Limits...
An in vitro method to study the effects of hematopoietic regulators during immune and blood cell development.

Patel N, Castillo M, Rameshwar P - Biol Proced Online (2007)

Bottom Line: Methods on hematopoietic regulation are presented to show the effects of the chemokine, stromal-derived growth factor (SDF)-1I and the neuropeptide, substance P (SP).Small changes in SDF-1I levels stimulate HSC functions through direct and indirect mechanisms.These methods can be applied to study other hematopoietic regulators.

View Article: PubMed Central - PubMed

Affiliation: UMDNJ-New Jersey Medical School, MSB, Rm. E-579 185 South Orange Ave, Newark, NJ 07103, USA.

ABSTRACT
In adults, hematopoiesis occurs in bone marrow (BM) through a complex process with differentiation of hematopoietic stem cells (HSCs) to immune and blood cells. Human HSCs and their progenitors express CD34. Methods on hematopoietic regulation are presented to show the effects of the chemokine, stromal-derived growth factor (SDF)-1I and the neuropeptide, substance P (SP). SDF-1I production in BM stroma causes interactions with HSCs, thereby retaining the HSCs in regions close to the endosteum, at low oxygen. Small changes in SDF-1I levels stimulate HSC functions through direct and indirect mechanisms. The indirect method occurs by SP production, which stimulates CD34+ cells, supported by ligand-binding studies, long-term culture-initiating cell assays for HSC functions, and clonogenic assays for myeloid progenitors. These methods can be applied to study other hematopoietic regulators.

No MeSH data available.


Fig. 1Scatchard analysis of SP-binding sites on CD34+ cells. We performed ligand-binding studies with 125I-Tyr-SP to confirm SP is capable of binding to CD34+ cells and also to determine the binding kinetics of SP on these cells.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2266633&req=5

f1: Fig. 1Scatchard analysis of SP-binding sites on CD34+ cells. We performed ligand-binding studies with 125I-Tyr-SP to confirm SP is capable of binding to CD34+ cells and also to determine the binding kinetics of SP on these cells.


An in vitro method to study the effects of hematopoietic regulators during immune and blood cell development.

Patel N, Castillo M, Rameshwar P - Biol Proced Online (2007)

Fig. 1Scatchard analysis of SP-binding sites on CD34+ cells. We performed ligand-binding studies with 125I-Tyr-SP to confirm SP is capable of binding to CD34+ cells and also to determine the binding kinetics of SP on these cells.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2266633&req=5

f1: Fig. 1Scatchard analysis of SP-binding sites on CD34+ cells. We performed ligand-binding studies with 125I-Tyr-SP to confirm SP is capable of binding to CD34+ cells and also to determine the binding kinetics of SP on these cells.
Bottom Line: Methods on hematopoietic regulation are presented to show the effects of the chemokine, stromal-derived growth factor (SDF)-1I and the neuropeptide, substance P (SP).Small changes in SDF-1I levels stimulate HSC functions through direct and indirect mechanisms.These methods can be applied to study other hematopoietic regulators.

View Article: PubMed Central - PubMed

Affiliation: UMDNJ-New Jersey Medical School, MSB, Rm. E-579 185 South Orange Ave, Newark, NJ 07103, USA.

ABSTRACT
In adults, hematopoiesis occurs in bone marrow (BM) through a complex process with differentiation of hematopoietic stem cells (HSCs) to immune and blood cells. Human HSCs and their progenitors express CD34. Methods on hematopoietic regulation are presented to show the effects of the chemokine, stromal-derived growth factor (SDF)-1I and the neuropeptide, substance P (SP). SDF-1I production in BM stroma causes interactions with HSCs, thereby retaining the HSCs in regions close to the endosteum, at low oxygen. Small changes in SDF-1I levels stimulate HSC functions through direct and indirect mechanisms. The indirect method occurs by SP production, which stimulates CD34+ cells, supported by ligand-binding studies, long-term culture-initiating cell assays for HSC functions, and clonogenic assays for myeloid progenitors. These methods can be applied to study other hematopoietic regulators.

No MeSH data available.