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Early antibody response against Mycobacterium avium subspecies paratuberculosis antigens in subclinical cattle.

Bannantine JP, Bayles DO, Waters WR, Palmer MV, Stabel JR, Paustian ML - Proteome Sci (2008)

Bottom Line: The most reactive protein was a putative surface antigen encoded by MAP1087.A second protein, MAP1204, implicated in virulence, was also strongly detected by day 70 in both cattle.Subsequent experiments showed that these two proteins were detected with sera from 5 of 9 naturally infected cattle in the subclinical stage of Johne's disease.

View Article: PubMed Central - HTML - PubMed

Affiliation: USDA/ARS/National Animal Disease Center, Ames, IA 50010, USA. john.bannantine@ars.usda.gov

ABSTRACT

Background: Our laboratories have previously reported on the experimental infection of cattle with Mycobacterium avium subsp paratuberculosis (M. paratuberculosis) using an intratonsillar infection model. In addition, we have recently developed a partial protein array representing 92 M. paratuberculosis coding sequences. These combined tools have enabled a unique look at the temporal analysis of M. paratuberculosis antigens within the native host. The primary objective of this study was to identify M. paratuberculosis antigens detected by cattle early during infection. A secondary objective was to evaluate the humoral immune response in cattle during the initial year of infection.

Results: Sera from two experimentally infected cattle, taken pre-inoculation and at day 70, 194 and 321 post infection, identified dynamic antibody reactivity among antigens with some showing an increased response over time and others showing declining levels of reactivity over the same time period. A M. paratuberculosis specific protein, encoded by MAP0862, was strongly detected initially, but the antibody response became weaker with time. The most reactive protein was a putative surface antigen encoded by MAP1087. A second protein, MAP1204, implicated in virulence, was also strongly detected by day 70 in both cattle. Subsequent experiments showed that these two proteins were detected with sera from 5 of 9 naturally infected cattle in the subclinical stage of Johne's disease.

Conclusion: Collectively these results demonstrate that M. paratuberculosis proteins are detected by sera from experimentally infected cattle as early as 70 days after exposure. These data further suggest at least two antigens may be useful in the early diagnosis of M. paratuberculosis infections. Finally, the construction and use of a protein array in this pilot study has led to a novel approach for discovery of M. paratuberculosis antigens.

No MeSH data available.


Related in: MedlinePlus

Total number of M. paratuberculosis recombinant proteins detected by sera from experimentally infected cattle. The graph shows the number of proteins on the 96-dot array (y-axis) that were detected at the day post infection (x-axis) for each calf. The total number of proteins detected is indicated on each bar. Note that similar numbers of proteins were detected by each animal at the end of the study period.
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Figure 1: Total number of M. paratuberculosis recombinant proteins detected by sera from experimentally infected cattle. The graph shows the number of proteins on the 96-dot array (y-axis) that were detected at the day post infection (x-axis) for each calf. The total number of proteins detected is indicated on each bar. Note that similar numbers of proteins were detected by each animal at the end of the study period.

Mentions: An analysis of serial bleeds collected from two experimentally infected calves was performed to temporally examine the humoral immune response to the arrayed M. paratuberculosis proteins. In general, the antibody response against the set of 92 proteins was remarkably similar between the two animals. One notable difference was that fewer proteins were recognized at day 70 in animal 5904 compared to 5902, as only 11 proteins compared to 27 proteins were detected, respectively (Figure 1 and Figure 2). However by day 194, animal 5904 had antibodies against more M. paratuberculosis proteins than did animal 5902. Serum from both animals detected similar numbers of proteins at the end of the study period (Figure 1). The pre-infection sera showed no detection of any of the recombinant proteins (Figure 2) and there was no detection of the MBP-LacZ protein for all time points suggesting that these calves do not have antibodies to the maltose binding protein (MBP) affinity purification tag.


Early antibody response against Mycobacterium avium subspecies paratuberculosis antigens in subclinical cattle.

Bannantine JP, Bayles DO, Waters WR, Palmer MV, Stabel JR, Paustian ML - Proteome Sci (2008)

Total number of M. paratuberculosis recombinant proteins detected by sera from experimentally infected cattle. The graph shows the number of proteins on the 96-dot array (y-axis) that were detected at the day post infection (x-axis) for each calf. The total number of proteins detected is indicated on each bar. Note that similar numbers of proteins were detected by each animal at the end of the study period.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2265687&req=5

Figure 1: Total number of M. paratuberculosis recombinant proteins detected by sera from experimentally infected cattle. The graph shows the number of proteins on the 96-dot array (y-axis) that were detected at the day post infection (x-axis) for each calf. The total number of proteins detected is indicated on each bar. Note that similar numbers of proteins were detected by each animal at the end of the study period.
Mentions: An analysis of serial bleeds collected from two experimentally infected calves was performed to temporally examine the humoral immune response to the arrayed M. paratuberculosis proteins. In general, the antibody response against the set of 92 proteins was remarkably similar between the two animals. One notable difference was that fewer proteins were recognized at day 70 in animal 5904 compared to 5902, as only 11 proteins compared to 27 proteins were detected, respectively (Figure 1 and Figure 2). However by day 194, animal 5904 had antibodies against more M. paratuberculosis proteins than did animal 5902. Serum from both animals detected similar numbers of proteins at the end of the study period (Figure 1). The pre-infection sera showed no detection of any of the recombinant proteins (Figure 2) and there was no detection of the MBP-LacZ protein for all time points suggesting that these calves do not have antibodies to the maltose binding protein (MBP) affinity purification tag.

Bottom Line: The most reactive protein was a putative surface antigen encoded by MAP1087.A second protein, MAP1204, implicated in virulence, was also strongly detected by day 70 in both cattle.Subsequent experiments showed that these two proteins were detected with sera from 5 of 9 naturally infected cattle in the subclinical stage of Johne's disease.

View Article: PubMed Central - HTML - PubMed

Affiliation: USDA/ARS/National Animal Disease Center, Ames, IA 50010, USA. john.bannantine@ars.usda.gov

ABSTRACT

Background: Our laboratories have previously reported on the experimental infection of cattle with Mycobacterium avium subsp paratuberculosis (M. paratuberculosis) using an intratonsillar infection model. In addition, we have recently developed a partial protein array representing 92 M. paratuberculosis coding sequences. These combined tools have enabled a unique look at the temporal analysis of M. paratuberculosis antigens within the native host. The primary objective of this study was to identify M. paratuberculosis antigens detected by cattle early during infection. A secondary objective was to evaluate the humoral immune response in cattle during the initial year of infection.

Results: Sera from two experimentally infected cattle, taken pre-inoculation and at day 70, 194 and 321 post infection, identified dynamic antibody reactivity among antigens with some showing an increased response over time and others showing declining levels of reactivity over the same time period. A M. paratuberculosis specific protein, encoded by MAP0862, was strongly detected initially, but the antibody response became weaker with time. The most reactive protein was a putative surface antigen encoded by MAP1087. A second protein, MAP1204, implicated in virulence, was also strongly detected by day 70 in both cattle. Subsequent experiments showed that these two proteins were detected with sera from 5 of 9 naturally infected cattle in the subclinical stage of Johne's disease.

Conclusion: Collectively these results demonstrate that M. paratuberculosis proteins are detected by sera from experimentally infected cattle as early as 70 days after exposure. These data further suggest at least two antigens may be useful in the early diagnosis of M. paratuberculosis infections. Finally, the construction and use of a protein array in this pilot study has led to a novel approach for discovery of M. paratuberculosis antigens.

No MeSH data available.


Related in: MedlinePlus