietic stem cells (HSCs) to immune and blood cells. Human HSCs and their progenitors express CD34. Methods on hematopoietic regulation are presented to show the effects of the chemokine, stromal-derived growth factor (SDF)-1I and the neuropeptide, substance P (SP). SDF-1I production in BM stroma causes interactions with HSCs, thereby retaining the HSCs in regions close to the endosteum, at low oxygen. Small changes in SDF-1I levels stimulate HSC functions through direct and indirect mechanisms. The indirect method occurs by SP production, which stimulates CD34+ cells, supported by ligand-binding studies, long-term culture-initiating cell assays for HSC functions, and clonogenic assays for myeloid progenitors. These methods can be applied to study other hematopoietic regulators.

No MeSH data available.


Fig. 2The effects of SP, NK-A and SDF-1Î on hematopoiesis. BM stroma is innervated by peptidergic fibers, which can release two neuropeptides, SP and NK-A. SP interacts with BM Stroma via a specific receptor, NK1, which induces growth factors that stimulate hematopoiesis. NKA can also be produced by peptidergic fibers, which interact with NK2 on BM Stroma, which causes inhibition of hematopoiesis. High levels of SDF-1Î stimulate BM Stroma to induced SP production, which stimulates hematopoiesis.
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f2: Fig. 2The effects of SP, NK-A and SDF-1Î on hematopoiesis. BM stroma is innervated by peptidergic fibers, which can release two neuropeptides, SP and NK-A. SP interacts with BM Stroma via a specific receptor, NK1, which induces growth factors that stimulate hematopoiesis. NKA can also be produced by peptidergic fibers, which interact with NK2 on BM Stroma, which causes inhibition of hematopoiesis. High levels of SDF-1Î stimulate BM Stroma to induced SP production, which stimulates hematopoiesis.


An in vitro method to study the effects of hematopoietic regulators during immune and blood cell development.

Patel N, Castillo M, Rameshwar P - Biol Proced Online (2007)

Fig. 2The effects of SP, NK-A and SDF-1Î on hematopoiesis. BM stroma is innervated by peptidergic fibers, which can release two neuropeptides, SP and NK-A. SP interacts with BM Stroma via a specific receptor, NK1, which induces growth factors that stimulate hematopoiesis. NKA can also be produced by peptidergic fibers, which interact with NK2 on BM Stroma, which causes inhibition of hematopoiesis. High levels of SDF-1Î stimulate BM Stroma to induced SP production, which stimulates hematopoiesis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2266633&req=5

f2: Fig. 2The effects of SP, NK-A and SDF-1Î on hematopoiesis. BM stroma is innervated by peptidergic fibers, which can release two neuropeptides, SP and NK-A. SP interacts with BM Stroma via a specific receptor, NK1, which induces growth factors that stimulate hematopoiesis. NKA can also be produced by peptidergic fibers, which interact with NK2 on BM Stroma, which causes inhibition of hematopoiesis. High levels of SDF-1Î stimulate BM Stroma to induced SP production, which stimulates hematopoiesis.
Bottom Line: Methods on hematopoietic regulation are presented to show the effects of the chemokine, stromal-derived growth factor (SDF)-1I and the neuropeptide, substance P (SP).The indirect method occurs by SP production, which stimulates CD34+ cells, supported by ligand-binding studies, long-term culture-initiating cell assays for HSC functions, and clonogenic assays for myeloid progenitors.These methods can be applied to study other hematopoietic regulators.

View Article: PubMed Central - PubMed

Affiliation: UMDNJ-New Jersey Medical School, MSB, Rm. E-579 185 South Orange Ave, Newark, NJ 07103, USA.

ABSTRACT
In adults, hematopoiesis occurs in bone marrow (BM) through a complex process with differentiation of hematopoietic stem cells (HSCs) to immune and blood cells. Human HSCs and their progenitors express CD34. Methods on hematopoietic regulation are presented to show the effects of the chemokine, stromal-derived growth factor (SDF)-1I and the neuropeptide, substance P (SP). SDF-1I production in BM stroma causes interactions with HSCs, thereby retaining the HSCs in regions close to the endosteum, at low oxygen. Small changes in SDF-1I levels stimulate HSC functions through direct and indirect mechanisms. The indirect method occurs by SP production, which stimulates CD34+ cells, supported by ligand-binding studies, long-term culture-initiating cell assays for HSC functions, and clonogenic assays for myeloid progenitors. These methods can be applied to study other hematopoietic regulators.

No MeSH data available.