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Role of plasma protein and low-molecular weight substances in the change of hydroxyl radical scavenging activity in hemodialysis patients.

Hirakawa A, Aoyagi K, Nakajima M, Kikuchi S, Ohba S, Yamagata K - J Clin Biochem Nutr (2008)

Bottom Line: In 5 patients however, their p-HRSA decreased after HD, in spite of increasing TP.In pre-HD, the p-HRSA values and hydroxyl radical scavenging activity of low-molecular weight fraction of plasma were significantly higher in these 5 patients than in patients whose p-HRSA increased after HD.These findings suggest that p-HRSA is mainly affected by TP, but caution should be exercised in patients who have high p-HRSA before HD and whose p-HRSA does not increase after HD.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-0006, Japan.

ABSTRACT
While it is well known that hemodialysis (HD) patients with end stage renal failure are exposed to high oxidative stress, there is not a general opinion regarding whether antioxidant activity is high or low in these patients. We evaluated the variation of plasma hydroxyl radical scavenging activity (p-HRSA) by a single-session of HD in 69 patients by using a new system, reactive flow-injection electron spin resonance. And then comparing p-HRSA with their biochemical parameters, we tried to elucidate what components affected p-HRSA in the HD patients. The average of p-HRSA significantly increased after HD and the variation of p-HRSA by HD was correlated with that of plasma total protein (TP). In 5 patients however, their p-HRSA decreased after HD, in spite of increasing TP. In pre-HD, the p-HRSA values and hydroxyl radical scavenging activity of low-molecular weight fraction of plasma were significantly higher in these 5 patients than in patients whose p-HRSA increased after HD. These 5 patients were in an inflammatory state. These findings suggest that p-HRSA is mainly affected by TP, but caution should be exercised in patients who have high p-HRSA before HD and whose p-HRSA does not increase after HD.

No MeSH data available.


Related in: MedlinePlus

Correlation between plasma concentration of albumin and p-HRSA value in pre-HD and post-HD. Pre-HD are shown by closed circles (r = 0.02, p = 0.88), post-HD are open circles (r = 0.45, p<0.0001)
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Figure 6: Correlation between plasma concentration of albumin and p-HRSA value in pre-HD and post-HD. Pre-HD are shown by closed circles (r = 0.02, p = 0.88), post-HD are open circles (r = 0.45, p<0.0001)

Mentions: Plasma protein includes many kinds of proteins but albumin is known to be the most abundant protein. We also investigated the possibility that there is correlation between p-HRSA value and plasma albumin. Before HD they were not correlated (r = 0.02, p = 0.88), whereas after HD, p-HRSA values were correlated with plasma albumin levels (r = 0.45, p<0.0001) (Fig. 6).


Role of plasma protein and low-molecular weight substances in the change of hydroxyl radical scavenging activity in hemodialysis patients.

Hirakawa A, Aoyagi K, Nakajima M, Kikuchi S, Ohba S, Yamagata K - J Clin Biochem Nutr (2008)

Correlation between plasma concentration of albumin and p-HRSA value in pre-HD and post-HD. Pre-HD are shown by closed circles (r = 0.02, p = 0.88), post-HD are open circles (r = 0.45, p<0.0001)
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2266055&req=5

Figure 6: Correlation between plasma concentration of albumin and p-HRSA value in pre-HD and post-HD. Pre-HD are shown by closed circles (r = 0.02, p = 0.88), post-HD are open circles (r = 0.45, p<0.0001)
Mentions: Plasma protein includes many kinds of proteins but albumin is known to be the most abundant protein. We also investigated the possibility that there is correlation between p-HRSA value and plasma albumin. Before HD they were not correlated (r = 0.02, p = 0.88), whereas after HD, p-HRSA values were correlated with plasma albumin levels (r = 0.45, p<0.0001) (Fig. 6).

Bottom Line: In 5 patients however, their p-HRSA decreased after HD, in spite of increasing TP.In pre-HD, the p-HRSA values and hydroxyl radical scavenging activity of low-molecular weight fraction of plasma were significantly higher in these 5 patients than in patients whose p-HRSA increased after HD.These findings suggest that p-HRSA is mainly affected by TP, but caution should be exercised in patients who have high p-HRSA before HD and whose p-HRSA does not increase after HD.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-0006, Japan.

ABSTRACT
While it is well known that hemodialysis (HD) patients with end stage renal failure are exposed to high oxidative stress, there is not a general opinion regarding whether antioxidant activity is high or low in these patients. We evaluated the variation of plasma hydroxyl radical scavenging activity (p-HRSA) by a single-session of HD in 69 patients by using a new system, reactive flow-injection electron spin resonance. And then comparing p-HRSA with their biochemical parameters, we tried to elucidate what components affected p-HRSA in the HD patients. The average of p-HRSA significantly increased after HD and the variation of p-HRSA by HD was correlated with that of plasma total protein (TP). In 5 patients however, their p-HRSA decreased after HD, in spite of increasing TP. In pre-HD, the p-HRSA values and hydroxyl radical scavenging activity of low-molecular weight fraction of plasma were significantly higher in these 5 patients than in patients whose p-HRSA increased after HD. These 5 patients were in an inflammatory state. These findings suggest that p-HRSA is mainly affected by TP, but caution should be exercised in patients who have high p-HRSA before HD and whose p-HRSA does not increase after HD.

No MeSH data available.


Related in: MedlinePlus